The acid-fast stain is performed on samples to demonstrate the characteristic of acid fastness in certain bacteria. Acid fastness is a characteristic that is shared by just a few organisms‚ so staining to determine if organisms possess this trait is useful in microbial identification schemes. The Ziehl-Neelsen method has endured as a reliable and effective way to demonstrate the acid-fast bacteria. Materials: 18-20 nutrient hour agar slant culture of Staphyloccus aureus 4 day old nutrient
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solution of an acidic and organic compound and purify its respective acidic product through the techniques of an acid base extraction‚ recrystallization‚ and use of Melt Temp (melting point range). The reaction of an unknown acid (3-chlorobenzoic acid) with diethyl and sodium hydroxide was observed‚ and the solution naturally separated into an aqueous and organic solution. The mixture of acid and organic solution was created and then separated through the Hirsch funnel into separate beakers of an aqueous
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Part A: Table 1: Fractionation of amino acid Fraction No | Response to Ninhydrin test(level of blue colour change) | Response to Sakaguchi test (colour change) | 1 | X | yellow | 2 | Dark blue | yellow | 3 | Dark blue | Pale orange | 4 | Blue | Pale orange | 5 | Pale blue | Pale orange | 6 | Blue | Pale orange | 7 | Pale blue | Pale orange | Discussion Part A In the separation and purification of a single protein or amino acids‚ a solution containing the desired analyte
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Product Life Cycle Check A Guide© Translation Henrik Wenzel and Nina Caspersen‚ Institute for Product Development‚ Anders Schmidt‚ dk-TEKNIK Special edition adapted for course 42372‚ Tech.University of Denmark by dr. Michael Hauschild‚ September 2000. © IPU. First draft can only be used outside IPU with special permission. -do not copy- Preface This Guide has been written in connection with the project with the Danish title Stimulering af mindre virksomheders interesse for arbejdet med
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Acid-Base Calculations The Ion-Product Constant for Water‚ Kw Water undergoes ionization to a small extent: H20(l) H+(aq) + OH–(aq) The equilibrium constant for the reaction is the ion-product constant for water Kw: (1) This is a key equation in acid-base chemistry. Note that the product of [H+] and [OH–] is a constant at a given temperature (Eq(1) value is for 25oC). Thus as the hydrogen ion concentration of a solution increases‚ the hydroxide ion concentration decreases
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Separation of a Carboxylic Acid from a Neutral Compound by Extraction Reference: Smith‚ Chapter 2 (Acids and Bases) Introduction Carboxylic acids and phenols are two families of organic compounds that contain carbon‚ hydrogen and oxygen‚ and also react with water to yield an excess of hydronium ions over hydroxide ions. Pure water has a pH of 7‚ which means it has a hydronium ion concentration‚ [H3O+] of 10-7 M (M = molarity‚ moles/Liter). The hydronium ions in pure water come from the self-ionization
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Acid and Base Titrations: Preparing Standardized Solutions Introduction: This experiment focuses on titrations of acids and bases. A titration depends on addition of a known volume of solution and is a type of volumetric analysis. Many titrations involve either acid-base reactions or oxidation-reduction reactions. In this experiment we do one of each. We monitor the pH of the reaction with the use of a color indicator. We also learn about the standardization of bases (NaOH) and acids (HCl) which
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Macrocycle shuttling process occurs either through electrochemical oxidation of the benzidine residue or protonation of the basic nitrogens on this station. Both of these methods rely on the repulsive electrostatic interactions between the positively charged axial component and the post-switching cationic nature of the benzidine station (Figure 5.4). Deuterated trifluoroacetic acid (d-TFA) was added in the solution which resulted in the protonation of the
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During the year of 1675‚ three main events happen: King Philip’s War (1675-1676)‚ Bacon’s Rebellion (1675-1676)‚ and Board of Trade. These three main events started the development of English North America. These events alter the course of American history with making amendments that to this day some still ignore. The first event that happen as King Philip’s war; a war between New England and Native Americans. The Native Americans were starting to depend on English goods such as food‚ equipment
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Alanine (ala‚ A) (neutral‚ non-polar) Arginine (arg‚ R) (basic‚ polar) Asparagine (asn‚ N) (neutral‚ polar) Aspartic Acid (asp‚ D) (acidic‚ polar) Cysteine (cys‚ C) (neutral‚ slightly polar) Glutamic Acid (glu‚ E) (acidic‚ polar) Glutamine (gln‚ Q) (neutral‚ polar) Glycine (gly‚ G) (neutral‚ non-polar) Histidine (his‚ H) (basic‚ polar) Isoleucine (ile‚ I) (neutral‚ non-polar) Leucine (leu‚ L) (neutral‚ non-polar) Lysine (lys‚ K) (basic‚ polar) Methionine (met‚
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