EXPERIMENT 2: COMMON-ION EFFECT AND BUFFERS MARVILE REA R. FERRER1 1DEPARTMENT OF MINING‚ METALLURGICAL & MATERIALS ENGINEERING‚ COLLEGE OF ENGINEERING UNIVERSITY OF THE PHILIPPINES‚ DILIMAN‚ QUEZON CITY 1101‚ PHILIPPINES DATE SUBMITTED: DECEMBER 13‚ 2012 DATE PERFORMED: DECEMBER 07‚ 2012 ______________________________________________________________________________________________________________ INTRODUCTION Monitoring the pH range of a laboratory reaction or a process is very
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Catalase Lab Introduction: Catalase is an enzyme normally found in many plant and animal tissues. Its purpose is to destroy toxic substances which may be introduced into cells. Also‚ some cells use catalase to destroy cellular debris or worn out organelles. In this lab‚ we will use a catalase solution from potatoes and determine the effect of temperature and pH on the action of this enzyme. The substrate of the enzyme will be 3% hydrogen peroxide (H2O2). Catalase works by the following mechanism:
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LABORATORY REPORT Activity: Enzyme Activity Name: Daniel Franco Instructor: Professor Jennifer Frere Date: 03.08.2015 Predictions Sucrase will have the greatest activity at pH 6 Sucrase will have the greatest activity at 60 °C (140 °F) Sucrase activity decreases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature‚ amount of substrate
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| Case Study/Reaction Paper | The Stanford Prison Experiment | | Reviewing and Analyzing: The Stanford Prison Experiment The Stanford Prison Experiment was conducted at Stanford University in California from August 14 to August 20‚ 1971. Led by psychology professor Philip Zimbardo‚ twenty-four male students whom were chosen out of 75‚ were randomly assigned roles of prisoners and guards‚ for an investigation into the causes of conflict between military guards and prisoners. According
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Controlled Burns Controlled burning is helpful and can be used for many reasons that are beneficial to the forest or burnt area. Controlled burns can; prevent further naturally started fires by burning out all the leftover dry material‚ get rid of dead plants to make room for new vegetation to grow which in turn creates new homes for animals‚ and can even help the soil gain nutrients. Some believe that controlled burning isn’t necessary and that the chance for the fire to go out of control is too
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Cellular Biology furthers technology-Enzymes in the Dairy Industry Since ancient times‚ enzymes have played an important role in food production. Especially in the diary industry‚ some enzymes are required for the production of cheese‚ yogurt‚ and other dairy products‚ while others are used in a more specialized fashion such as improving texture or flavour of the product. Enzymes are used to catalyze the desirable reactions in industrial processes. Today‚ enzyme applications in such processing get
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Potato Enzyme Lab INTRODUCTION An enzyme is a protein that speeds up or slows down a specific chemical reaction in an organism. A good rule of thumb is to remember that enzyme names end in “-ase”. This will help in identifying enzymes in further readings. Generally enzymes are catalysts. Hydrogen peroxide is a toxic chemical that is produced in many organisms during metabolism. Organisms must get rid of this toxin to survive. One reaction turns the hydrogen peroxide into water and oxygen. The
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Discovery Restriction enzymes were discovered 40 years ago during investigations into the phenomenon of host-specific restriction and modification of bacterial viruses. Restriction enzymes protect bacteria from infections by viruses‚ and it is generally accepted that this is their role in nature. They function as microbial immune systems. When a strain of E. coli lacking a restriction enzyme is infected with a virus‚ most virus particles can initiate a successful infection. When the same strain
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In this lab we tested the effect of temperature has on the rate of enzyme activity. The way we figured this out was by taking four different temperatures and testing the difference absorbance levels they produced every 20 seconds for about 2 minutes straight using a spectrophotometer. The important part of this experiment was the temperature the enzyme concentration was made at. What we got from the experiment was at lower temperature we got very low numbers for the absorbance‚ which gave us a lower
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panel above to save your report) Activity: Enzyme Activity Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 60 °C (140 °F) 3. Sucrase activity decreases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables. temperature; amount of substrate (sucrose) present; sucrase
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