amplicon‚ both digested wt-goi and mut-goi from other components. A purified digested gene product is more advantageous for the DNA ligation‚ in which the gene products with sticky ends will be inserted to a plasmid vector. Also‚ to transform E.coli DH5α cells by introducing the plasmids DNA which contains the gene of interests into the E.coli strain(DH5α). The plasmid DNA can replicate inside the transformed E.coli DH5α cells‚ only successful transformed cells can produce the protein that is resistance
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Topic: Concepts of DNA Fingerprint and Forensic Analysis Table of Contents Introduction………………………………………………………………………………. 3 What is DNA Fingerprint...................................................................................................3 Forensic Science…………………………………………………………………………..4 DNA Fingerprint Methods Restriction Fragment Length Polymorphism (RFLP)…………………………………...4 Polymerised Chain Reaction (PCR)……………………………………………………...5 Short Tandem Repeats
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Life of Escherichia Coli Escherichia Coli 0157:H7 bacterium can live in the intestines of cattle‚ therefore meat can become infected during slaughtering. You can contract this bacterium by eating undercooked meat‚ especially ground beef. Escherichia Coli 0157:H7 can also be contracted by eating contaminated fruits or vegetables‚ as well by drinking raw fruit juices or milk‚ or sewage contaminated water. Infection can also be spread via person to person. Escherichia Coli 0157:H7 can also be
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carrageenans are extracted from red seaweed and assume a gelatinous structure. [pic] Figure 1.Different types of carrageenan. They differ in structure‚ properties and uses. Production of carrageenan‚ particularly κ-carrageenan can be done in either of two ways. The first method‚ which is considered as the original one‚ involves the extraction of carrageenan into an aqueous solution‚ filtration to remove the seaweed residue and finally‚ the recovery of the carrageenan from the solution. Hence‚
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The investigation on the average mass of DNA with the mass of banana‚ strawberry and kiwi. Purpose: The purpose of this lab is to investigate the comparison between the amount of DNA per gram of fruit that can be extracted from a banana‚ strawberry and kiwi and to determine which one has more DNA. Hypothesis: The banana genome contains 837 MBPs and the strawberry genome contains 206 MBPs and the kiwi genome contains 128 MBPs. This states that there are more base pairs in a banana genome
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EXPERIMENT NO. 1 AIM: To perform isolation of the genomic DNA from the bacterial cell. REQUIREMENTS: Biological: Bacterial culture (DH5α) Chemical: Solution 1 - 10ml • Glucose (50mM) - 500μl • Tris-Cl (pH 8.0‚ 25mM) - 250μl • EDTA (pH 8.0‚ 10mM) - 200μ Solution 2 • SDS (1%) Phenol: chloroform: isoamylalcohol (25:24:1)‚ Absolute ethanol‚ 70% ethanol‚ Sterile distilled water Apparatus: Micropipettes‚ conical flask‚ measuring
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Recombinant DNA Technology Recombinant DNA technology refers to the ability to isolate specific DNA sequences and alter or manipulate them to produce desired effects. More often‚ recombinant DNA technology is referred to as biotechnology. Recombinant DNA technology is fascinating in that it has developed into a multi-billion dollar industry‚ and completely revolutionized agriculture and pharmaceutical industries‚ all within the past 50 years. According to one account‚ biotechnology was born
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Biology101 McDowall 10/29/2012 Escherichia Coli “The Bacterial Beast” E.coli or binomial name Escherichia coli was discovered by a German pediatrician named Theodore Escherich in 1885. Dr. Escherich originally named the bacteria‚ bacillus communis coli. After the demise of Dr. Escherich the intestinal bacteria was then named Escherichia coli after the late doctor in 1919. The bacteria Escherichia coli are classified as follows. Domain- Bacteria Kingdom- Eubacteria Phylum- Proteobacteria
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1 Automatic Keyphrase Extraction and Segmentation of Video Lectures Arun Balagopalan‚ Lalitha Lakshmi. B‚ Vidhya Balasubramanian‚ Nithin Chandrasekharan‚ Ashwin Damodar Amrita School of Engineering‚ Amrita Vishwa Vidyapeetham‚ Coimbatore‚ India Abstract—Keyphrases are essential meta-data that summarize the contents of an instructional video. In this paper‚ we present a domain independent‚ statistical approach for automatic keyphrase extraction from audio transcripts of video lectures
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Discussion: Extraction and crystallization- The purpose of this lab was to extract and crystallize compounds in a mixture using acids and bases. 1 gram of the unknown was dissolved into 30 mL of ethyl acetate. The mixture was then transferred to a separatory funnel. Using aqueous solutions of different pH we were able to separate organic acids‚ bases and neutral compounds from each other. Using three 10 mL portions of HCl‚ we reacted the HCl with the base in solution. This made it possible to
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