3.2.4 Genomic DNA extraction of the root tips After the treatment of the onion bulbs with lake water sample at different period of time‚ the DNA from the root tips will be ready to be extracted and observed. First of all‚ the genomic of the Allium cepa will be easily done by the already available Uneasy® Plant Mini kit (Qiagen‚ Germany) in the laboratory. All of the procedures can be performed by referring to the manual provided by the kit. A very small amount of root tips that weigh 100 mg will
Premium DNA Bacteria Gene
Acetylsalicylic Acid/Aspirin and N-(4-hydroxyphenyl) Ethanimidate/Acetaminophen respectively from Excedrin. The first reaction scheme to undergo acid-base organic extraction was to isolate the most acidic compound in Excedrin with a weak base‚ K2HPO4. We isolated the aspirin using aqueous base extraction‚ with Dipotassium phosphate then treated with HCl to force acidification. The mechanism for Scheme 1 is shown below: The second reaction scheme we used was also an organic acid-base extraction involving
Premium Aspirin Acetic acid Salicylic acid
Intact Dilation and Extraction Intact dilation and extraction which is also known as intact dilation and evacuation‚ intrauterine cranial decompression and most commonly but controversially known as partial birth abortion has created ethical waves in medicine over the years. This specific procedure has been a primary focus of many politicians and religious groups although it only makes up approximately .08% of abortions‚ or approximately 1‚032 per year‚ based on research done in the year 2006
Premium Pregnancy Abortion Fetus
---------------------------------- Acidspunk installation instruction ---------------------------------- Acidspunk requires an MMX processor and DirectX Place the .dll in winamp’s plugin directory. Start winamp and press "ctrl-k" Choose acidspunk and press "configure" (or "alt-k") Set the screen resolution Start the plugin "ctrl-shift-k" Check the framerate by pressing F2 while the plugin is running. The plugin should run between 20-30 frames per second to look good. If not adjust
Premium Linear interpolation Display resolution Interpolation
Studying the pH of Strong Acid‚ Weak Acid‚ Salt‚ and Buffer Solutions The purpose of the current experiment was to determine the pH of various hydrochloric acid and acetic acid solutions‚ to determine the pH of various salt solutions‚ to prepare a buffer solution‚ and determine the effects of adding a strong acid and strong base to the buffer solution versus adding a strong acid and strong base to water. The measured pHs for the hydrochloric acid solutions were 1.6‚ 2.2‚ 2.9‚ and 3.8. The measured
Premium PH Hydrochloric acid Acid dissociation constant
DNA is a double helix molecule that contains information that is used to make up a person’s body. DNA controls every aspect of a person’s body from their eye and hair color‚ height‚ and other features. DNA’s specific and unique characteristic can be crucial when solving a crime. DNA can be used to convict a suspect or exonerate an innocent person. When DNA is found it is even more important that is handle properly to ensure proper identification and accuracy of testing. The evolution of DNA technology
Free DNA
TITLE/PROBLEMS * Can peanut butter with seaweed extract be a main ingredient in making poisons for rats? * “Peanut-Weed Arsenic Poison” * “Peanut-Weed Rat Killer” RRS Why Do Poisons Matter? “Long after the 1962 publication of Rachel Carson’s book‚ Silent Spring‚ and the subsequent birth of the environmental movement‚ the days of concern over the effects of at-home and commercial pesticide use are long from over. Carson’s book described numerous environmental impacts of indiscriminate
Free Pesticide Environmentalism DDT
Acid-Base extraction theory states that when an acid reacts with an organic base it results in a salt that is water-soluble and a neutral molecule that is insoluble. The addition of an acid to a mixture containing acids and bases will result in the acid remaining unchanged and the base reacting to form a salt. The results from the experiment were fairly consistent when separating the strong acid‚ weak acid‚ or the base. In part one of the experiment‚ you had to separate the strong acid. When added
Premium Acid Chemistry Base
Acid Rain Introduction: Titrations are often recorded on graphs called titration curves‚ which generally contain the volume of the titrant as the independent variable and the pH of the solution as the dependent variable (because it changes depending on the composition of the two solutions). The equivalence point on the graph is where all of the starting solution (usually an acid) has been neutralized by the titrant (usually a base). One can easily find the pKa of the monoprotic acid by finding
Premium Acid dissociation constant PH Acid
BEHAVIOUR OF AMINO ACIDS IN SOLUTION Abstract In this practical the acid-base behaviour of amino acids was assessed. Amino acids are amphoteric. They have the basic (amine) and the acidic (carboxylic) functional groups. These show the same type of equilibrium reactions that all weak acids and bases undergo‚ and the relative amount of each can be altered by adjusting the pH of the solution. For this practical glycine was used as a model to show this unique nature of amino acids. A series of
Premium Amino acid Acid