Lux G experiment is performed at the University of California San Diego on May 21st until June 4th of 2018 by Gloria Karem. This experiment is based on the purification of LuxG by using multiple purification and lysing methods: Brad Ford Assay‚ SDS-PAGE‚ affinity purification‚ lysozyme treatment‚ nickel column‚ sonication‚ and flavin reeducate activity assay. His-tagged protein is expressed in E. coli from pGhis‚ with the T7 RNA lac repressor induction system.5 Sonication is used‚ it’s why bacteria
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EXPERIMENT 3: ISENTROPIC EXPANSION PROCESS Objective In order to demonstrate the isentropic expansion process. Introduction Isentropic means no change in entropy. Entropy is a thermodynamic property that is the measure of a system’s thermal energy per unit temperature that is unavailable for doing useful work. In simple terms‚ the measure of the level of disorder in a closed but changing system‚ a system in which energy can only be transferred in one direction from an ordered state to a disordered
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photosynthesis. Problem What is the relationship between light intensity and the rate of photosynthesis? Pre-Lab Discussion Read the entire investigation. Then‚ work with a partner to answer the following questions. 1. What are the variables in this experiment? Identify the manipulated and responding variables and two controlled variables. The manipulated variable is light intensity. The responding variable is pH. Controlled variables include the initial pH‚ the size of the plant sprigs‚ and time
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In order to purify CelB2 protein bound to maltose-binding-protein‚ amylose affinity chromatography was performed. The amylose resin present in 20% ethanol was first diluted by adding 10 mL of 20 mM TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA and centrifuged at 700 rpm for 5 minutes. After decanting the buffer‚ another 10 mL of TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA was added to this resin solution and centrifuged at 700 rpm for another 5 minutes to further dilute the ethanol concentration in the resin
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EXPERIMENT 10 POTENTIOMETRIC REDOX TITRATION BAUTISTA ‚ Anne Judith LARIN‚ Michelle V. OBJECTIVES At the end of the experiment‚ the student should be able to: 1.Construct a potentiometric redox titration curve 2.Determine the % Fe in a sample by potentiometric redox titration INTRODUCTION Potentiometry definition indicator electrode reference electrode Potentiometric titration applications general procedure similarities with redox titration INTRODUCTION Determining the endpoint
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Calorimetry Experiment Purpose: The objective of this lab is to determine the enthalpy change for NaOH(aq) + HCl(aq) NaCl (aq) + H2O(l) Procedure: Before measuring the enthalpy of acid base neutralization‚ my partner Brian and I determined a calorimeter constant‚ using a homemade polystyrene calorimeter. With the following formula and data: qhot= cm (Tf-Ti) qcold=cm(Tf-Ti) SYSTEM DATA SURROUNDINGS DATA Water cold Mass: 50mL Water hot Mass: 50mL C=4.18 C=4.18 Ti=20 C Ti=31
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The Manland Experiment 1. The Manland experiment lasted only one weekend in one store. The marketing advantages and disadvantages of expanding it to other stores? Do you think IKEA should do more with this idea? Why or why not? The advantage was this was an attempt to continue IKEA’s image as family-friendly with the goal to appeal to all men as an environment offering a rewarding shopping experience. The area was set up for men to play video games or watch sports while their wives or girlfriends
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EXPERIMENT 201: WORK‚ ENERGY AND POWER John Michael A. Ramos‚ Phy11l/A5 Abstract The essential conditions to be satisfied for work to be done are: Some force must act on the object. The point of application of force must move in the direction of force. W = F x s. SI unit of work is joule. Energy is the capacity to do work. The two types of mechanical energy are kinetic energy and potential energy. Kinetic energy is the energy possessed by an object by virtue of its motion. Potential energy is
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The effect of alcohol concentration on the cell membrane Abstract: In this experiment I found out that as the concentration of the alcohol was increased so did the colour intensity of the solution. This is because the higher concentration of ethanol‚ results in more damage done to the cell membrane‚ resulting in leakage of red pigment from the cell. If the membrane is damaged more‚ more red pigment will leak out of the membrane and into the ethanol. Hypothesis: The higher the concentration
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of the consequences of excessive drinking. After connecting with her‚ I wanted to know if alcohol concentration in spirits would affect the cells in the body. Thus‚ I decided to test my theory by adapting a class beetroot experiment to my investigation. The aim of this experiment is to investigate the effect of alcohol concentration on a biotic cell membrane. The human body cannot live long with damaged cells because they are the building blocks of life. Cell membranes are phospholipid bilayers meaning
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