The purpose of column chromatography is to separate one compound from another by the differing polarities in the compound. This technique is important in organic chemistry because certain experiments may require the separation of compounds in solution to be used for chemical synthesis and/or analysis of a particular desired product by isolating it. Fluorene is to be separated from 9-fluorenone using the technique of column chromatography. The success of separation and purity is determined with analyzation
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Making TLC Plates from Bulk TLC Silica Gels Many TLC users prefer to use pre-coated TLC plates‚ but others because of their special needs‚ or because they need special additives or a special thickness‚ coat their own TLC plates. This paper is a quick review of what is needed for the process. Initial instructions for plate coating: If reproducible plates are needed‚ then the user should invest in a coating device from Camag or Desaga (addresses below). The devices consist of a tray that holds
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Chromatography: Separating Mixtures Introduction: Magic marker inks are often mixtures of several compounds. Paper chromatography is a common method of separating various components of a mixture. After separation‚ you can observe the different colors that make up a particular color of magic marker ink. You can also calculate a ratio Rf‚ which compares how far each compound traveled to how far each solvent (substance that dissolves another substance) traveled during the experiment.
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CHEM 2204 Chromatography Lab by wyk.wong » Fri Jul 11‚ 2014 10:25 am Results and Calculations Rf values Rf=(Distance moved by the spot (cm))/(Distance moved by the solvent front (cm)) Toluene: Rf=2 cm/3.8 cm=0.53 (Fluorenone) Rf=1.1 cm/3.8 cm=0.29 (Fluorene) Hexane: Rf=1.8 cm/2.2 cm=0.82 (Fluorene) Rf=0 cm/2.2 cm=0 (Fluorene Table 1: Experimental IR peaks compared to literature IR peaks for fluorenone Functional group Experimental peak (cm-1) Literature peak (cm-1) C-H 3010.5 3013
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1ST SIX WEEKS PERIOD Unit A Chapter 1 Lesson 2 Classifying Plants Modeling Water Transport Material: * small sponge‚ * pie tin‚ * water‚ * glass‚ * red food coloring‚ * celery stalk. Procedure 1. Pour a cup of water into the pie tin and the glass. Add food coloring to the glass. 2. Have a student place the sponge in the pie tin and the celery stalk in the glass. 3. After 24 hours‚ ask students how the sponge and celery model water transport systems in
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Milgram experiment tells us about human and obedience. Humans are socially adapted to the society they live in and obedience is when a group humans follows the rule no matter wrong or right. Humans are usually obedient in most situations. That is due to teachings they receive. For example‚ when Hitler was killing groups of people‚ it was wrong; but the group of authority just listen to him and followed the rules. This situation was wrong and harmful but it was something that they just followed because
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Brad Birnbaum October 30‚ 2012 The Milgram Experiment Sociology 115 The Milgram experiment‚ a study based on a person’s obedience to an authority‚ was a series of social psychology experiments. These experiments measured the willingness of people to obey a person with authority. During the study‚ head figures instructed participants to perform acts that would normally conflict with their personal morality. Milgram’s experiments started shortly after the trial of German Nazi Adolf
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equivalence point is known then chemist can correctly determine the amount the concentration of the acid and the base. In this experiment acid-base titration will be used to determine the concentration of HCl at equilibrium when it reacts with the NaOH H+ (aq)+ Cl- (aq) + Na+ (aq) + OH- →H2O(l) + Na+ (aq)+ Cl- (aq) Procedure When performing this experiment one must first obtain and wear goggles. Next add 40mL of distilled water to a 100mL beaker‚ then add 5.00mL of HCl to the beaker
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Gas chromatography (GC) is a type of chromatography that uses a carrier gas as the mobile phase and a column as the stationary phase. The sample is injected into the instrument and is heated until the sample‚ which includes both analyte and solvent‚ boils. The analyte must have a relatively low boiling point in order to be to be pushed through the column along with the carrier gas‚ helium.1 The column used in the experiment was a non-polar Agilent HP-5 column (specific
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The Milgram Experiment Milgram experiment was conducted at 1962 by Psychologist Stanley Milgram at Yale University. This experiment focused on how people will behave when their moral senses are conflicting with the authority. This experiment measured if people will obey authority or stand up what they believe for when their morals are challenged by a person with a greater social figure. These people who participated in the experiment were males in ages between twenty and forty. The volunteers were
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