PURIFICATION OF LACTATE DEHYDROGENASE FROM CHICKEN MUSCLE TISSUE Abstract The enzyme lactate dehydrogenase (LDH) catalyzes the last step of anaerobic glycolysis that is important for the normal function of the body. Purification of LDH is essential to understand its structure and function. The purpose of this experiment was to extract and purify LDH enzyme from chicken muscle tissue using a variety of various. Analytical methods such as activity and protein assay were employed to determine
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The Ponzi scheme Bernard L. Madoff entered a federal courtroom back in March of 2009 to admit that he had run a vast Ponzi scheme that robbed thousands of investors of their life savings. A Ponzi scheme is when potential investors are wooed with promises of usually large returns‚ usually attributed to the investment manager’s savvy skill‚ or some other “secret sauce”. Returns are repaid‚ at least for a time‚ out of the new investors principal‚ not from profits. This can continue as long as new
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plasmid DNA can replicate inside the transformed E.coli DH5α cells‚ only successful transformed cells can produce the protein that is resistance to kanamycin‚ this allows for the selection of successful transformed cells. 2. Overview of experiments DNA purification Maintain the PCR volume of 50µL-100 µL. Pipet the elution buffer in the center of the PureLink® Spin Column (PSC) and perform an incubation. Add B2 to PCR reaction. Add this sample to the PSC. Centrifuge the PSC. Replace the PSC into the
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Separation of Mixtures 9/11/11 * Introduction: A compound is a pure chemical substance consisting of two or more different chemical elements that can be separated into simpler substances by chemical reactions. Unlike a compound‚ a mixture is a material system made up by two or more different substances which are mixed together but are not combined chemically. There are two kinds of mixtures‚ homogeneous and heterogeneous. Homogeneous mixtures have the same composition and appearance throughout
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Chemical Separation Introduction: The objective of this experiment was to extract the pigments from spinach leaves‚ perform Thin Layer Chromatography (TLC) on the spinach leaf extract‚ and then determine the best solvent mixture to use to separate the pigments in the extract. The pigments are located inside the chloroplast walls in the cells of the spinach leaves. In order to obtain the pigments the cell walls must be broken down thus exposing the pigment containing chloroplasts. Upon
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Separation Techniques Aim: To separate a mixture of Iron filings‚ CaCO3‚ NaCl into their pure substances. Apparatus: • 2x 150ml beakers • 1x 150Ml Beaker • Magnet • Matches • Evaporating basin • Bunsen Burner • Water • Funnel • Retort stand • Funnel Paper • Sieve • Iron Filings • CaCO3 • NaCl • tripod • Gauze Mat • Cling Wrap • Saftey Goggles Method: 1. Gather Apparatus and the mixture of Iron‚ CaCO3 and NaCl. 2. Weigh beaker and mixture. 3. Use magnetic
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Chromatographic Separation of Sodium Fluorescein and Methylene Blue In this two-part lab the students will: • Learn the techniques associated with thin layer and column chromatography. • Determine the effects of the stationary phase in separating a mixture by chromatography. • Determine the effects of the mobile phase in separating a mixture by chromatography. • Conduct a separation using column chromatography. In the first week‚ the students will determine the best solvent system for
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p IB DIPLOMA PROGRAMME PROGRAMME DU DIPLÔME DU BI PROGRAMA DEL DIPLOMA DEL BI Chemistry Higher level and standard level Specimen paper 1s‚ 2s and 3s For first examinations in 2009 CONTENTS Chemistry higher level paper 1 specimen paper Chemistry higher level paper 1 specimen markscheme Chemistry higher level paper 2 specimen paper Chemistry higher level paper 2 specimen markscheme Chemistry higher level paper 3 specimen paper Chemistry higher level paper 3 specimen markscheme
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SCHEME OF WORK Centre: Course Code: Name of Course Horticulture Session length: 2.5 hours Name of tutor: Number of sessions: 6 Aims of course: To introduce students to horticulture giving them a basic understanding of plant life. Demonstrating and giving practical sessions on planting bulbs and seeds to develop the student’s skills and knowledge. Assumed knowledge: Basic skills read & write and ICT To enrich the learners’ experience and in line with Ofsted priorities‚ embed
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Objective: Separate and purify acetanilide from a mixture by recrystallization. Compare the melting points of crude and recrystallized acetanilide. Introduction : The most common method of purifying solid organic compounds is by recrystallization. In this technique‚ an impure solid compound is dissolved in a solvent and then allowed to slowly crystallize out as the solution cools. As the compound crystallizes from the solution‚ the molecules of the other compounds dissolved
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