Enzyme Lab Name ___________________________ Assignment 1: Getting to Know Enzyme Lab: Setting Up an Experiment The first screen that appears in Enzyme Lab shows you a biochemistry lab containing all the reagents and equipment you will need to perform your experiments. Click on each item in the lab to learn more about its purpose. Once you are familiar with the lab‚ click on the Experiment button to begin the first assignment. This assignment is designed to help you become
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Effects of Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes‚ proteins that act as catalysts‚ are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes‚ the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol
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EXPERIMENT TO SHOW THE EFFECT OF TEMPERATURE ON ENZYME ACTIVITY Table of raw data of experiment to show effect of temperature on enzyme activity Temperature (ᵒc) (+/-0.05) | Time taken for pink solution to turn back to white (s) (+/- 0.05) | | Repeat1 | Repeat2 | Repeat3 | Repeat4 | Repeat5 | 21 (Room Temperature) | 414.0 | 286.0 | 286.0 | 297.0 | 294.0 | 40 | 122.0 | 88.0 | 125.0 | 86.0 | 92.0 | 60 | 176.0 | 162.0 | 161.0 | 166.0 | 180.0 | 80 | 356.0 | 367.0 | 396.0 | 85.0 | 438
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Experiment 5: Wittig Reaction Purpose The purpose of this experiment is to synthesize trans-9-(2-phenylethenyl) anthracene using a wittig reagent formed by reacting phosphonium chloride with base. Experimental In a reaction tube‚ (0.200g) benzyltriphenyl phosphonium chloride‚ (0.115g) anthraaldehyde‚ and (.6ml) dichloromethane (DCM) is mixed together with a stir bar. We stirred the mixture rapidly and added (0.26ml) of a 50% NaOH solution dropwise. The mixture was stirred for 20-30
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What is Enzyme? Enzymes are protein that acts as speed up reactions and break down molecules in our body. However‚ different enzymes only work on certain types of molecules. Enzymes can accelerate the reactions by more than one million times.(3) In our human body‚ there are a total about forty thousand types of enzymes and each catalyzes different kind of molecule.(3) The molecules that enzymes help to accelerate is called substrates‚ and when enzyme is combined together with the substrate‚ it
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certain solutions together and observing changes that occurred. PROCEDURE: 1 Cotton Swabs‚ 1 Sheet each of white and black paper‚ 1 Distilled water‚ Goggles-Safety‚ 1 Well-Plate-24‚1 Well-Plate-96‚ Bag-CK1 1 Pipet‚ Empty Short Stem‚ Experiment Bag Ionic Reactions 1 Barium Nitrate‚ 0.1 M - 2 mL in Pipet‚ 1 Cobalt (II) Nitrate‚ 0.1 M - 2 mL in Pipet1 Copper (II) Nitrate‚ 0.1 M - 2 mL in Pipet‚ 1 Iron (III) Nitrate‚ 0.1 M - 2.5 mL in Pipet‚ 1 Nickel (II) Nitrate‚ 0.1 M - 2 mL in Pipet‚ 1 Sodium Bicarbonate
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milk Introduction Enzymes are globular protein‚ responsible for most of the chemical activities of living organisms. They are made up of long chains of amino acids containing carbon‚ hydrogen‚ oxygen and nitrogen (Gunsch‚ 2012). The role of enzyme is to act as catalysts‚ substances that speed up chemical reactions without being chemically altered during the process. The speeding up of chemical reactions is done by lowering the activation energy required to start a reaction. Enzymes are specific in
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using a synthesized version of salivary amylase‚ this being Diastase. The starch will be placed into the Diastase and water and then placed in baths of water of different l. temperatures. The test tube containing water will have little or no reaction at all. However‚ the test tube containing the Diastase will have several different times to which the starch will break down. Once the Diastase reaches between 30 - 40 degrees Celsius‚ the time taken for the starch to break down should decrease
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Matthew Saldanha Bio DCP lab-Catalase experiment Aim: To investigate enzyme kinetics‚ using different concentration of the enzyme. Hypothesis: The assay system used in the lab consists of a filter paper disc coated with the enzyme and the dropped into a papercup of substrate (Hydrogen Peroxide). As the hydrogen breaks down the hydrogen peroxide into hydrogen and oxygen gas‚ the bubbles of oxygen gas collect underneath the filter and make it
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Interpretation and Evaluation of Results Interpretation: The results from the experiment determining the effects of pH on enzyme activity show that as the independent variable‚ pH‚ increases the dependent variable‚ percentage transmission‚ decreases. This is shown in the results as at the lowest pH‚ pH 4.0 the average percentage transmission is at its highest at 97%. At the highest pH‚ pH 8.0 the average transmission is 78.5%. This is also supported from the graph as it produces a negative gradient
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