62 Iodine test for starch Amount of starch remaining Enzyme activity level Dark blue-black All None (0) Blue Most Low (1) Light brown Some Moderate (2) Gold None High (3) Part 1: Effect of Enzyme Concentration 1. Label five test tubes 1-5. Place 4 mL of 1 % starch in each of the first four test tubes. Place 4 mL of amylase solution in the fifth tube. Place all of the tubes in the 37°C water bath for 5 minutes. Obtain 5 clean droppers and label them 1-5. (To avoid contamination of these solutions
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Enzymes: Food & Nutrition What are enzymes Enzymes are a type of protein produced by a living organism used to catalyze chemical reactions in cells. These reactions allow the cell to build things or take things apart as needed in order to grow and reproduce. How do enzymes work - in steps 1) Substrate floats near enzyme 2) Substrate and enzyme connect – which breaks it into products 3) Products are released ex) BreadFast & Co.’s use of enzymes The company uses many different
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Enzymes are globular proteins that are synthesized by the ribosomes in a cell. They act as catalysts during biological reactions; therefore‚ enzymes are able to speed up these reactions without undergoing a permanent change themselves. These proteins are able to do this by lowering the activation energy of a reaction. To add on‚ enzymes require specific conditions under which they can work best. Reactions occur at faster rates when the temperature is higher. However‚ the rate of an enzyme-catalyzed
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Factors Affecting Enzyme Activity Abstract: In the following lab factors affecting enzyme activity‚ temperature‚ pH‚ enzyme concentration‚ substrate concentration and surface area will be tested on a beef liver enzyme to see if there will be any effect of performance. By doing 2 or more trials the results will show whether there is an effect to the enzyme from the following factors or not. Some of the factors may denature the enzyme and some will do nothing. Using a table qualitative and the
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Factors Affecting The rate of Enzyme Activity Prediction: As the temperature increases the rate of enzyme activity will also increase‚ thus increasing the rate of reaction. However‚ if the temperature is too high the enzyme will denature. Materials: 4 test tubes 2 small beakers A dozen filter paper disks Test tube rack Hydrogen peroxide (H2O2) Potato extract Forceps Thermometer Hot plate Large beaker Ice cubes Graduated cylinder Stopwatch Procedure: Step 1 Place 10 mL of potato extract
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Chemistry 512 Enzyme Catalysis Lab Report Pre-lab Questions: 1. Write a balanced chemical equation with state symbols for the reaction catalyzed by peroxidase. 2H2O2 2H2O + O2 (4H1 4O) (4H + 2O + 2O) 2. What is the substrate(s) of this reaction? What is the catalyst? Substrate = H2O2 hydrogen peroxide Catalyst = peroxide 3. At what approximate temperature do enzymes normally operate in the body of a warm-blooded animal? Would your answer change if the enzyme came from a plant or yeast? Enzymes normally
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Biology Enzyme Catalase Investigation Aim: The aim of this investigation is to study and observe whether or not the concentration of hydrogen peroxide (varying from 10 – 30 millilitres) affects the rate of reaction. Hypothesis: With the increase of the concentration of Hydrogen Peroxide 3% substrate‚ I prediction that the rate of pressure increase will begin to amplify. The pressure is bound to increase because the catalase quickly reacts with the hydrogen peroxide; this is why the more substrate
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Enzyme Lab Introduction/ Abstract An enzyme is a substance produced by a living organism that acts as a catalyst to bring about a specific biochemical reaction. They are mainly made up of proteins and can tremendously speed up reactions. E. coli ( a bacterium) has about 1‚000 different types of enzymes floating around in its cytoplasm at any given time. Enzymes can be used to join and even break up molecules as shown in the diagram below. (1)
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Enzymes lower the amount of energy needed in a chemical reaction. This happens because enzymes are catalysts‚ so they speed up the activation rates that occur in living organisms. Without enzymes‚ it would be difficult to break down particles like food in the digestion system. Enzymes are all very specific to what chemical reactions they will work with‚ and the temperature‚ pH‚ and salt concentration have to be a specific levels in order for the enzyme to function. The structure of each enzyme has
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Anatomy of Flowering Plants Tissues A tissue is a group of cells having a common origin and usually performing a common function. Based on cell’s capability to divide‚ tissues are classified into two main groups which are as follows: 1. Meristematic and 2. Permanent tissues. Meristematic Tissues: Cells in the meristematic tissue are capable of dividing. Meristematic tissues are found in those regions which need to grow continuously. For example‚ root tips and stem tips contain meristematic
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