independent variable in this investigation is the varying concentrations of the substrate (Hydrogen Peroxide: 1%‚ 3%‚ 5% and 6%)The dependent variable was the rate of enzyme catalase activity‚ which was measured by the volume of froth produced after one minute.The concentration and volume of liver extract (source of catalase) used was kept constant at 10ml per solution. This factor was kept constant‚ as the concentration of the enzyme affects the rate of reaction; higher enzyme concentrations increase
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launch a reaction or speed it up. Catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). The catalase used in this experiment will come from five different sources: Spinacia oleracea (Spinach)‚ Brassica oleracea (Broccoli)‚ Solanum tuberosum (Russet Potato)‚ Malus domestica (Apple)‚ and Allium cepa (Onion). The five different catalases from the sources will all be used
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[Name] [Teacher] [Course] [Date] Catalase Lab Report An enzyme is something that helps to speed up a chemical reaction. The enzyme changes from reaction to reaction‚ but it always has the same impact. However‚ certain variables may cause the enzyme to have a more or less significant impact on the speed of each reaction. One of these variables that changes the effectiveness of an enzyme is temperature. There is an optimal functioning temperature for each enzyme in each reaction‚ depending on
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particular lab‚ temperature was the environmental factor studied. More specifically‚ the enzyme catalase and its substrate hydrogen peroxide were tested under different temperatures. It was discovered that‚ temperature can affect the optimum operation of enzymes; The enzyme catalase has an optimum operation condition (in this case it would be a specific temperature) in which the enzyme’s efficiency is at its highest. To achieve the data‚ six different pieces of beef liver (the source of catalase) were
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EFFECT OF TEMPERATURE ON THE RATE OF CATALASE REACTION IN HYDROGEN PEROXIDE INTRODUCTION Enzymes function similarly to a lock a key mechanism where only one specific key will work (U and Fischer‚ 1994). An enzyme is a macromolecule that acts to increase the rate of either a substrate breaking down into two or more products‚ or where two substrates join up to form one product (Dundon‚ 2018). These enzymes perform this by lowering the activation energy required for the reaction to occur under normal
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measured and recorded by the class. These measurements were to determine error in measurements and the derived quantities‚ volume and density. The diameter was determined using a dial caliper and the mass‚ a triple beam balance. The results of the error for diameter were not as expected while the results of the volume and density were deviated greatly from expectations; only the weight had a small error. Experimental Instruments: 1. Steel ball 2. Dial caliper (Mano Stat Corp.) 3. Triple
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Introduction: Catalase is an enzyme‚ which is present in all life forms that utilize oxygen for their biological processes. Enzymes are proteins which increase the rate of reaction by decreasing the activation energy (the energy required to initiate a reaction). One of the main function of Catalase is to prevent the accumulation of hydrogen peroxide (H2O2) in the body by breaking it down into water and oxygen gas. Hydrogen peroxide is a toxic by-product of metabolic reactions. If hydrogen peroxide
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Research Question: How will an increase of FeSO4 concentration affect the activity of the enzyme catalase? Hypothesis: By increasing the concentration of FeSO4‚ there will be a decrease in the activity of the enzyme catalase because the Iron (ii) in the metallic ion will act as an inhibitor during the catalysis of the hydrogen peroxide into O2 and H2O. Introduction: We rely on oxygen to provide us with energy (ATP) and go through all the phases of aerobic respiration. However‚ oxygen can be
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In this experiment we are trying to determine the effect temperature has on catalase activity. We started out with five test tubes and then labeled them as we went about‚ after we added a catalase extract to each tube. Once added to each test tube they all sat in their respected temperature for ten minutes‚ so they could have a full effect. Once the time was up each was then collected and then we began to shake them from side to side for the foaming to start. When the foam settled‚ we discovered
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the bacterium was a gram negative or a gram positive. After performing the gram stain‚ I concluded that by the appearance of purple spherical clusters resembling grapes that is was a gram positive cocci. A Catalase test was then performed using hydrogen peroxide. A positive catalase test was observed when bubbles started to form on the culture distinguishing Staphylococcus spp. form on the culture distinguishing Staphylococcus spp. from Streptococcus spp. Finally‚ a Mannitol Salt Agar
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