February 2013 Critical Analysis of DNA Replication in Bacteria DNA replication is a biological process that occurs in all living organisms and copies their DNA; it is the basis for natural inheritance. The process starts when one double-stranded DNA molecule produces two identical copies of the molecule. The cell cycle (mitosis) also pertains to the DNA replication/reproduction process. DNA replication‚ in eukaryotes‚ is controlled within the context of the cell cycle. As the cell grows and divides
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1.0 TITLE Extraction of Phenol and Flavanoid compound of the edible brown seaweed species (Eucheuma Cottonii). 2.0 INTRODUCTION 2.1 An overview of Seaweed Macro-algae or “seaweeds” are multicellular plants growing in salt or fresh water. They belong to the lower plants‚ meaning that they do not have roots‚ stems and leaves. Instead they are composed of a thallus (leaf-like) and sometimes a stem and a foot (holdfast). Some species have gas-filled structures to provide buoyancy. They are often
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The effects of black tea on the growth of E. coli bacteria cultures Kierstin Barker‚ Melissa Bischak‚ Jackie Tyszkiewicz‚ Errin Enany September 25‚ 2012 Abstract: This study was carried out in order to investigate whether black tea has antimicrobial properties as stated in Steven Johnson’s The Ghost Map. If tea does have antimicrobial properties‚ then it could aid in warding off waterborne diseases. We believed that if black tea is steeped in boiled water‚ then the amount of bacteria exposed
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BIOTECHNOLOGY RECOMBINANT DNA technology 1. This is a modern biotechnological advance ‚ in which a desired gene fragment can be inserted in to a cloning vector and the resulting DNA (Recombinant DNA) can be amplified in suitable host. 2. A vector can be a plasmid‚ cosmid‚bacterophage‚retroviruses‚ animal and plant viruses or artificial chromosomes like YAC‚ BAC‚or HAC.(Yeast artificial chromosome‚ bacterial........) 3. The rec. DNA produced can be amplified or cloned in a suitable vector
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bacteria with pGLO plasmid Experiment #5 Aim: Purpose of this lab is to have plasmid activity transformed Material: Bacteria starter plate‚ pGLO DNA Plasmid‚ microcentrifuge tubes‚ Ice‚ water bath‚ CaCl2 Transformation solution‚ (LB) agar plate‚ (LB/Amp) agar plate‚ (LB/Amp/ara) agar plate‚ Micropipette‚ and Micropipette tips. Method: Genetic transformation is a procedure which is done by taking genes from one organism and putting them in another organism. A gene is a piece of DNA that instruct
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DNA Technology: Biotechnology 1) Selective breeding a. 2) Genetic engineering – humans tinker with organisms genes a. Cloning –take haploid cel and replace with a diploid nucleus (comes from organism whos traits you want to duplicate. b. Recombinant DNA – DNA from 2 or more sources. Done by Euk cells during Meiosis. Always from same molecule. 3) Biologists first started doing recombinant DNA from a prok cell and combined it with another prok cell because it was easier. 4) DNA from a
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DNA Isolation from Onion! Today‚ the students are going to isolate DNA from onions. You will need a blender or a food processor for this experiment. If you do not have one‚ check to see if your teacher does. If neither of you have one‚ then let me know ahead of time‚ and I can provide one from my home. You will also need to bring a knife and cutting board to cut the onions as well as a measuring cup. All other materials will be provided. The first part of the DNA experiment is to grind
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DNA and the Gene: Synthesis and Repair 1) Watson and Crick elucidated the structure of DNA in 1953. Their research built on and helped explain the findings of other scientists‚ including ________. A) X-ray diffraction studies by Rosalind Franklin and Maurice Wilkins. B) Chargaff’s rules: C = G and T = A. C) Scientists who recognized that a nucleotide consisted of a sugar‚ a phosphate‚ and a nitrogen-containing base. D) All of the above were important considerations in the elucidation of
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Introduction When extracting lipids or fats from foods‚ both the method as well as the solvents chosen to perform a complete‚ or close to complete extraction are important. If these two elements are not taken into consideration‚ the extraction may not be complete‚ or the extract may contain a large quantity of undesired impurities. The natural fats and oil are mixtures of glycerides of fatty acids. Fats and oils are naturally occurring organic compounds which belong to a large group of water insoluble
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Results and Discussion A. Table of Result of Extraction Weight (g) Tea Leaves 5.5783 Crude Caffeine 0.0246 Empty Beaker 117.5350 Beaker + Crude Caffeine 117.5596 The table above shows the weight of the sample tea leaves‚ the crude caffeine content in the sample‚ the empty beaker used in the extraction‚ and the beaker with the crude caffeine. It is induced from there that for that amount of sample tea leaves‚ which is equivalent to three bags of Nature’s Pride Tea; there is a 0.0246
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