THE EFFECT OF CHANGE IN pH ON THE ACTIVITY OF ENZYMES Introduction: Enzymes are catalysts that speed up‚ and control chemical reactions within a cell and break down complex molecules into smaller ones. Some of the chemical reactions that take place in the body produce toxic by-products which need to be converted. The liver produces hydrogen peroxide‚ which is extremely poisonous. When the catalase comes in contact with the hydrogen peroxide‚ it is broken down into water and oxygen. Aim:
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Biology coursework How does the temperature affect the rate of reaction? Introduction For our experiments‚ I was investigating what affects the rate of reaction. We used Hydrogen peroxide to test the rate of reaction‚ with the temperature of this being our variable that we changed. Hydrogen peroxide is a clear‚ colourless liquid which has various amounts of uses within the laboratory‚ industrial purposes and even in our households. It is mainly used for cleaning products and hair dye but is
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The Effect of pH on Enzyme Activity A piece of Solanum tuberosum (potato) was removed and mixed with distilled water in a blender. The resulting solution was filtered through multiple layers of cheese cloth to filter out the liquid by eliminating any large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of
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Enzymes are biologic polymers that catalyze the chemical reactions that make life as we know it possible. The presence and maintenance of a complete and balanced set of enzymes is essential for the breakdown of nutrients to supply energy and chemical building blocks; the assembly of those building blocks into proteins‚ DNA‚ membranes‚ cells‚ and tissues; and the harnessing of energy to power cell motility‚ neural function‚ and muscle contraction. With the exception of catalytic RNA molecules‚ or
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Experiment 10 – Enzymes Enzymes are proteins that act as catalysts for biological reactions. Enzymes‚ like all catalysts‚ speed up reactions without being used up themselves. They do this by lowering the activation energy of a reaction. All biochemical reactions are catalyzed by enzymes. Since enzymes are proteins‚ they can be denatured in a variety of ways‚ so they are most active under mild conditions. Most enzymes have optimum activity at a neutral pH and at body temperature. Enzymes are
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The Effect of pH on a Food Preservative September 18‚ 2012 Objective: To study the affect of pH on a food preservative. Chemical Equation: C6H5COONa + HCL ------ C6H5COOH + NaCl Procedure: This experiment was started with a clear solution of sodium benzoate and HCl was added to it‚ ultimately producing benzoic acid. First‚ .3395 g of sodium benzoate was weighed‚ then it was dissolved in water‚ causing it to disassociate into ions. Next‚ 3M of HCl were added drop wise to the solution
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Myrtle Freedom under threat: PH‚ 2rd deadliest country for Journalists Journalism has long been a high-risk profession‚ many journalists and reporters have put their lives on the line for a story. Journalists often have to go through extreme measures just to get information. Intensive research‚ live coverage and interviews are being demanded more and more by the public and in order to get such vital information‚ being on the scene is necessary. According to Committee to Protect Journalists (CPJ)
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Introduction Enzymes are proteins that are involved in all the chemical processes in living things. As they are made of proteins they are affected by pH and temperature. Enzymes are catalysts; they speed up chemical reactions without being changed themselves. Digestive enzymes speed up the breakdown of large food molecules into smaller ones so that the blood can absorb them. Enzymes turn a large starch molecule into thousands of tiny glucose molecules. Enzymes end in ’ase’. There are thousands of
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LAB 1: What temperature does the enzyme actually work properly in? (Hypothesis) If the temperature is below 40 but above 20‚ then the liver will show bubbles. If the temperature is raised higher than the optimum temperature‚ then an extreme decline in enzyme activity would occur following by the quick denaturing of the enzyme‚ rendering it is permanently useless. Also about 37°C is body temperature. The liver that was at 25°C had a huge amount of bubbles (a 4 on the scale) and the 0°C
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Enzymes are responsible for multiple reactions that take place naturally in the living organisms. The purpose of the enzymes lab was to investigate how the enzymes play a role in a reaction‚ affecting the rate of reaction (ROR). Interestingly‚ we tested how the enzymes affect the reaction rate at multiple temperatures (0‚ 23‚ 37‚ 50‚ 70‚ and 95 C). It was predicted that an increase in temperature will elevate the thermal activity of substrate which increases the chances the substrate molecules will
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