Genomic Library A genomic library is a collection of recombinant vectors that represent the entire genome of an organism. Below is a brief outline of how to create the genomic library: 1. We isolated Vibrio fischeri DNA to pGEM genomic DNA using a phenol chloroform purification method. 2. Vibrio fischeri DNA and pGEM DNA was cut with restriction enzyme Sal I to prepare both for ligation. 3. Vibrio fischeri and pGEM DNA were ligated with T4 ligase. 4. E.coli DH5α competent cells were prepared
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Independence – Bipin Chandra Facets of Indian Culture - Spectrum Pub. Indian Culture Chapter of Indian Year Book Journals/Websites: The Hindu‚ www.pib.nic.in‚ Yojana‚ The Indian Express‚ Economic Political Weekly‚ Frontline 2. Modern Indian history from about the middle of the eighteenth century until the present significant events‚ personalities‚ issues. References: Indian Freedom Struggle of Independence – Bipin Chandra “A Brief History of Modern India” – Spectrum Pub. Indian Culture
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Romeo and Juliet play Act 2 Scene 2 Romeo and Juliet are clearly complex characters; they go through an intense journey. They learn about themselves as individuals‚ unit and in relation to the people around them. Their relationship is based on passionate attraction that transforms into something powerfully emotional‚ with real depth. Their relationship shows us the juxtaposition of the clear love and hate woven throughout the play. They fight to keep their relationship together‚ the reader is touched
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Chapter 2 Blades‚ Inc. Case Ben Holt‚ chief financial officer (CFO) of Blades‚ Inc.‚ has decided to counteract the decreasing demand for Speedos roller blades by exporting this product to Thailand. Furthermore‚ due to the low cost of rubber and plastic in Southeast Asia‚ Holt has decided to import some of the components needed to manufacture Speedos from Thailand. Holt feels that importing rubber and plastic components from Thailand will provide Blades with a cost advantage ( the components
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expansion of an existing business‚ by an individual‚ a team of individuals‚ or an established business‚” suggest the following: • • • • • • Promoting entrepreneurship‚ especially outside the most active age group (25– 44)‚ with specific programs that support entrepreneurial activity. Facilitating the availability of resources to women to participate in the entrepreneurial process. Committing to long-term‚ substantial postsecondary education‚ including training programs designed to develop skills required
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philosopher‚ and a teacher‚ who lived from 469 to 399 BC. He is known up until this time because of the contributions he had made in this world. He is famous for his brilliant ideas about the philosophy of man. One of Socrates’ basic principles about man and life is to “know thyself.” In this theory‚ Socrates explains that man must first know who he really is‚ his talents‚ abilities‚ passions‚ desires‚ and he must live according to it. If you are good with something‚ you must pursue it and you shall
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Leilani Melenciano November 18‚ 2014 One of the most famous Greek philosophers was named Socrates. His students‚ named‚ Plato and Xenophon revealed how his way of thought exerted a philosophical influence on ancient and modern philosophy. One of Plato’s works‚ Apology‚ depicts Socrates insight‚ integrity‚ and argumentative skill. Just as Socrates had believers‚ he also had non-believers. One of his foes went by the name of Aristophanes. Aristophanes’ Clouds‚ is a comedy that was intended to make
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Manguni‚ Jasper Ventura‚ Rianna 2-13 Argumentative Essay: Additional fare‚ additional problem for students and parents The LRT and MRT fare hike has been the issue in the senate recently. It has been discussed that it is for the improvement of LRT and MRT operations. It is also for the additional budget for social services and infrastructure development. However‚ the 1.2 million daily passengers of the railway transit find it unfair. They are complaining about the negative effects of the hike
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other. You will find it interesting to note how many occupations‚ industries‚ and even countries are represented in this class. Since it is believed that most learning in an online environment occurs when learners engage in open dialogue to discuss and offer explanations to each other‚ you are likely to find that the diversity of experiences‚ cultures and backgrounds of your classmates will enhance the richness of your own learning and performance. Here’s how it works: 1. Go to the Discussion Board
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television personality and a fashion designer. She is a role model for many people and has maintained a good image as a person and is seen as a physical example of beauty. Jessica Simpson promote this brand of being effective‚ she claimed “I’m passionate about Proactiv because it works.” When you see Jessica promote a facial product like this then people are likely to want to try it to gain or maintain a pretty face like Jessica. The author of this Proactiv ad with Jessica Simpson was intended to take
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