The purpose of the DNA extraction lab was not only to inform students on how DNA is present in humans and all organisms‚ but to also educate them on how DNA can be extracted using common household materials. Also‚ the lab was very efficient as it introduced the students to extracting their own DNA found on their cheek cells as well as letting them take an observation on how DNA appears or how it is formed. Additionally‚ students were instructed through a very clear and simple procedure‚ which guided
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Vol. 1 ACETIC ACID 115 ACETIC ACID 1. Introduction Acetic acid [64-19-7]‚ CH3COOH‚ is a corrosive organic acid having a sharp odor‚ burning taste‚ and pernicious blistering properties. It is found in ocean water‚ oilfield brines‚ rain‚ and at trace concentrations in many plant and animal liquids. It is central to all biological energy pathways. Fermentation of fruit and vegetable juices yields 2–12% acetic acid solutions‚ usually called vinegar (qv). Any sugar-containing sap or juice can
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Definition of Terms The following terms were defined for the readers to have a better understanding of the reserch paper. Active constituent. It refers to the component of the plant responsible for the exhibitory activity of the drug.1 Agar. It is gelatinous colloidal extractive of a red alga (as of the genera Gelidium‚ Gracilaria‚ and Eucheuma) used especially in culture media or as a gelling and stabilizing agent in foods.2 Agar-well diffusion method.3 It is a method of bioassay wherein
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DETERMINATION OF COPPER BY COMPLEXATION‚ SOLVENT-EXTRACTION AND SPECTROPHOTOMETRY ABSTRACT To determine the concentration of copper in an unknown solution by using copper complexation‚ solvent extraction and spectrophotometry. Standards are used to create a calibration curve and the unknown concentration of copper is then calculated by using the linear equation from the calibration curve. The concentration of copper in the unknown solution 201 was found to be 12.57± 0.25 μg/mL. INTRODUCTION Copper
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Common Aspects of Acid Prehydrolysis and Steam Explosion for Pretreating Wood Hans E. Grethlein Michigan Biotechnology Institute‚ PO Box 27609‚ Lansing‚ Michigan 48909‚ USA & Alvin O. Converse Thayer School of Engineering‚ Dartmouth College‚ Hanover‚ New Hampshire 03755‚ USA Abstract The initial rate of hydrolysis using cellulase from Trichoderma reesei for various wood samples is directly proportional to the surface area available to the enzyme. Both dilute acid hydrolysis in a continuous
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EXTRACTION OF INVERTASE FROM YEAST AND EFFECT OF pH ON ENZYMATIC ACTIVITY Jenelle C. Faustino‚ John Gambit B. Garcia‚ Fatima S. Jusay‚ Oliver Alexander B. Lao and Eunice L. Licudine Group 4 2 E Medical Technology Biochemistry Laboratory ABSTRACT Enzymes are substances that are produced by living organisms and act as catalysts in order to speed up or chance a chemical reaction without changing itself at the end of the reaction. Invertase was extracted first from baker’s yeast. Determination
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Surgical extraction I start by escorting the patient into the surgery I put full personal protective equipment on myself and the patient‚ I have placed all needed instruments on the work top for the dentist to access. Instruments: * Mirror‚ probe and tweezers * Local anaesthetic equipment * Aspirator and saliva ejector * Hand piece (fast slow and straight) and surgical burs * Scapel‚ periosteal elevator and swabs * Retractors * Forceps * Elevators * Bone forceps
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coffee. The average pure caffeine yield was 0.330% with a standard deviation of 0.179. This yield is slightly higher than the yield of this report because of the error that occurred during the experiment. A small amount of coffee from the first extraction was stuck in the nozzle of the separatory funnel so it was removed with a pipet and then decanted. This resulted in a loss of product. The average impure caffeine yield was found to be .948% with a standard
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Experiment title: Extraction of Bacteria Plasmid DNA and Analysis of extracted DNA Samples Objectives: 1) To study and understand the steps for extract bacteria plasmid DNA. 2) To measure the concentration and purity of extracted DNA by using spectrometric method and agarose gel electrophoresis method. 3) Determine the size of extracted DNA by using agarose gel electrophoresis method. Materials and Methods: (Refer to UDEE2124 lab manual from page
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Advantages: Sulfamic acid is the fastest de-scaler - It dissociates into hydroxonium ions more readily in aqueous solution than the others‚ therefore giving a greater concentration of atoms that are able to react with the calcium in lime scale. It is safe to use because it does not produce chlorine gas [5]‚ which can be toxic. Sulfamic acid also has a low volatility. Disadvantages: Sulfamic Acid can be an irritant to eyes or skin and is the most expensive of the de-scalers. Q2) How these de-scalers
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