Of a Sample Mixture By Liquid-Liquid Extraction Reading assignment: Techniques in Organic Chemistry 2nd ed pages 75-99. 3rd ed pages 113-140. Topics and Techniques i) identification of solvent layers of two immiscible solvents ii) partioning of a compound between two immiscible solvents and determination of KD iii) liquid-liquid extraction with aqueous acids and bases with organic solvents. iv) use of drying agents Introduction Liquid-liquid extraction is a method used for the separation of
Premium Solubility Acetic acid Solvent
JUICE EXTRACTION After weighing‚ sugarcane is loaded by hand or crane onto a moving table. The table carries the cane into one or two sets of revolving knives‚ which chop the cane into chips in order to expose the tissue and open the cell structure‚ thus readying the material for efficient extraction of the juice. Frequently‚ knives are followed by a shredder‚ which breaks the chips into shreds for finer cane preparation. The chipped (and shredded) cane then goes through the crusher‚ a set of roller
Premium John Stuart Mill Sugar The Table
DNA Extraction from Fruit 1. What was the purpose of adding liquid soap and salt in step #1 and how does NaCl contribute to maximum DNA extraction. The purpose of using soap was to destroy the membranes inside a kiwi cell. Soap helped with that because it dissolves the membranes easily. Salt or NaCl was used to remove proteins and carbohydrates. NaCl caused the proteins and carbohydrates to precipitate. 2. Why was it necessary to “mush” the kiwi by hand? If the step was omitted‚ what
Free DNA Cell Cell membrane
Extraction and Analysis of Plasmid DNA from E. coli Cells Introduction A plasmid is an extra-chromosomal element‚ often a circular DNA. Since a plasmid is by definition an extra-chromosomal element‚ it cannot make use of any origin of DNA replication in a chromosome (BP site). Meaning that DNA synthesis within a plasmid depends on having an origin of DNA synthesis of its own. Plasmids are often found in bacterial cells‚ in which they are used as transfer agents for transmitting various antibiotic
Premium Molecular biology DNA Plasmid
Extraction of DNA Molecule of an Onion In an Aqueous Solution By: Introduction DNA(deoxyribonucleic acid) is found in every living organism. It provides the information and instructions to build and regulate cells in organisms. The information it contains is used when organisms are being reproduced. It is made up of two polynucleotide chains known as DNA strands. Within the two chains there are four nucleotide units: adenine‚ thymine‚ cytosine‚ and guanine. Materials and Methods An onion
Premium DNA Organism Biology
Black Garlic Omelet and Spinach Waking up in the morning and eating a delicious cheesy omelet sounds like a perfect morning breakfast. And with this wonderful and healthy omelet recipe‚ you will be packed with energy to last the whole day! Yields: 3 Servings Ingredients: 3 tbsp. of Cheddar Cheese 3 Eggs 1 clove of Black Garlic 3 tbsp. of Parmesan Cheese ¼ cup of Tomatoes ¼ cup of Green Onion A pinch of salt A pinch of Pepper 1 cup of Spinach Some Olive Oil/ Cooking Spray Method of Preparation:
Premium Food Milk Egg white
2.3.3 Feature Extraction Feature extraction deals with the extraction of the distinctive features out of face images so that those face images can be differentiated among each other. There are several algorithms available for extracting features out of a face image. The most common is the use of mathematical formulas that generate a mathematical representation of a face image that is termed as a template‚ these templates are “the refined‚ processed and stored representation of the distinguishing
Premium Data Algorithm Data mining
the quantification of albumin and casein were performed and analyzed after extraction of the said proteins from their respective sources. Isolation of proteins was initiated by the breakage of the cell wall / membranes in three different ways. Homogenization of invertase‚ albumin and casein were achieved via grinding process‚ addition of 1M acetic acid and acidification by 0.1M hydrochloric acid correspondingly. Extraction of invertase and casein involved precipitation through the utilization of 95%
Premium Protein
Experiment 3 Liquid-Liquid Extraction Discussion The success of extracting each compound out of the solution can be seen in the IR graphs for each substance. For ethyl 4-aminobenzoate‚ the N-H‚ C=O and C-O are distinguishable in their proper wavenumber positions. The amino N-H stretch was found between 3224 cm-1 and 3423 cm-1. The C=O was found at 1681 cm-1 and the C-O was at 1280 cm-1. The IR for benzoic acid also displays its significant bonds‚ O-H and C=O. The O-H was between 2566 and
Premium Solubility Chemistry
Abstract The experiment‚ entitled Extraction and Characterization of Proteins‚ aims to isolate casein from milk and albumin from egg; to explain the methods employed for protein extraction; to apply spectrophotometric methods in characterizing and quantifying extracted casein and albumin. The experiment was divided into 2 parts; the extraction of Albumin from egg and the determination of protein concentration via the Warburg-Christian method and Bradford Assay method. In the first part‚ egg
Premium Protein Amino acid Acid