LAB 9: DATE: 25TH January‚ 2011. FORM CLASS: L6 3 SUBJECT: Biology TITLE: Enzymes AIM: To investigate the effect of temperature on the enzyme lipase INTRODUCTION: The phenomenon of catalysis makes possible biochemical reactions necessary for all life processes. Catalysis is defined as the acceleration of a chemical reaction by some substance which itself undergoes no permanent chemical change. The catalysts of biochemical reactions are enzymes and are responsible for bringing about almost
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Introduction This practical experiment was performed in an attempt to observe how enzymes (starch phosphorylase in particular) are affected by varying its temperature before introducing it to the substrate it will be reacting with. A catalyst (enzyme) is a substance that changes the rate of a reaction; for a reaction to take place at all‚ the enzyme must first come into contact with the substrate. Enzymes are subject to a number of factors which effect how fast they can cause a reaction with a substrate;
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Investigating the effect of temperature on plant cell membranes The purpose of this activity is: • to practise experimental and investigative skills • to investigate the effect of temperature on cell membrane structures Procedure SAFETY: Always carry scalpels clasped to a tile and with the tip pointing away from you. Beetroot cells contain pigments called betalains that give the tissue its dark purple-red colour. The pigment is contained in the cell vacuole. Investigation
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Introduction The aim of this experiment was to determine the effect of caffeine on resting body temperature. This has to do with homeostasis‚ which is the maintenance of equilibrium. In other words‚ it is a stable body state. Homeostasis refers to the process of keeping the internal body environment in a steady state‚ when the external environment is changed (Homeostasis 2). Humans are warm-blooded creatures that generate body heat internally and maintain body temperatures at a fairly constant level
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Introduction To determine the effects of stressful experimental treatments on living membranes we are going to examine how fresh beets roots react when they are exposed to different temperatures. Membranes are an important feature of plant cells and they act as a barrier that separates the interior of the cell from the external environment (Campbell 133). They organize specific chemicals and reactions into specific compartments within the cell. Generally‚ cell membranes consist of phospholipids
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Effects of Temperature and pH on Catalase Activity INTRODUCTION Enzymes are organic catalysts that spur metabolic reactions. The presence of an enzyme within a cell is essential in order for any sort of reaction to take place. All enzymes are complex proteins that act in an organism’s closely controlled internal environment. In such a homeostatic environment‚ the temperature and the pH (concentration of hydrogen ions)‚ remain within a fairly narrow range. Extreme variations in pH and temperature
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The Effect of Temperature on Blow-fly Larvae INTRODUCTION Calliphoridae- comonly known as a blowfly‚ are living organisms which usually are the first insects to colonise a body after death. The blowfly larvae are very useful in forensic entomology. The oldest larvae give an approximate time of death of body. Family: Calliphoridae Order: Diptera Name: Calliphora vicina Aim: To check the influence of temperature of environment on living functions and activity of Blow-fly larvae.
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Introduction For this experiment‚ we are going to determine the effect of temperature on solubility‚ to be done in a chemical by dissolving a solute in a definite amount of solution which is saturated. Specifically‚ the goal of this experiment is to prepare a saturated solution of Na2C2O4 in water at different temperatures‚ determine the effect of temperature in solubility‚ and to apply Le Chatelier’s Principle. We can do all this by simply titrating a certain amount of standard KMnO4‚ and measuring
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Effect of temperature on enzyme activity In this experiment… Independent variable: Temperature of the amylase Dependent variable: Enzyme activity which is measured by the time for disappearance of starch Controlled variables: Volume of amylase; volume of starch solution; concentration of amylase; concentration of starch solution Prediction of results i) At low temperature‚ the rate of amylase activity is very low. ii) At optimum (=best) temperature‚ the rate of amylase activity is the
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The Decomposition of Potassium Chlorate Hypothesis: Potassium chlorate decomposes into potassium chloride and oxygen gas‚ which means that when the glowing splint is inserted into the test tube‚ the flame will have enough fuel to fully reignite the splint. When the manganese dioxide is added‚ this will increase the rate of the production of oxygen in the reaction‚ without affecting the state of the manganese oxide. Materials: Test tube Retort stand Bunsen burner Scoopula Flint sparker 2cm of potassium
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