take spinach leaves and extract the chlorophyll and carotenoid pigments by using acetone as the solvent. The chlorophyll and carotenoid pigments were extracted by using column chromography and alumina was used as the solvent. Solvents of different polarities were used‚ starting with the least polar‚ to extract the certain components from the leaves. They were then analyzed by using thin- layer chromatography. Procedure: The first part of the experiment dealt with breaking down the spinach leaves
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& E6 Extraction of Chlorophyll from Fresh Spinach and Investigation of the Photochemistry of Chlorophyll Chlorophyll a Chlorophyll b E5 - Extraction of Chlorophyll from Fresh Spinach E6 - Investigation of the Photochemistry of Chlorophyll The aim of this experiment is to investigate the photochemistry of chlorophyll. This experiment will be performed in two lab periods. In the first lab period you will extract chlorophyll‚ the green pigment in leaves‚ from spinach. In the
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Chemistry Laboratory I Experiment #6 - Column and Thin-Layer Chromatography (TLC) of a Spinach Extract Pre-Lab Assignment (1) List 5 ways that TLC can be used in an organic chemistry lab experiment. To identify an unknown‚ to monitor the course of a reaction and assess the purity of its product‚ to determine the best solvent for a column chromatography separation‚ to determine the somposition of each fraction from a column chromatography separation‚ and to determine whether a substance purified by
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Title of Experiment Extraction of Spinach Date that the Experiment was Performed This experiment was performed on Wednesday‚ September 17th‚ 2014 at 2:45 pm in the St Ignatius Science Center Laboratory 323. Partners Names Taylor Jackson and Matt D’Angelo. Taylor‚ Matt‚ and I shared the same data. Purpose/Goals/Objectives The purpose of this experiment was for each student to use column chromatography to separate plant pigments from spinach leaves. Some goals and objectives were to
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Isolation‚ purification and characterization of alkaloid caffeine from tea leaves Date Submitted: September 3‚ 2014 Abstract Caffeine is one of the main constituents of tea leaves. In this experiment‚ 0.0039 g of pure caffeine was isolated from tea leaves. This was done through solid-liquid extraction followed by multiple liquid-liquid extraction. Then crude material obtained was then purified by sublimation. The isolated caffeine was subjected to thin layer chromatography (TLC) together
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Extraction of Caffeine from Tea Leaves Marc Tugaoen‚ Kristine Vanzuela‚ Rafael Villanueva‚ Justeen Wong Department of Chemistry‚ University of Santo Tomas‚ Manila‚ Philippines Abstract This experiment has been divided into 4 set-ups‚ first was the solid-liquid extraction‚ next was the liquid-liquid extraction‚ then the sublimation and last was the melting point determination. The solid-liquid and liquid-liquid extraction were both done during the first meeting‚ the DCM layer was filtered and
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Abstract This work describes the extraction of caffeine from tea leaves to determine its % caffeine. The extraction process selectively dissolves one or more compounds in a mixture into an appropriate solvent. In this experiment‚ it was visible in the process wherein the components of the tea leaves were dissolved in two solvents‚ water and dichloromethane (DCM)‚ with DCM used for multiple extractions. The organic layer was evaporated and the determined % caffeine was 0.12%. Furthermore‚ the purified
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Extraction of Caffeine from Tea Leaves Department of Biological Sciences‚ College of Science University of Santo Tomas España‚ Manila‚ 1028 ABSTRACT: Caffeine is a white crystalline xanthine alkaloid that acts as a stimulant drug. Caffeine is found in varying quantities in the seeds‚ leaves‚ and fruit of some plants. It is most commonly consumed by humans in infusions extracted from the seed of the coffee plant and the leaves of the tea bush‚ as well as from various foods and drinks containing
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Extraction of caffeine from Lipton tea leaves Maria Gianna Beatrice L. Cancio*‚ Joe Mari Isabella B. Caringal‚ Rowena A. Chiang‚ Patricia Deanne del Valle Department of Biological Sciences‚ College of Science‚ University of Santo Tomas‚ España‚ Manila‚ Philippines Abstract Caffeine was extracted from Lipton tea leaves by multiple extraction techniques namely solid to liquid extraction and liquid to liquid extraction; the purification by sublimation and melting point determination. Three
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EXTRACTION OF CAFFEINE FROM TEA LEAVES ABSTRACT Caffeine extraction from the commercial tea leaves (Lipton Yellow Label Tea) that was done is multiple extraction. 4 tea bags were used in the experiment; tea leaves were weighed having 8.5333 grams. The leaves were boiled in a beaker with 150ml of water; the aqueous tea extract was transferred in a seperatory funnel mixed with DCM (20ml)‚ done thrice. The DCM layer was washed with 20ml 6M NaOH in the seperatory funnel; the organic layer
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