November 5‚ 2014 Bioinformatics Leigh Ann Santana DNA Extraction Lab DNA extraction is an important process because the DNA first needs to be purified away from proteins and other cellular contaminants. Cell are needed‚ because that is where the DNA is located. Inside almost every cell in our bodies is a nucleus‚ and inside each nucleus is about two meters of DNA. The following steps are needed to purify DNA from a cheek swab. Collect cheek cells‚ Burst cells open to release DNA‚ separate DNA
Free DNA Cell Gene
Experiment 3 Liquid-Liquid Extraction Discussion The success of extracting each compound out of the solution can be seen in the IR graphs for each substance. For ethyl 4-aminobenzoate‚ the N-H‚ C=O and C-O are distinguishable in their proper wavenumber positions. The amino N-H stretch was found between 3224 cm-1 and 3423 cm-1. The C=O was found at 1681 cm-1 and the C-O was at 1280 cm-1. The IR for benzoic acid also displays its significant bonds‚ O-H and C=O. The O-H was between 2566 and
Premium Solubility Chemistry
Chamberlin Date: 29 April 2017 Experiment Title: Acid-Base Titration Abstract: Vinegar is a common household item containing acetic acid as well as some other chemicals. This experiment is designed to determine the molar concentration of acetic acid in a sample of vinegar by titrating it with a standard solution of NaOH. CH3COOH(aq) + NaOH(aq) -> CH3COONa(aq) + H2O(l) By adding the sodium hydroxide‚ which is a basic solution‚ to the acetic acid‚ which is an acidic solution‚ a neutralization
Premium Titration
10-fold dilution of this ionic iron solution and 5 drops of concentrated HNO3 was made. This solution served as the source of ionic Iron for the remainder of the lab and was labeled “stock ionic Iron solution.” Next‚ a 50-mL aqueous ionic Iron and FerroZine® complex solution was prepared by adding 5.00mL stock ionic Iron‚ 3-mL of acetic acid buffer‚ 2-mL of 5% hydroxylamine hydrochloride‚ allowing five minutes for hydroxylamine to reduce Fe3+ to Fe2+‚ adding 2.5-mL of 0.01 M FerroZine® solution‚ and
Premium Chemistry Titration Water
Determination of Ka for a Weak Acid Introduction In the experiment preformed the objective is to titrate a weak acid with a strong base. In a titration of a weak acid with a strong base the titrant is the strong base and the analyte is a weak acid. The reaction that will occur is the direct transfer of protons from the weak acid to the hydroxide ion. The data gathered will be represented on the titration curve‚ a graph of the volume of titrant being the strong base plotted against the pH .The
Premium Acid Base Acid dissociation constant
Beakers15 cm Cheesecloth1 Resealable Bag1 Rubber Band (Large. Contains latex pleasewear gloves when handling if you have a latex allergy).Standing Test TubeWooden Stir StickFresh‚ Soft Fruit (e.g.‚ Grapes‚ Strawberries‚ Banana‚ etc.) ScissorsDNA Extraction SolutionIce Cold EthanolYou Must ProvideContains sodium chloride‚ detergent and waterFor ice cold ethanol‚ store in the freezer 60 minutes before use. Procedure If you have not done so‚ prepare the ethanol by placing it in a freezer for approximately
Premium Solubility DNA Water
acetic acid present in commercial vinegar and the weight-to-volume percent of ammonia present in window cleaners. Acid-base titrations can be conducted using the above samples against standardized aqueous NaOH and standardized aqueous HCl solutions respectively. 2. Introduction: Acetic acid‚ commonly known as ethanoic acid CH3COOH‚ is found in commercial vinegar. It is a weak organic acid that gives vinegar its characteristic pungent smell‚ sour taste and slight acidity. Being an acid‚ vinegar
Premium Acetic acid Titration Acid
In order to find the Vitamin C content you will need to use a process called titration which‚ according to the Columbia Electronic Encyclopedia‚ is when you add a chemicals to a liquid in order to create a reaction which can determine the content of a substance inside the liquid. This is how you will find the Vitamin C content of different juices. Iodine will be added to the juice‚ which will create a reaction that can help determine the Vitamin C content. To start‚ pour 30 milliliters (mL) of Lugol’s
Premium Iodine Titration Starch
Lab Report: Acid-Base Titration Brianna Morrison Chemistry 111 October 11‚ 2010 Aim: To standardize a solution of the base sodium hydroxide using oxalic acid dihydrate as primary standard acid. Also to determine the amount of sodium hydroxide it takes to titrate a weighted sample of an unknown acid. Procedure: As outlined in instructions provided‚ no changes were made to the procedure. Data: Part A: Trial 1: Amount of H C O 2H O: 0.96 g Buret before titration: 3.6
Premium Sodium hydroxide Base PH indicator
Ashley Spees CHM 261- Batzer Chem Final Write Up- Kool Aid Lab Total Acid This part of the lab requires titration of Kool Aid with an NaOH solution. The reason titrations are used is to determine the concentration of an unknown solution by reacting a strong acid with a strong base. Titrations are hard to accomplish‚ though‚ due to the fact that indicators used to show the endpoints are very sensitive and one drop could make the solution titrate past its endpoint. Blue stock solution: 3 mg/L
Premium Sodium hydroxide Base Titration