Extraction of Caffeine from Lipton Yellow Label Teal Leaves Arlie Bamiano‚ Jealine Bernabe‚ Petrenne Caimbon*‚ Jhia Caso Department of Biology‚ University of Santo Tomas Abstract In order to extract pure caffeine from Lipton Yellow Label tea leaves‚ several extractions and phase transition techniques were employed to 6.5029 grams of sample. Initially‚ the tea leaves were boiled in water to extract tea from the leaves (Solid-Liquid Extraction). After extracting the tea‚ several steps of
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acetone as the solvent. The chlorophyll and carotenoid pigments were extracted by using column chromography and alumina was used as the solvent. Solvents of different polarities were used‚ starting with the least polar‚ to extract the certain components from the leaves. They were then analyzed by using thin- layer chromatography. Procedure: The first part of the experiment dealt with breaking down the spinach leaves in a mortar and pestle. Acetone was added to this to help with the breakdown of the
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Extraction of Caffeine from Thea sinensis Abstract Extraction of Caffeine from Thea sinensis main objective is to isolate‚ purify characterized caffeine from tea leaves. Sublimation technique was used to get the % yield which is 0.07%. The melting point of the standard caffeine with the sublimate is 229°C. Introduction The active ingredient that makes tea and coffee valuable to humans is caffeine. Caffeine is an alkaloid; a class of naturally occurring compounds containing nitrogen and
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Extraction of Caffeine from Tea Leaves Introduction Caffeine is soluble in boiling water and as a result it is easily extracted from tea bags by steeping in hot water. This process leaves behind the water insoluble portions of the tea bag. However‚ water extracts more than just caffeine‚ so a final separation is done with an organic solvent that will dissolve primarily caffeine. The organic solvent used in this experiment is Dichloromethane (CH₂Cl₂). Dichloromethane is less polar than water
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Long stands of double helical DNA can fit into the nucleus of a single cell because DNA is specially packaged through a series of compaction events to fit easily within cell nuclei. Even though the length of DNA per cell is about 100‚000 times as long as the cell itself‚ it only takes up only about 10 percent of the cell’s volume. The DNA molecule‚ in order to condense‚ wraps itself around groups of histone proteins‚ and then the chromatin folds back on it‚ nucelosomes pack together to create a compact
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DNA DNA‚ or Deoxyribonucleic Acid‚ is described‚ in Encarta Encyclopedia as a genetic material of all cellular organisms and most viruses. DNA carries the information needed to direct protein synthesis and replication. Protein synthesis is the production of the proteins needed by the cell or virus for its activities and development. Replication is the process by which DNA copies itself for each descendant cell or virus‚ passing on the information needed for protein synthesis. In most cellular
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EXPERIMENT 13： Extraction: Extraction with acid and alkali Objective 1. To recover the benzoic acid and p-dichlorobenzene from its mixture from its mixture by using acid-alkali extraction. 2. To determine the percentage recovery and melting point of the recovered benzoic acid and p-dichlorobenzene. Introduction Acid-base extraction is a process which purifying the acids and bases from mixtures based on their chemical properties. Acid-base extraction is performed to isolate the compounds and natural
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projects! Read a letter from Jimmy Wales and Michael Snow. | [Hide] [Help us with translations!] | DNA vaccine What is antisense technology? Antisense refers to opposing the normal order (“sense”) of the code in DNA. The DNA (deoxyribonucleic acid) in genes directs cells to assemble the proteins which comprise living creatures. The order of bases in DNA corresponds to the ordering of amino acids to form the proteins. To produce protein‚ the DNA of the genes in cells is first transcribed into
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Conclusions of DH5α DNA transformation with red colonies resistance to ampicillin and the lacZ gene Introduction: In this experiment‚ a plasmid with a gene that has resistance to the antibiotic ampicillin and has lacZ is used to transfer the resistance into E. coli bacteria in red colonies. This same technique is used to give diabetics their insulin‚ and to give dwarfs growth hormones. The point of this lab is to give the groups an idea how DNA can be transformed
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EGFP protein into pET41a(+) Plasmid Introduction The overall purpose of the experiments conducted is to test the creation of recombinant plasmid using recombinant DNA technology. The research of recombinant DNA began with the use of E.Coli (Escherichia coli)‚ a common bacteria found in the intestines of warm-blooded or- ganisms (5). Scientists worked together and generated a way‚ from cloning and using recombi- nant research‚ to achieve recombinant DNA. The gene that is the focus
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