BIOCHEMISTRY 304 Enzyme Kinetic Sample Problems #1 September 2004 1 Given the reaction k1 kp E + S ES E + P k-1 where k1 = 1 x 107 M-1 sec-1 k-1 = 1 x 102 sec-1‚ and kp = 3 x 102 sec-1 a) Calculate Ks b) Calculate Km (a) k-1 1 x 102 sec-1 Ks = k1 = 1 x 107 M-1 sec-1 = 1 x 10-5 M (b) k-1 + kp (1 x 102 sec-1) + (3 x 102 sec-1)
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Nonbiological Material on the Reaction to a Changing pH Level This experiment was conducted to learn exactly how biological material responds to rising or dropping pH levels in comparison to how nonbiological material does. The dependent variable in this experiment was how the different materials react‚ and the independent variable was the type of material‚ biological or nonbiological. Tap water was the selected nonbiological material‚ and the generally known pH level for water is 7‚ or neutral
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introduction to enzymes Enzymes are the foundation of energy and the life force in all living things. They are responsible for building‚ detoxifying‚ and healing the body. They are also the force that allows your body to digest and absorb food. Enzymes also regulate tens of thousands of other biochemical functions that take place in the body every day. Without enzymes‚ seeds would not sprout‚ fruit would not ripen‚ leaves would not change color‚ and life would not exist. Therefore‚ the study of enzymes has
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Experiment 3: Soil pH measurement Introduction Aims The aims of the experiment were to determine the pH of a variety of soils which included sedentary‚ 3:2‚ sand‚ organic matter with the means of a pH meter at various soil water ratios and with or without calcium chloride. Method and Materials Samples of sedentary soil‚ 3:2 soil‚ sand‚ organic matter and compost‚ 100ml vials(x12)‚ bottle of distilled water‚ analytical balance‚ pH meter‚ 2 buffer solution of known pH‚ 0.25M calcium
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Enzyme Immobilization Methods Covalent Binding: Covalent binding is a conventional method for immobilization; it can be achieved by direct attachment with the enzyme and the material through the covalent linkage [37]. The covalent linkage is strong and stable and the support material of enzymes includes polyacrylamide‚ porous glass‚ agarose and porous silica [38]. Covalent method of immobilization is mainly used when a reaction process does not require enzyme in the product‚ this is the criteria
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Enzymes and their importance in plants and animals (25 marks) Enzymes are biological catalysts‚ which accelerate the speed of chemical reactions in the body without being used up or changed in the process. Animals and plants contain enzymes which help break down fats‚ carbohydrates and proteins into smaller molecules the cells can use to get energy and carry out the processes that allow the plant or animal to survive. Without enzymes‚ most physiological processes would not take place. Hundreds of
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Dehydrogenase catalyzed reaction. 6. To determine the effect of heat on a Xanthine Dehydrogenase catalyzed reaction. Introduction: Enzymes are specialized biologically made proteins that have catalytic functions that are essential for the maintenance and activity of life (Amano 2007). All living cells have enzymes but they are different‚ they depend on the concentration of enzymes that are found in the organelles. A catalyst is a substance that speeds up a chemical reaction but remains unchanged
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In this enzyme lab‚ (insert creative name)‚ the enzyme catalase was observed under varying conditions in order to interpret what prohibits and inhibits the functioning of certain enzymes in chemical reactions. Enzymes aid in chemical reactions by speeding up the time it takes for the reaction to occur‚ without getting “used up” throughout the process. Catalase‚ the specific enzyme used in this lab‚ is a protein abundant in the liver and red blood cells. It enhances the breakdown of hydrogen peroxide
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Immobilization of Enzymes And Their Applications By‚ Shudhangshu Shekhar Kundu Introduction: What an enzyme? Enzymes are protein molecules‚ which serve to accelerate the chemical reactions of living cells (often by several orders of magnitude). Without enzymes‚ most biochemical reactions would be too slow to even carry out life processes. Enzymes display
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BIOLOGY LAB REPORT (UNIT 7: ENZYMES) GENERAL Enzymes are protein that acts as catalyst‚ lowering the activation energy need for reactions to progress in cells. The reaction can still occur without the presence of the enzyme‚ but at a much slower rate. The activation energy is the minimum amount of energy need for a chemical reaction to occur‚ yielding from a given set of reactants. In enzymatic reactions‚ we have substrates which are reactants of reaction bound to an enzyme. While an active site is
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