The Effects of varying p-Nitrophenyl Phosphate concentrations on Alkaline Phosphatase Activity Nathan Overholt Louis Settembrino March 5‚ 2002 Abstract: The enzyme Alkaline Phosphatase was set up in solutions of varying p-nitrophenyl phosphate concentrations. A fixed time assay was used to find the Michaelis constant and Vmax. All solutions were incubated at 30 C° for 30 mins. Absorbances were measured at 405 nm‚ and the Km was found to be 0.002 M (pNPP) and the Vmax to be 0.433 A/min. Introduction:
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EFFECT OF ROOM TEMPERATURE ON THE BURN-RATE OF A CANDLE BURNS Have you ever wondered whether a candle burns more quickly on a hot day‚ than on a cold day? Or is it the other way around? This science fair project was carried out to compare how quickly a candle will burn at different room temperatures. The experiment was done by measuring how much of a candle will melt in 30 minutes when placed in an environment with surrounding temperatures of 20°C‚ 25°C and 30°C. Hypothesis A candle in a colder
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Hampton | | |Restriction Enzymes: | |A study in Reactions and Mapping | | | |November 7‚ 2008 | ABSTRACT This experiment will study the manner in
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to learn... when is it that we will understand that the well-being of the environment and our survival are intricately woven into each other. Good morning respected teachers and my dear friends ...today I Nikita am going to speak about how human activities are affecting flora and fauna? We are facing a serious crisis in biodiversity‚ the variety of animals‚ plants and their habitats. We humans are part of this biological diversity and depend on it in many ways. The rate that animal and plant species
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Enzymes Lab Report Inroduction In this lab we explore an enzymes activity and how it can be affected by changes to its environment. An enzyme is a protein and is a catalyst to chemical reactions. It helps accelerate reactions by lowering the activation energy‚ which is needed for reactions in cells to progress at a higher rate. Activation energy is the minimum amount of energy needed for a chemical reaction to occur‚ yielding products from a given set of reactants. (Unit 7: Enzymes lab) Products
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BABS1201 Group Enzymes Project Group Protocol Including Equipment and Reagents List Lab Day: Wednesday Lab Time: 10am – 1pm Are you in Lab G20 (furthest from BSB Student Office) or Lab G21 (closest to BSB Student Office)?: Demonstrator Name: Daniel Winters Names of Group Members: Johnny Nguyen‚ Therese Pham‚ Linda Tang Name of Enzyme You are Investigating: Amylase Brief Background: Amylase is a digestive enzyme‚ produced mainly by the salivary glands and the pancreas‚ to break down
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The effect of time on enzyme reaction. Abstract: In this lab investigation we will observe how the amount of hydrogen peroxide is affected by catalase over time. The enzyme was added to 10 mL’s of hydrogen peroxide and observed over time to determine the relation between time and enzyme activity. The hypothesis stated that as time increased substrate would decrease. Therefore I predicted that at 60 seconds‚ there would be the least amount of H2O2. The enzyme activity mirrored my predictions
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Investigation on the effects of Temperature‚ pH levels‚ and Enzyme Concentration on the reaction rate of the Decomposition of Hydrogen Peroxide by Catalase The purpose of this investigation is to figure out how temperature‚ pH‚ and enzyme concentration affect the reaction rate of an enzyme. It’s important to understand how certain factors affect enzymes because of their crucial role in the metabolic processes of life. Enzymes lower the activation energy necessary for a chemical reaction to
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The effects of human activity on ecosystems and biodiversity Agricultural Practice Biodiversity is a measure of the diversity among organisms in different ecosystems. "Diversity" in this definition includes diversity within a species and among species‚ and among ecosystems.’ Monoculture is when a farmer produces or grows one single crop over a wide area. The advantages to the farmer of monoculture are‚ “the reduced plant competition for nutrients‚ space and solar radiation‚ control of unprofitable
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Ah Seung Chong Molecular Biology CTW: Enzyme Kinetic Dr. Cruz 07/22/2010 Enzyme kinetics Introduction Enzymes are biological catalysts or assistants‚ without enzyme many of important processes of life could not happen. Most of enzymes are proteins that help speed up chemical reactions by lowering amount of activation energy needed for the reaction1. Enzymes are usually highly selective‚ only bind to specific substrate and convert it to product at a particular rate1. The rate of the reaction
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