Affinity chromatography technique is used to separate proteins found in a mixture of solution. Affinity chromatography uses the strong interaction between a given protein and its corresponding molecule. In today’s lab‚ affinity chromatography was used to purify L-lactate dehydrogenase‚ which contains histidine-tagged protein. The histidine- tagged protein forms a strong interaction with the Ni-NTA column due to the presence of nickel ions. Varying concentration of imidazole was added to the column
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Acids by Cation Exchange Chromatography Introduction and Purpose: Amino acids are small biomolecules that have a carboxylic acid backbone in common‚ as well as an amino group attached to a saturated carbon. There are many amino acids‚ but there are 20 most commonly know amino acids. Amino acids are the fundamenta building blocks of other biomolecules like proteins and ezymes (Davidson‚ 2015). This experiment examined a mixture of 3 amino acids. The purpose of this experiment was to identify amino acids
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Austin Vandermey GS-1140 April 4th‚ 2015 Experiment We as a society have one big problem on our hands. This problem is sleep deprivation because there are not enough hours in the day to get stuff done. Between work‚ school‚ family‚ and leisure it seems like we do not have enough time to get are daily tasks done let alone have time for sleep. It is recommended by the U.S Department of Health that people need a solid eight hours of sleep to maintain good health. I don’t know about you
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EXPERIMENT 5 REDOX TITRATION: TITRATION USING SODIUM THIOSULPHATE Objectives 1. 2. To prepare a standard solution of potassium iodate for use to determine the concentration of sodium thiosulphate solution accurately. To acquire the proper techniques of carrying out a titration. Introduction Redox titrations using sodium thiosulphate as a reducing agent is known as iodometric titration since it is used specifically to titrate iodine. The reaction involved is: I2 + 2Na2S2O3 I2 + 2S2O322NaI + Na2S4O6
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Experiment : 1 Tittle : Preparation of bis(acetylacetonato)copper(II) complex Objective : To synthesis the bis(acetylacetonato)copper(II) complex Introduction : A complex ion is usually form with high charge density metal ion as a central and formation of coordinate covalent bond (dative bond) with high electron molecules or ions. These molecules or ions are functioning as ligands (electrophile in organic compounds). They are easily attracted by electrophile (electron deficiency
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Arlind Mara Paper Towel Lab Report AP Biology 15 September 2011 Which Towel is The Quicker-Picker Upper! To determine which of the most popular paper towel brands such as Bounty and Marcal would be the Quicker-Picker-Upper I conducted the following experiment during which I dipped 6in X 6in squares of paper towels into 100mL and measured the amount of water absorbed by the towels in 20 seconds. After averaging all 3 trials for the paper towel brands that I tested I arrived to the conclusion
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Task 3: How could Thin Layer Chromatography (TLC) have been used to prepare a pure sample of aspirin? The method of using thin layer chromatography is easy‚ quick and relatively cheap to carry out this makes it ideal for preparing pure samples of aspirin. Equipment Required: • Thin Layer Chromatography Paper • Ruler • Pencil • Solvent • Beaker • Micro Capillary • Access to a fume cupboard • UV light • Clingfilm • Individuals sample of aspirin • Pure sample of aspirin • Sample of salicylic acid Method:
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Tittle: Separation of leaves pigment. Introduction The photosynthetic pigment is used to absorb light during the process of photosynthesis. There are five pigments usually found in spinach leaves: Carotene (yellow)‚ Phaeophytin (yellow-grey)‚ Xanthophyll (yellow-brown)‚ Chlorophyll a (blue-green)‚ Chlorophyll b(green). Photosynthetic pigment is located in the chloroplast of the leaf. The function of chlorophyll a and chlorophyll b is to trap red and blue violet light when they carry out photosynthesis
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Mobile liquid phase is always passed stationary phase during chromatography. Developed TLC plate is then observed under short wave ultraviolet light‚ where most TLC plates contain fluorescent mixed with silica gel that allow to glow green color. Compounds will absorb the UV light and appear as dark spot against the green color TLC plate. In a column chromatography‚ cotton and sand is placed in purpose of holding silica gel and prevent leakage of adsorbent particles
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Experiment 4 – Liquid Phase Chromatography I. Objectives This experiment’s goal is to explore one-dimensional and two-dimensional paper chromatography. II. Schematic Diagram of the Procedure PAPER CHROMATOGRAPHY Wash leaves‚ cut them into smaller pieces; in a mortar macerate them in circular motion Add 8mL ethyl alcohol to extract pigments‚ continue macerating until finely grounded Transfer extract to evaporating dish‚ allow to conc‚ don’t let extract to dry out Concentration
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