Thin Layer Chromatography Introduction Thin Layer Chromatography or TLC is a technique used as a separation and identification technique. There are many forms of chromatography‚ but one thing that remains constant throughout all of the types of chromatography is that there is a stationary phase and a mobile phase. In the case of TLC the stationary phase is the silica gel on the TLC tray. Procedure Chromatograph method is a method of separating mixtures of two or more compounds. Two phases
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________ Name: Loren B. Matulac Date : November 09‚ 2013 Yr. & Section: IV- Perseverance Teacher: Mrs. Pacita J. Yapsangco “Magnetic Field of a Coil” Experiment 2.5 I. Objectives: To produce a strong magnetic field just by looping the wire into coils II. Materials: 6 V or 9 V batteries 50 cm of bare 12- gauge copper wire Stiff cardboard and scissors Wooden dowel (about 15 cm long x 4 cm
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The Stanford Prison Experiment was created by a psychology teacher named Phillip Zimbardo. There was 9 prisoners and 9 guards. Those that were chosen were arrested one morning and taken to the station where they were blindfolded. An ad was put in the local paper asking for volunteers for this project. This experiment was to see the psychological effects of being in prison. After reviewing over 70 applicants‚ they narrowed it down to twenty-four candidates. The candidates were college students from
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The milgram experiment. The three people involved were: the one running the experiment‚ the subject of the experiment a volunteer‚ and a person pretending to be a volunteer. These three persons fill three distinct roles: the Experimenter an authoritative role‚ the Teacher a role intended to obey the orders of the Experimenter‚ and the Learner the recipient of stimulus from the Teacher. The subject and the actor both drew slips of paper to determine their roles‚ but unknown to the subject‚ both slips
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CHROMATOGRAPHY _TOPIC_ : 7.2 _RESEARCH QUESTION:_ What is chromatography? How many pigments are there in a plant? How the different pigments in a plant can be separated? _APPARATUS_ : Please refer to the handout _MATERIAL_ : Please refer to the handout _METHOD_ : Please refer to the handout _DATA COLLECTION_: Coloured band Pigment front distance (± 0.05cm) Solvent front distance (± 0.05cm) Green 11.7 13.0 Yellow 12.5 13.0 _DATA PROCESSING_: Coloured band Pigment
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Background Information and Research Paper chromatography uses selective adsorption on a strip of paper. It is used for separating and identifying mixtures that are or can be colored‚ especially pigments. This can also be used in secondary or primary colors in ink experiments. Is it used for Contaminants in rainwater‚ Analysis of narcotics‚ and Detection of substances in urine. Purpose • The purpose of this lab is to see the separation of dye on the candy. Data and Observations Marker Color
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Performance Liquid Chromatography (HPLC) Instructor: Dr. Hüseyin BOZKURT High Performance Liquid Chromatography (HPLC) is one mode of chromatography‚ the most widely used analytical technique. Chromatographic processes can be defined as separation techniques involving mass-transfer between stationary and mobile phases. Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is referred to as high performance liquid chromatography (HPLC).
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BIOL 1F90 Experiment #2 Aerobic and Anaerobic Metabolism Caleb Coolen 5407754 Lab Section 21 Experiment Performed: October 10‚ 2013 Lab Partners: John Elgersma‚ Sandra-Lynn Plourde & Taylor Spratt Abstract: Materials and Methods: Methods and materials used in this experiment can be found in the BIOL 1F90 Laboratory Manual (Martin‚ 2013)‚ Experiment # 1‚ pages 2-5. Results: Table 1: Culture Sample Chromatography Rf Values Sample of Spot Distance from Origin (mm) Solvent Front
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Experiment 2: Dehydration of an Alcohol: Distillation and Gas Chromatography Preparation of Methylcyclohexenes Purpose: The basic purpose of this experiment is to carry out the dehydration of an alcohol and isolate the reaction products by distillation. Gas Chromatography will be utilized to analyze the reaction mixture. Table of Reagents: Compound (g) Molecular Weight (g/mol) Grams (g) Moles 6 mL of 2-methylcyclohexanol (C7H14O) 114.19 g/mol 6 mL x 0.943g = 5.66 g 1 mL 5.66 g x
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Corey Davis H 312 TR 12 Noon Writing Assignment #1 April 17‚ 2011 The Tuskegee Syphilis Experiment was started in the early 1930’s and continued on for over 40 years causing a great deal of physical and emotional health problems to thousands of black men and their families in Macon County‚ Georgia. Beneficence‚ according to The Belmont Report states‚ “Research involving human subjects should do no intentional harm‚ while maximizing possible benefits and minimizing possible harms‚ both to
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