of SINGAPORE Chemical Engineering Process Laboratory II Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein
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Data and Conclusions: The purpose of this experiment was to learn how to use distillation and gas chromatography to separate and identify different compounds from a given mixture. There are several kinds of distillation methods. However‚ the method that we used in this experiment was fractional distillation. This method is used when trying to separate two different volatile compounds whose boiling points differ by 40-50°C or more. If the boiling points are too close‚ this method
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Liquid Chromatography – Laboratory #18 Introduction: We are using liquid chromatography to separate the colored substances in grape-flavored drinks. We separate the component dyes‚ and then we separate the flavorings and citric acids. Background: Chromatography is a process that is used to separate a substance into its component parts. The separation occurs between the stationary and moving phase of the lab. The moving phase consists of a fluid and the stationary phase consists of a solid
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Experiment 2: IDENTIFICATION OF UNKNOWNS BY TLC AND MP IN COMBINATION Introduction: Thin layer chromatography (TLC) is one of the most valuable techniques in organic chemistry. This is a best method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that is stationary and one that is moving or mobile. Chromatography works on the principle that different compounds will have different
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favorite candies? Chromatography can solve that for you. Chromatography is a separation technique used by scientists for separating both organic and inorganic compounds. There are four different types of chromatography: thin layer‚ liquid‚ gas‚ and paper‚ but for this lab paper chromatography will be used. Who invented chromatography? A Russian botanist named Mikhail Semyonovich Tsvet invented chromatography in 1901 while doing research on plant pigments. Why is chromatography so important? This
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The Tuskegee experiment was a mind blowing experiment conducted by the Public Health Service (PHS) which lasted forty-years. It took place between 1932 and 1972 in Tuskegee‚ Alabama. This experiment affected many African-American males who were basically used as human “guinea pigs” in order to follow the movement of Syphilis. According to Harry Reasoner‚ “they used human beings as laboratory animals in a long and inefficient study of how long it takes syphilis to kill someone.” (Harry Reasoner).
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What will happen when you put a drop of water on wax paper? I think that it will just sit there and do nothing. First I put a drop of water on the paper Second I watched it to see what it will do Finally I wrote down that it just stayed still My hypothesis is correct What will happen when you move the water around with a toothpick? I think it will go flat and dissolve in the in the wax paper. First I picked up the toothpick Second I moved the water around with the toothpick Finally
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Anionic Exchange Ion Chromatography to Detect the Anions Concentrations in the water Samples Narendra Boppana‚ Nasser Ali‚ Martin Chavez‚ Sear Introduction: Ion Exchange Chromatography is a process where the ionic exchange occurs in between the stationary phase and the sample in the mobile phase‚ by using this ionic exchange phenomenon it is easy to separate the compounds. Ionic exchange depends on the electrical properties
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Orange and Lemon Battery Experiment Darren Chymbeline Pineda‚ Jade Salentes‚ Karryle Olarte‚ Kathlene Malunao‚ Felix Villarente VII-6 Mahinahon Sta. Elena High School‚ Marikina City Summary All people need energy in various forms such as heat‚ light‚ sound‚ etc. to sustain their everyday lives. Although these various forms of energy are equally needed by people‚ not all of them are always available‚ especially the energy needed to power up their main appliances which is electricity. Due to the inevitability
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of Vitamin C contained. The concentration of Vitamin C contained of these fruit juices were identified by using titration. Based on the results‚ the concentration of Vitamin C contained in these 3 fruit juices were obtained. The conclusion of the experiment is that different fruit juices contain different concentration of Vitamin C which is important in our daily lives. CHAPTER 1 1.0 INTRODUCTION 1.1 THEORY/LITERATURE Vitamin C is an essential nutrient in humans as it functions as a cofactor in several
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