NaCl‚0.6M NaCl‚1.2M NaC ‚4.8M NaCl). Their rationale hypothesis “An increase of sodium chloride (NaCl) will decrease the proteolytic activity of trypsin based on the effect salt concentration has on protein denaturation” was proven throughout their experiment to be accepted (Theo Balog‚ Samantha Lin‚ Derek Margulies‚ Kayla McCulloch‚ Group Five:
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RECRYSTALLIZATION AND MELTING POINT DETERMINATION OF BENZOIC ACID ANSWERS TO QUESTIONS 1. How does the use of fluted filter paper hasten filtration? Why is it advisable to place a small piece of wire between the funnel and the mouth of the flask during hot filtration? Fluted filter paper is effective in preventing crystal formation in the filter paper. It is also often used in filtering hot saturated solution used during crystallization. One major advantage of a fluted filter is that it increases
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TITLE OF EXPERIMENT : DETERMINATION OF THE RELATIVE MOLECULAR MASS OF AMIDOSULPHURIC ACID DATA COLLECTION AND PROCESSING. QUANTITATIVE DATA Type equation here. Experiment | Burette reading / cm3 | Volume of solution used(±0.1 cm3) | | Initial reading(± 0.05 cm3) | Final reading(± 0.05 cm3) | | 1 | 0.00 | 24.70 | 24.7 | 2 | 18.20 | 48.00 | 29.8 | 3 | 6.00 | 33.00 | 27.0 | QUALITATIVE DATA 1. When amidosulphuric acid was diluted
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Determination of An Unknown Amino Acid From Titration Abstract Experiment 11 used a titration curve to determine the identity of an unknown amino acid. The initial pH of the solution was 1.96‚ and the pKa’s found experimentally were 2.0‚ 4.0‚ and 9.85. The accepted pKa values were found to be 2.10‚ 4.07‚ and 9.47. The molecular weight was calculated to be 176.3 while the accepted value was found to be 183.5. The identity of the unknown amino acid was established to be glutamic acid‚
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by reacting nitric and sulfuric acids. After the nucleophile adds‚ the ring has lost aromaticity. Therefore‚ the deprotonated acid in solution can pull off a hydrogen from the same carbon that the nitro group has added to‚ allowing the electrons from that bond to go back into the ring to reproduce aromaticty. There are three possible positions on a benzene ring that a nucleophile could add to‚ referred to as the ortho‚ para‚ or meta positions. In this experiment‚ the nucleophile will primarily
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Nitration of Methyl Benzoate Abstract: This procedure demonstrates the nitration of methyl benzoate to prepare methyl m-nitrobenzoate. Methyl benzoate was treated with concentrated Nitric and Sulfuric acid to yield methyl m-nitrobenzoate. The product was then isolated and recrystallized using methanol. This reaction is an example of an electrophilic aromatic substitution reaction‚ in which the nitro group replaces a proton of the aromatic ring. Following recrystallization‚ melting point and infrared
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Nitration of Methyl Benzoate Introduction: Nitration is an example of an electrophile aromatic substitution reaction‚ where nitro (NO2) group is being substituted for a hydrogen on an aromatic compound. This is achieved by the formation of the nitronium ion by protonation of nitric acid from sulfuric acid. The zirconium ion is a strong electrophile and can react with aromatic compound such as Methyl benzoate to form an arenium ion intermediate. The arenium ion is then depronated to reform
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family‚ a group of compounds that possess antibacterial and antifungal properties. These agents are esters of para-hydroxybenzoic acid‚ which is why they are collectively called parabens. However‚ in contrast to its cousins‚ ethylparaben‚ butylparaben‚ and propylparaben‚ methylparaben receives its specific name owing to the fact that its chemical structure contains the methyl alkyl group. Methylparaben is found in several fruits‚ in particular blueberries‚ where it acts as an antimicrobial agent. Methylparaben
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To synthesis methyl orange by coupling diazotised sulphanilic acid with N‚N-dimethylaniline. Materials (Chemicals) Sulfanilic acid‚ 2.5% aqueous sodium carbonate solution‚ sodium nitrite‚ concentrated hydrochloric acid‚ N‚N-dimethylaniline‚ glacial acetic acid‚ 10% aqueous sodium hydroxide‚ saturated sodium chloride solution Apparatus 50 mL Erlemeyer flask‚ 250 mL beaker‚ test tube‚ hot plate‚ Buchner funnels Procedure In a 50 mL Elermenyer flask 1.2 g of sulfanilic acid and 12.5 mL of
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their green color and then turn red‚ yellow and brown. In observing my first set of maple leaves I noticed a high concentration of black spots in the yellow areas of the maple leaves. This observation brought mine to the question of‚ Do the dark brown spots appear mostly in the yellow area compared to the red areas on the leaves? The first set of Red Maple leaves I observed black spots that seemed to appear in higher amounts on the yellow areas rather than the red areas. My prediction was that the
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