Education Capacity Building Teachers Apply for Funding Nuffield Foundation » Teachers » Practical Biology » > Bio molecules » DNA » Extracting DNA from living things Practical Biology A collection of experiments that demonstrate biological concepts and processes. In partnership with Society of Biology logo Search Practical Biology go Society of Biology CLEAPSS Topics > Cells to systems > Energy > Control and communication > Exchange of materials > Bio molecules > Health and disease > Environment
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Extraction of Caffeine from Tea Leaves Santos‚ Raphael‚ Samson‚ Nonia Carla‚ *Tabora‚ Brylle‚ Tan‚ Kate Michal Department of Biological Sciences‚ University of Santo Tomas‚ Manila‚ Philippines Abstract Tea leaves are natural products that are harvested from agriculture. Caffeine‚ on the other hand‚ is a stimulant that has been shown to speed up the time of reaction‚ increase alertness‚ and improve one’s concentration. What we did in this experiment was to extract the alkaloid caffeine content
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Power of DNA Analysis In the world of medicine and disease there is one disease that is as well known as it is deadly: Cancer. Many people understand the severity of cancer and how it attacks the immune system and ultimately can kill a person. The war on cancer was started in 1971 by President Nixon and this set of four decades worth of study and has lead to a large amount of information being learned about the cancer genome. A new strategy has recently surfaced and is gaining ground‚ DNA analysis
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Abstract The purpose of this experiment was to study the transfer of genetic information on plasmid F’lac by using Escherichia coli. Plasmid transfer was measured by using two different methods. The first one was by using selection and contraselection with three antibiotics: streptomycin(which was replaced by naladixic acid for the second part of the experiment)‚ampicillin and kanamycin and the second one by using a colour indicator ( X-gal). As significant results‚ the percentage of transfer for
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1) Using tweezers remove individual plants from the soil and remove the roots with scissors. Measure the weight of each individual and place in a 1.5 mL microfuge tube. Place this tube directly into liquid N2 to freeze plant tissue. 2) Remove tubes from the liquid N2 and quickly grind the plant tissue with a pestle. Add 300 L of Methanol 1%HCl and continue to grind the plant tissue. Allow extraction to occur overnight in a dark refrigerator. 3) Add 200 L Milli-Q H20 to each tube. Next
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Identification of Mystery Plasmids Abstract Plasmids serve as important tools in genetics and biotechnology labs‚ where they are commonly used make many copies of a particular gene. The aim of this experiment was to identify three mystery plasmids based upon their characteristics; such as size‚ antibiotic resistance‚ lacZ profile and conjugative properties. The results obtained showed that plasmid number2 was the pDSK519 plasmid and its size was 26229.58 Bp. Plasmid pDSK519 also was found to be
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The Figure 4.20 shows the profile growth of Escherichia coli type K-12 which was cultured in 20 g/L of glycerol treated with supplements from a carbon source under anaerobic condition and the medium supplemented with 10 g/L of trypton. About 10% working volume of inoculums were added to the medium for the profile growth process. The effects on growth curves of strains of Escherichia coli were investigated by measuring the concentration of succinic acid produced and its optical density by using HPLC
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Extraction of Caffeine from Tealeaves Perez‚ Albert Solomon K.*; Quiambao‚ Marie Angela C;.Pascua‚ Hanna Harriette R.; Patricio‚ Maria Roxanne DC.; 2-Chemistry‚ Department of Chemistry‚ College of Science University of Santo Tomas España St.‚ 1015‚ Manila Abstract Caffeine is soluble in both water and organic solvents‚ solid-liquid extraction and liquid-liquid extraction were used in the experimentation process. Caffeine was extracted using hot water‚ however‚ due to its
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Extraction of Caffeine from Tea Leaves Department of Biological Sciences‚ College of Science University of Santo Tomas España‚ Manila‚ 1028 ABSTRACT: Caffeine is a white crystalline xanthine alkaloid that acts as a stimulant drug. Caffeine is found in varying quantities in the seeds‚ leaves‚ and fruit of some plants. It is most commonly consumed by humans in infusions extracted from the seed of the coffee plant and the leaves of the tea bush‚ as well as from various foods and drinks containing
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Evolution of Eukaryotic Cells from Prokaryotic Cells DNA- Deoxyribonucleic acid (DNA)‚ a self-replicating material present in nearly all living organisms‚ is the acid that carries genetic information. RNA- Ribonucleic acid (RNA)‚ is an acid that is present in all living cells‚ it’s main role is to act as messenger and carry instructions from DNA fro controlling the synthesis of proteins. What are Eukaryotic Cells and Eukaryotic Organisms? A eukaryotic cell is a cell that contains complex
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