Red Cabbage Indicator Aim – To create a pH indicator out of a red cabbage solution and to construct a basic pH scale to determine the pH of unknown solutions. Materials - • red cabbage leaves • 250 mL beaker • hotplate or Bunsen burner‚ tripod‚ gauze mat and bench mat • 10 test-tubes – equal size • test-tube rack Methods - Part A: Making the indicator 1. Tear up one or two red cabbage leaves‚ and place them in the beaker with enough water so that the cabbage is just covered. 2. Heat the beaker
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The effect of time on enzyme reaction. Abstract: In this lab investigation we will observe how the amount of hydrogen peroxide is affected by catalase over time. The enzyme was added to 10 mL’s of hydrogen peroxide and observed over time to determine the relation between time and enzyme activity. The hypothesis stated that as time increased substrate would decrease. Therefore I predicted that at 60 seconds‚ there would be the least amount of H2O2. The enzyme activity mirrored my predictions
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Enzyme Activators and Inhibitors Lucia House AP Biology‚ Block 4 Mr. Trice October 18‚ 2012 Introduction: Metabolism is the totality of all of an organism’s chemical reactions. Chemical reactions occur due to enzymes‚ a substance which acts as a catalyst in driving chemical reactions in order to produce a desired product (Campbell and Reece‚ 2002). A catalyst is usually a protein; however‚ some catalytic molecules counter this generalization. A discovery made in the early nineteen- nineties
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temperature on reactions between the enzyme catalase found in animal tissue with the substrate H2O2. The hypothesis stated that an increase in the temperature of the substrate would create a subsequent increase in the rate of reaction between the enzyme and the substrate. This hypothesis was tested by immersing 1cm cubes of animal tissue (sheep liver) which contained the enzyme catalase into the substrate (H2O2 ) mixed with detergent which foamed showing a visual display of the reaction. After 10 seconds
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The Effects of Varying pH on Enzyme Activity A lab was conducted to test different pH balances on an enzyme. Introduction: Enzymes are protein catalysts that speed up a chemical reaction without being consumed in the process. Enzymes are three-dimensional structures that consist of one or more polypeptide chains. The polypeptide chains form an active site (where a substrate will fit into). Enzyme molecules are folded into a very specific shape held together by the different forces of attraction
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Enzymes are biological catalysts that speed up chemical reactions by lowering the amount of activation energy needed to start the reaction and let the reaction occur at temperatures found in living cells. The way that enzymes do this is explained with the lock and key hypothesis. This hypothesis says enzymes have a specific shape called the active site which is different between different enzymes. Molecules called the substrate that participates in the reaction also have a specific shape that can
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The experimental results were different from the hypothesis because while the enzyme appeared to not work as well‚ I expected a more significant change. Most of the time‚ there was only a millimeter of difference of the foam between the two samples while I expected a greater difference such as 10 millimeters. Enzymes speed up chemical reactions and at the active site‚ a substrate can be broken down or two substrates can form a larger molecule. Hydrogen peroxide is broken down by peroxidase into water
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Introduction: Enzymes are a substance produced by a living organism that acts as a catalyst to activate a specific reaction. The purpose of this experiment was to figure out if the temperature of the reaction would rise‚ will the absorption rise as well. Reactions use energy‚ If there is energy than heat occurs. The Hypothesis that was figured out was‚ If the temperature rises‚ then the absorption will also go up. The Independent variable that was tested was temperature. The dependent variable that
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Maintaining a Balance – Enzyme Activity 1.P.1 Identify data sources‚ plan‚ choose equipment and perform a FHI to test the effect; increased temperature‚ change in pH and change in substrate concentrations on the activity on the activity of a named enzyme Introduction: Enzymes are catalysts which aid in process of chemical reactions within living organisms. Enzymes increase the rate of biochemical reactions without causing any permanent chemical change to itself. A substrate is the chemical
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An experiment to investigate the effect of temperature on the rate of reaction of the Enzyme Trypsin. Aim: This investigation was on the effect temperature has on the rate that the enzyme trypsin hydrolyses its substrate‚ a protein found in milk (casein). This investigation was conducted under controlled conditions‚ the temperature being the changeable variable. Trypsin and its substrate (powdered milk which is a source of the protein casein) were heated in a water bath. The contents of the two
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