experiment‚ plasmid lux and a control plasmid (pUC18) will be introduced into E. coli by transformation. There are four basic steps to the procedure; Preparation of competent cells (These steps should be performed by the instructor.) 1. Place a vial of CaCl2 solution and the tube of E. coli in the ice bath. 2. Using a sterile pipet‚ transfer 590µL CaCl2 solution to the tube containing 50µL of the bacteria. 3. Tap the vial with the tip of your index finger to mix the solution. 4. Incubate the cells for at
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Methodology of DNA What is DNA? DNA was discovered by the Swiss biochemist‚ Johann Friedrich Miescher‚ in 1869‚ while he was working in Tubingen‚ Germany. He found that the DNA molecule is large; acidic in nature and rich in phosphorus‚ but only in the 1930s was the real and complex structure of DNA fully studied. DNA (deoxyribonucleic acid) is the genetic material in all prokaryotes and eukaryotes‚ i.e. it is the material responsible for the transfer of hereditary traits from parents to their
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Prokaryotic Cell Structure Bacterial Cell Structure Mariana Ruiz Villarreal/Wikimedia Commons Prokaryotes are single-celled organisms that are the earliest and most primitive forms of life on earth. As organized in the Three Domain System‚ prokaryotes include bacteria and archaeans. Prokaryotes are able to live and thrive in various types of environments including extreme habitats such as hydrothermal vents‚ hot springs‚ swamps‚ wetlands‚ and the guts of animals. Prokaryotic Cell Structure
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The cell is the fundamental structural unit of all living organisms. Some cells are complete organisms‚ such as the unicellular bacteria and protozoa; others‚ such as nerve‚ liver‚ and muscle cells‚ are specialized components of multi-cellular organisms. Cells range in size from the smallest bacteria-like mycoplasmas‚ which are 0.1 micrometer in diameter‚ to the egg yolks of ostriches‚ which are about 8 cm (about 3 in) in diameter. Although they may differ widely in appearance and function‚ all cells
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1 Detecting glowing E.Coli Colonies by making recombinant DNA from the lux operon of Vibrio Fischeri to pGEM. Liao‚ Tffany The marine bacterium Vibrio Fischeri produced bioluminescence effect due to lux operon transcription. The purpose of the experiment is to create a genomic library of Vibrio DNA and clone the lux operon by making Recombinant DNA and transform into another organism‚ E. Coli. Chromosomal DNA of vibrio fischeri was first extracted and digested with restriction enzyme Sal I‚ then
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the structure of DNA they wanted to use the information to help them identify how DNA is replicated. Three different theories of replication were proposed by Watson and Crick. The semi-conservative model‚ where the DNA strand splits into two halves‚ which will then create a new DNA strand consisting of the old original half and a new half. The conservative model where the whole of the original DNA strand acts as a template and is replicated to make a completely new strand of DNA. The dispersive model
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Homosexuality and DNA Honors Biology The human X and Y chromosomes have been completely sequenced. The X chromosome contains 153 million base pairs and harbors a total of 1168 genes. While the Y chromosome contains only 50 million base pairs and is estimated to contain about 251 genes. Educational institutions such as Baylor University‚ the Max Planck Institute‚ the Sanger Institute‚ Washington University in St. Louis‚ and others have spent countless hours and millions of research dollars
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day to us. From a large scale of things‚ down to the smallest and tiniest piece they explore all of these. One of these smallest things yet very interesting that we can sometimes never imagine of existing because we can’t see it through our naked eye that is bounded to all of us living things is the DNA. Every now and then‚ technology has been used in every aspect in our life. But we know that technology has its advantages and disadvantages. Recently it’s now being bounded with our DNA. DNA technology
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Department of Education Agusan National High School Caraga Administrative Region Division of Butuan City Antimicrobial Activity of Banana (Musa sapientum Linn.) Inflorescence Ethanolic Extract against E. coli and S. aureus Armona Jean B. Ongtico Mel Algil A. Agra Jerrson F. Oronan Researchers’ Mrs. Letecia S. Villanueva Research Coach Abstract The experience of human misery in the form of disease is perhaps as old as the inception
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The purpose of this experiment is to separate a solution of an acidic and organic compound and purify its respective acidic product through the techniques of an acid base extraction‚ recrystallization‚ and use of Melt Temp (melting point range). The reaction of an unknown acid (3-chlorobenzoic acid) with diethyl and sodium hydroxide was observed‚ and the solution naturally separated into an aqueous and organic solution. The mixture of acid and organic solution was created and then separated through
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