Enzyme Lab Using Jello INTRODUCTION: Enzymes are known as protein catalysts. The name protein catalyst suggests that most enzymes are made of proteins. A catalyst is a substance that speeds up chemical reactions without being consumed in the process. (Giuseppe‚ M 2002‚ p.69). After a reaction has been catalyzed‚ the catalyst can be used again to catalyze the same reaction. Enzymes reduce the activation energy (minimal energy) it takes for a reaction to take place. Enzymes can either catabolize
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Six Factors Affecting the Performance of an Enzyme Enzymes are considered catalysts; substances that increase the rates of reactions. Enzymes are responsible for thousands of metabolic processes that involve the sustainment of life‚ one common one is simple food digestion. Without enzymes‚ digestion would occur too slowly for life to continue. Enzymes maintain a protein structure consisting of one or possibly more than one polypeptide chains of defined primary structure‚ and take up a characteristic
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DIGESTIVE ENZYMES AND THEIR FUNCTIONS Mouth -the oral cavity or the mouth contains salivary glands that secrete a wide range of enzymes to aid the first step of metabolism of food. The salivary gland secretes the enzyme lysozyme which has an antibacterial action. The enzymes secreted by the mouth mainly provide protection against bacteria. Betaine helps to maintain a fluid balance inside the mouth. The lists of digestive enzymes secreted by the oral cavity are mentioned in the table. Enzymes | Function
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Effect of different temperatures on the rate of an enzyme controlled reaction I will place starch and amylase into five water baths which are at different temperatures‚ and record the time it takes to break down the starch in the solution. Independent variables The independent variable is what I am going to change in my experiment. In this case it is the temperature of the water in the five water baths- 10‚ 25‚ 40‚ 55‚ 70 degrees Celsius Dependant variable This is what will stay the
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Abstract The main goal of the enzyme kinetics experiment was to see how the phosphatase-catalyzed hydrolysis of p-nitrophenyl produced p-nitrophenol in the presence of phosphate and fluoride ion inhibitors of various concentrations. The calculated Km constant was found to be 0.22 for all reactions. The Vmax values for each inhibition ion were 0.00986 for the phosphate ion and 0.00436 for the fluoride ion. The inhibitor constant‚ Ki‚ was determined to be 0.0967 for the phosphate ion. The inhibitor
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------------------------------------------------- Enzyme Pre-Lab Harry Kang 9/26/12 1) The purpose of this lab was to determine the rate of enzyme activity under variety of different conditions‚ such as‚ different amount of drops of enzymes and different temperature of water. The class measured the pressure in the test tube during the reaction of the substance with‚ 1.5 ml of H2O2‚ 1.5ml of H2O and different amounts of enzyme drops‚ to determine how much oxygen gas is produced during the reaction since the pressure of
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Explain the use of enzymes in food production by means of examples. You must include the example of lactase. Enzymes are proteins that speed up the rate of chemical reactions (up to a million times) in living organisms. Acting as catalysts they are not consumed nor altered in the process of converting the specific set of reactants into specific products. In food production‚ enzymes are greatly appreciated by their accelerated effect in biochemical processes and are mostly used in what we
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Experiment 4 – Effect of Temperature on Enzyme Activity Aim To study the effects of temperature on the activity of amylase enzyme on starch solution. Introduction Enzymes are widely known as biological catalyst. Almost all cellular reactions are controlled and guarded by enzymes. Virtually every metabolic reaction which takes place within a living organisms are catalyzed by enzymes. Enzymes are complex three-dimensional globular proteins. Some of the enzymes are built up off proteins and some
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Enzymes and their importance in plants and animals (25 marks) Enzymes are biological catalysts‚ which accelerate the speed of chemical reactions in the body without being used up or changed in the process. Animals and plants contain enzymes which help break down fats‚ carbohydrates and proteins into smaller molecules the cells can use to get energy and carry out the processes that allow the plant or animal to survive. Without enzymes‚ most physiological processes would not take place. Hundreds of
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Environmental Parameters of Enzyme Activity Alex Rocha Texas State University Abstract If you’ve ever left a cut up apple out for long‚ you’ll notice that after a while‚ it will turn brown. The reason for this is an enzyme named catechol oxidase‚ a ubiquitous plant enzymes containing a dinuclear copper center (Klabunde‚ Eicken‚ Sacchettini‚ & Krebs‚ 1998). In this experiment‚ we used two different chelators‚ ethylene diamine tetraacetic acid and phenylthiourea to test which would stop
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