Abstract The aim of this experiment was to investigate the effect of temperature on the enzyme catalase. The original research question was exploring the effect temperature would have on a yeast catalase reacting with hydrogen peroxide (H2O2). To address the latter question a series of experiments were conducted. The various temperatures experimented with were as follows: 22 degrees Celsius (room temperature)‚ 0 degrees Celsius (freezing)‚ 100 degrees Celsius (boiling)‚ and 37 degrees Celsius.
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Critical Appraisal of a Quasi-Experimental Design Nursing research is an essential part of a nurse’s profession; it has a remarkable influence on current and future evidence-based practice (Tingen‚ Burnett‚ & Murchison‚ 2009). Therefore‚ it is vital that nurses develop the skill of critiquing a research paper to know how to read and understand those reports. Because evidence-based practice is embedded in the foundation of nursing‚ it is vital that nurses develop the ability to recognize research
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Contents 1 Design 2 1.1 Defining the problem 2 Research Question 2 Hypothesis 2 Background Information 2 Investigation Variables 3 1.2 Controlling Variables 4 Control Variables 4 Uncontrolled Variables 4 Control Experiment 4 1.3 Experimental Method 5 Materials and Equipment 5 Method 5 Practical Safety and Risk Assessment 6 Setup Diagram 6 2 Data collection and processing 7 2.1 Recording Raw Data 7 Sufficiency of Data 7 Results – Raw Data 7 Qualitative Observations:
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We are studying the effect of pH on catalase activity. Catalase by scavenging hydrogen peroxide to water and oxygen is an important enzyme of cell defense mechanisms against oxidative stress in all living organisms (Dat et al.‚ 2003). Cells use catalase because it is the most efficient enzyme as an antioxidative enzyme which lowers hydrogen peroxide or superoxide to accumulate to toxic levels in plant growth (Bowler et al. 1992). The formula that involves catalase as the enzyme is . pH is a measure
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General Biology I Spring Semester 2013 Lab Report Name: Date: 03/20/2013 Lab: # Purpose of this lab: To test for the effects of temperature on catalase activity. Introduction: Enzymes are proteins that act as catalysts and help complex reactions to occur everywhere in life. Enzymes affect a chemical reaction by acting as a catalyst‚ which is to say‚ it speeds up the reaction. However‚ extreme ph and temperature hinders enzyme function. Optimal activity of an enzyme
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Setting This randomized experimental study was completed in a clinical setting. Treatment rooms provided a comfortable environment‚ free from clutter and visual and auditory distractions. Each treatment room contained furniture and equipment necessary to complete activities of daily living including a bed with a fitted sheet‚ chair‚ table‚ lamp‚ and a cabinet. Other small objects needed to complete tasks (pill box‚ cup‚ pillow‚ pen‚ paper‚ blanket) were organized to minimize both clutter and
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Systematic Error Systematic error is a short phrase that is usually easy to find in the science classes. Usually‚ there are lots of different ways to define this phrase. So‚ what is the exact meaning of systematic error? Systematic error is one of the biases in measurement which could reduce the accuracy of the result of the measurement and cannot attribute to chance. Systematic error is a kind of bias in measurement. Literally‚ it leads to the situation where the mean of many separate measurements
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The Effect of Temperature on the Enzyme Catalase Stephen Francis Biology 183 Abstract This experiment was performed to determine the resultant effect of temperature change on the reaction between the enzyme catalase and hydrogen peroxide. This experiment was performed by measuring and comparing the amount of oxygen bubbles produced and the absorbance of the catalase and hydrogen peroxide solution over time at room temperature‚ 2°C‚ 50°C‚ and 60°C. The overall result of this experiment proved
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Worksheet — Summary of identified misstatements Entity Period ended 335 Page 1 of 2 Objective: To document misstatements identified during the audit and to evaluate: - The effect of identified misstatements on the audit. - The effect of uncorrected misstatements‚ if any‚ on the financial statements. Performance materiality Insignificant misstatements under $ need not be recorded below. Amount of over (under) misstatement in the financial statements Circumstances of occurrence
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Basic Concepts of Error Analysis 1. Significant Figures: The laboratory usually involves measurements of several physical quantities such as length‚ mass‚ time‚ voltage and current. The values of these quantities should be presented in terms of Significant Figures as follows. For example‚ the location of the arrow is to be determined in Fig. 1. It is obvious that the location is between 1 cm and 2 cm. The correct way to express this location is to make one more estimate based on your intuition
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