Chirag Patel Biology 157 Determining the Effect of Varying Substrate Concentration Question: How does concentration of potatoes affect the amount of oxygen produced with hydrogen peroxide and potatoes (enzymes)? Introduction The enzyme used for this experiment is Catalase. Catalase is inside mostly any living organism which uses oxygen. Its job is to break down hydrogen peroxide‚ into oxygen and water. (Formula) 2H2O2 ---> 2H2O + O2 (lab manual). There are limiting factors which if altered
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Enzymes What Are Enzymes? Substances that speed up chemical reactions are called catalysts. Organic catalysts are called enzymes. Enzymes are specific for one particular reaction or group of related reactions. Many reactions cannot occur without the correct enzyme present. They are often named by adding "ase" to the name of the substrate. Example: Dehydrogenases are enzymes that remove hydrogen. Induced-fit Theory The shape of the enzyme must match the shape of the substrate
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Lambda DNA with restriction enzymes. Aim: The objectives of this experiment are: Become more familiar with using micropipettes. Use restriction enzymes to cut DNA at specific sites. Use Ligase to rejoin some of the cut/separated DNA fragments. Learn to separate DNA using electrophoresis. Introduction: Restriction enzymes are proteins which cut dsDNA at specific regions depending on the enzyme used‚ determined by the nucleotide sequence of the DNA‚ i.e. each enzyme recognises specific nucleic
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separation of volatile substances mixed with nonvolatile substances by boiling the mixture to alter the phase of the volatile substance. The researchers used the simple distillation set up to conduct the experiment. 25 ml of The Bar Vodka was used and 7 ml of distillate was collected before a temperature of 95 degrees Celsius was reached. Collected distillate was then subjected to a flammability test to confirm ethanol content. The percent ethanol was computed to be 24%. Introduction Distillation
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Enzymes An enzyme is a protein used to speed up the rate of a chemical reaction. Because they regulate the rate of chemical reactions‚ they are also called catalysts. There are many‚ many different types of enzymes‚ because for each chemical reaction that occurs‚ an enzyme specific to that reaction must be made. To act on a substrate‚ an enzyme must contain an active site. The active site is the area on the enzyme that allows the substrate and enzyme to fit together. The amino acids that are present
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Enzyme action is the simple mechanism by which enzymes catalyse chemical reactions. This begins with the binding of the substrate to the active site on the enzyme. The binding of the substrate to the enzyme causes changes in the distribution of electrons in the chemical bonds of the substrate. This then causes the reactions that lead to the formation of products that are then released from the enzyme surface to regenerate the enzyme for another reaction cycle. The active site has a unique shape that
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------------------------------------------------- Enzyme Pre-Lab Harry Kang 9/26/12 1) The purpose of this lab was to determine the rate of enzyme activity under variety of different conditions‚ such as‚ different amount of drops of enzymes and different temperature of water. The class measured the pressure in the test tube during the reaction of the substance with‚ 1.5 ml of H2O2‚ 1.5ml of H2O and different amounts of enzyme drops‚ to determine how much oxygen gas is produced during the reaction since the pressure of
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Effecting the Reaction Rate in Enzymes in Plants and Animals Problem- How do variables temperature and surface area affect the rate of reaction occurring in the enzyme? Information- Enzymes are proteins that act as biological catalysts. They speed up chemical reactions that take place in cells. Enzymes can be found in both liver and potato. They provide a site where reactants can be brought together to react. These reactants are called substrates that fin into the enzyme. The changing of
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Enzymes in Living Tissues Purpose: The purpose of the lab was to analyze the enzymes in living tissues‚ represented by the pieces of liver. Also‚ hydrogen peroxide was used to demonstrate these effects. Storyboard: Materials: 2 50 mL beakers 10 mL graduated cylinder 3% hydrogen peroxide solution Hot water bath Lemon juice or HCl Fresh liver Forceps Procedure: Measure 10 mL of hydrogen peroxide and record its temperature. Pick the liver up with the forceps and
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Nicole Buckman BIO220 March 18‚ 2013 Meredith Morrissey Controlled Burns and the Environment In a forests life‚ the process of a fire starting and destroying the trees and underbrush in a forest is an important cycle. It helps to improve the life span of the vegetation and animals living in it. With the rise in wildfires all over the country‚ researchers say that controlled burns will help reduce the risk of catastrophic wildfires. Many people think of fire as only being dangerous and destructive
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