The Amplification DNA Extraction from minced meat samples using the Polymerase Chain Reaction (PCR) and Gel Electrophoresis for Purification of the DNA. Date: 14th/21st of October 2016 Partner(s): Aisling Loughman. Aim: The aim of the experiment is learn the technique to extract DNA using minced meat samples (Pork‚ Beef and mixed)‚ amplify the extracted DNA using the PCR Technique and further visualise the extracted DNA by Gel Electrophoresis under UV light. Introduction: “The method
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Unit 7 Introduction In this piece of coursework‚ there are few amounts of ideas and experiments that I could achieved of which different products to test for my concluding idea. The type of bacteria that I am going to discuss and chosen is E-coli. I will also going to research the effectiveness of antibacterial cleaning products‚ for instance sanitizer. I will also‚ research which is the most effective product for the house hold and some other work places. Background Information What are
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Extracting DNA from Human Cheek Cells: Conclusion The hypothesis of my experiment‚ if the strawberry DNA product was strandlike yet clumply then human epithelial cell DNA will be strandlike and clumpy too‚ was supported. This is because through the observations noted when the lab was being conducted‚ it can be seen that the human epithelical cell DNA was small‚ stringy‚ clumpy and squishy‚ just like the strawberry DNA product. Other observations of the human epithelial cell DNA was it was not only
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Abstract:Conjugation is a natural occurring process that involves the transfer of DNA from one cell into another through a physical connection between the cells. In the following experiment‚ two strains of Escherichia coli bacterial cells (donor F’lac+strs and recipient F-lac-strr) underwent conjugation to produce a transconjugant strain (F’lac+strr). MAC plates and streptomycin were utilized to determine if conjugation had occurred. When plated‚ the donor colonies appeared red and the recipient
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DNA Extraction Lab Purpose: To compare the amount of DNA extracted from two different species‚ despite using the same method. Hypothesis: I predict that the liver will produce a higher quantity of DNA than the strawberry. This is because I believe that animals have a higher DNA yield because our structure is more complex than a plant’s structure. Materials: -Sample of Strawberries -Zip lock bag -DNA extraction buffer -Cold ethanol -Glass rod -Double ply cheese cloth -Two test tubes
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Brandon Schmetterer 3-13-15 Biology labs DNA Extraction Lab DNA is extracted from humans for genetic testing‚ for body identification‚ and for analysis of forensic evidence. The first step of DNA extraction is to take cheek cells from the test subject. Next‚ the cells must be burst open in order to release DNA. Third‚ DNA is separated from protein and debris. Lastly‚ the DNA must be isolated. A buccal swab is necessary in order to collect the cheek cells .The micropipettes are used to add lysis solution
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suggestion offered by the manager of the restaurant is a validated idea that should garner the support from the ownership of the establishment. The manager acknowledges a pending threat to the business operations of the restaurant‚ and becomes very insightful by observing and theorizing a possible correlation between the decline of sales of the restaurant’s beef products and the increase of reporting of E. Coli contamination at local grocery stores. It will be imperative for the ownership to take up on this
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RESULTS AND DISCUSSION E. coli and S. aureus were tested to determine effectiveness of photodynamic inactivation with the use of methylene blue as photosensitizer and blue LED as alternative light source. Table 1 shows the resulting number of colonies for each replicate of every treatment per bacteria tested. Each of the treatments was averaged to get the mean number of colonies per treatment. All experimental setups were produced in quintuplicate‚ however only three of the five replicates were
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Escherichia coli also known as E. Coli‚ is a type of bacteria which thrives in polluted water. Many people will come in contact with this type of bacteria in their lifetime. In fact‚ E. coli is normally found in the intestine of humans and animal where it plays a significant role in the digestive system. The bacteria can also be found in raw meat and uncooked vegetables as well as polluted water. Most of the strain of E. coli are harmless therefore they will not cause illness if consumed. “One of
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1) TITLE (2pt): Testing antimicrobial props in soaps 2) INTRODUCTION (6 pts): In this experiment we wanted to test how effective different types of soaps against E. Coli. For this experiment we were interested in looking at antimicrobial soaps vs. non-antibiotics soaps in their effectiveness to kill E. Coli. The protocol that we used was the Kirby Bauer technique. This technique was used to test the antimicrobial properties of each soaps. (“Kirby-Bauer” 2015). 3) METHODS (6 pts): We first started
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