Enzymes All enzymes are globular proteins and round in shape They have the suffix "-ase" Intracellular enzymes are found inside the cell Extracellular enzymes act outside the cell (e.g. digestive enzymes) Enzymes are catalysts → speed up chemical reactions Reduce activation energy required to start a reaction between molecules Substrates (reactants) are converted into products Reaction may not take place in absence of enzymes (each enzyme has a specific catalytic action) Enzymes catalyse
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This experiment shows the effects of changing the pH level has on Catalase. As predicted‚ the farther away the pH levels got from the optimum pH (7.2)‚ the lower the reaction rate. At a pH of 7.2‚ the foam of the reaction measures 6cm. At a ph of 3 it measures 2.5 cm‚ at a pH of 5 it measures 2.75 cm‚ at ph 9 it measures 2.3 and at 11cm it measures 2cm. pH measures the hydrogen ion concentration of a substrate. By changing the pH of the catalase‚ the enzyme was denatured. Denaturing is the result
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Enzyme as protein Dr.Samina Haq Quantitative and qualitative test for protein and amino acids • 1. 2. 3. 4. 5. 6. Qualitative test Ninhydrin test Biuret test Xanthoproteic test Millons test Hopkins-cole test Nitroprusside test Quantitative test 1. 2. 3. Spectrophotometric assay Protein shows maximum absorbance at 280nm due to presence of tyrosine and tryptophane. Biuret test shows 540nm Lowry test shows 750nm Ninhydrin Test • Amino acid containing a free
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Enzymes Enzymes are the sparks that start the essential chemical reactions our bodies need to live. They are necessary for digesting food‚ for stimulating the brain‚ for providing cellular energy‚ and for repairing all tissues‚ organs‚ and cells. There are three types of enzymes: metabolic enzymes‚ digestive enzymes‚ and food enzymes. Metabolic enzymes catalyse‚ or spark‚ the reactions within the cells. The body’s organs‚ tissues and cells are run by metabolic enzymes. Without them our bodies
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Enzymes are biological molecules (proteins) that act as catalysts and help complex reactions occur everywhere in life. Enzymes are catalyst for biochemical reactions; Enzymes lower the activation ATP so a reaction can begin over the energy barriers. In this lab‚ a discussion of the enzymes reaction to heat will be addressed. Does heat speed up the enzyme reaction? The prediction is as more heat is applied more reactions will occur then at some point the heat will denature the enzyme as it reaches
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Enzyme Activity Lab Hypothesis: 1] Are enzymes reusable? Yes‚ enzymes are reusable. 2] Which of the following do you think contain catalase? [Manganese dioxide‚ chicken meat‚ apple‚ carrot‚ potato‚ or chicken liver] I think that the manganese dioxide‚ apple‚ and carrot contain the catalase. 3] Would grinding the materials affect enzyme activity? I think that grinding the materials will increase enzyme activity. 4] Would temperature affect enzyme activity? I think that
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Stoichiometry of a Precipitation Reaction Submitted by Hendy Zelishovsky Date Submitted: 4/26/2012 Date Performed: 4/25/2012 Lab Section: Chem-180-DL1 Course Instructor: Dr. Cynthia Spencer Purpose The purpose of this experiment is to use stoichiometry
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Enzymes are organic catalysts‚ usually proteins that speed up metabolic reactions. They lower the amount of energy needed for reactions to progress in cells. In enzymatic activity‚ the molecules at the beginning are called substrates. Lactose metabolism is when lactose is destroyed‚ maintained or produced. For instance‚ being lactose intolerance that’s where lactose is destroyed. Metal cofactors in enzyme activity are required to function properly. The Effect of Temperature on Enzymatic Activity:
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Enzymes play a vital role in helping our body function. They act as biological catalysts and help speed up reactions that would otherwise take long periods of time to naturally occur. Enzymes help lower the activation energy required for the reactants to reach the transitional state from which then they can form products. However‚ enzymes do not change the free energy of the reaction. Enzyme’s ability to catalyze reactions comes from the shape of the active site on the enzyme. Enzymes are hyper-specific
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Factors that affect how the PH changes during a titration experiment. Concentration of the alkali Having a higher concentration of alkali will mean that there will be more molecules closer together for the acid to collide with. This will speed up the reaction as collision theory suggests that molecules have to collide to react and if there are more molecules to collide with the reaction will happen faster as there is a higher chance of a collision. Volume of alkali An increase in volume would mean
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