Experiment 2: IDENTIFICATION OF UNKNOWNS BY TLC AND MP IN COMBINATION Introduction: Thin layer chromatography (TLC) is one of the most valuable techniques in organic chemistry. This is a best method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that is stationary and one that is moving or mobile. Chromatography works on the principle that different compounds will have different
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Design Experiment: Enzyme Inhibitors. Research question: What is the effect of adding lead nitrate solution on the activity of amylase enzyme? Aim: To test the effect of adding nitrate solution on the activity of amylase. Background Information: Inhibitors are molecules which repress or prevent another molecule from engaging in a reaction. They are substances that attach themselves onto an enzyme and reduce or prevent the enzyme’s ability to catalyse reactions. Competitive Inhibitors are inhibitors
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Light Absorption by Photosynthetic Pigments in Silver Beet Abstract The aim of the experiment was to determine the absorption of differing wavelengths of light by individual pigments in the vegetable silver beet. Pigments were extracted from silver beet leaves and separated into chlorophyll a‚ chlorophyll b‚ and carotene via chromatographs. Chlorophylls were then separated and an absorption spectra created using results obtained from a spectrophotometer. It was clear there were two distinct peaks
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Photosynthesis and Cellular Respiration Nina R. McCray May 5‚ 2015 Biology Lab (SCIE207-1502-A-07) LaDonna McCowan-Ferrier Photosynthesis and Cellular Respiration Lab Report Introduction This is an investigation of the effect of the intensity of light (photosynthesis) have on the rate of cellular respiration (measured as the number of oxygen bubbles). We will determine whether or not it is possible to examine the relationship between photosynthesis and cellular respiration under controlled
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Chapter 6-Intro to Metabolism METABOLISM= all the chemical reactions in an organism CATABOLIC PATHWAY (CATABOLISM)• release of energy by the breakdown of complex molecules to simpler compounds EX: digestive enzymes break down food ANABOLIC PATHWAY (ANABOLISM) • consumes energy to build complicated molecules from simpler ones EX: linking amino acids to form proteins ORGANISMS TRANSFORM ENERGY ENERGY- capacity to do work KINETIC ENERGY- energy of moving objects POTENTIAL ENERGY- energy
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The effect of water and fertilizer on corn seedlings Biology 1108 Lab Section November 7‚ 2012 Abstract The effect of water and fertilizer on corn seedlings was examined. It was hypothesized before the experiment that enough water with sunlight and air should make the plants grow healthy. It has to do with photosynthesis. Fertilizer is also going to make the plants grow much faster; this is because of the nutrients in it. The experiment took about four to six weeks to complete; it took some
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Bugs Attraction to Colored Lights Introduction: This experiment was conducted to see which colored light pill bugs would prefer more out of red‚ blue‚ yellow‚ and green lights. If pill bugs went to the green and yellow lights more than the blue and red lights‚ then this means they like brighter lights more than gloomier lights. For the experimental group‚ the colors used were blue‚ red‚ yellow‚ and green. The controlled group was the middle chamber. It got regular light from the classroom. The independent
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Evaluation Like any experiment‚ there were a number of potential errors during the procedure of the experiment. Errors could have arisen as a result of the uncertainties associated with the instruments I used to take measurements‚ and also as a result of errors associated with the actual method. Of course‚ due to the limitations of the procedure‚ they could not be eliminated completely‚ so I will explain what I did to reduce them to an acceptable level and how I could have improved my method to
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of the investigation was to investigate the effect of substrate concentration Hydrogen Peroxide H O (in %) on the rate of reaction of the enzyme catalase (in 1/mean time). Prediction: As the substrate concentration (hydrogen peroxide) in % increases the rate of reaction in 1/mean rate increases until the solution becomes saturated with the substrate hydrogen peroxide. When this saturation point is reached‚ then adding extra substrate will make no difference. The rate steadily increases when more
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Rf values using the collected data‚ and study photosynthesis with isolated chloroplasts. Light energy Light energy Background Information (Activity A): In photosynthesis‚ plant cells convert light energy into chemical energy that is stored in sugars and other organic compounds. It is an endergonic and anaerobic reaction. Critical to the process is chlorophyll‚ the primary photosynthetic pigment in chloroplasts. The chemical equation for photosynthesis is: 36 ATP + 6CO2 + 6H2O C6H12O6
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