The purpose of this experiment is to separate a solution of an acidic and organic compound and purify its respective acidic product through the techniques of an acid base extraction‚ recrystallization‚ and use of Melt Temp (melting point range). The reaction of an unknown acid (3-chlorobenzoic acid) with diethyl and sodium hydroxide was observed‚ and the solution naturally separated into an aqueous and organic solution. The mixture of acid and organic solution was created and then separated through
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AN INVESTIGATION OF PHOTOSYNTHETIC ELECTRON TRANSPORT OF CHLOROPLASTS FROM _SILVERBEET LEAVES_ Introduction Photosynthesis is the process by which plants use the energy from sunlight to produce sugar‚ which cellular respiration converts into ATP‚ the "fuel" used by all living things. The conversion of unusable sunlight energy into usable chemical energy is associated with the actions of the green pigment‚ chlorophyll. Light Dependent Reactions are the initial stage of photosynthesis‚ in which solar
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EXPERIMENTAL PROCEDURE: 1. 2.07 g of the mixture of naphthalene and benzoic acid was weighed accurately on a balance. The mixture was then dissolved in 40 ml dichloromethane and the solution was poured into a separatory funnel. 2. 20 ml of aqueous sodium bicarbonate solution was added to the dichloromethane. A stopper was placed on the separatory funnel and was shaken and pressure was frequently released. The 2 liquids separated into clear layers and the lower layer (dichloromethane layer) was
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SOP: Solid phase extraction of cylindrospermopsin in filtered water samples Document identifier: SOP_TOXIC_UDU_05F Prepared by: James S. Metcalf and Geoffrey A. Codd‚ UDU Date: 7 July 2005 1 Introduction In order to determine the concentration of cylindrospermopsin in the extracellular fraction of filtered environmental waters‚ solid phase extraction (SPE) is necessary to concentrate the toxin to concentrations capable of being detected by HPLC. 2 Experimental 2.1 Materials
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Introduction Extraction is a purification technique used in organic chemistry to separate compounds from a mixture of two or more compounds. There are three different extraction techniques: liquid-liquid extraction‚ solid-liquid extraction and chemically active extraction. All three types of extraction follow the same principle. Organic molecules dissolve in organic solvents and polar molecules dissolve in aqueous solvents. This phenomenon is observed because of the intermolecular forces between
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Berry DNA Extraction Lab I. Problem Statement: Successfully extracting the DNA from strawberries. II. Background info: DNA is the genetic information in organisms that codes for proteins‚ and controls the cells function. The DNA is a double helix molecule that is formed by various nucleotides which include Adenine‚ Thymine‚ Guanine‚ and Cytosine with sugar phosphate. Different organisms can have larger or smaller genes. DNA is also what makes up chromosomes during cell replication. Strawberries
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the basic methods of procedure that can be used for later experiments. This experiment involves the separation of benzoic acid from a more crude form‚ consisting of benzoic acid‚ methyl orange‚ a common acid/base indicator‚ and cellulose‚ a natural polymer of glucose (Huston‚ and Liu 17-24). The technique that is used to perform this separation is called extraction. Extraction is a systematic process of separating mixtures of compounds‚ taking advantage of the affinity differences of compounds to separate
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Surgical extraction I start by escorting the patient into the surgery I put full personal protective equipment on myself and the patient‚ I have placed all needed instruments on the work top for the dentist to access. Instruments: * Mirror‚ probe and tweezers * Local anaesthetic equipment * Aspirator and saliva ejector * Hand piece (fast slow and straight) and surgical burs * Scapel‚ periosteal elevator and swabs * Retractors * Forceps * Elevators * Bone forceps
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p-dichlorobenzene from its mixture using acid-alkaline extraction. 2. To determine the percentage recovery and melting point of benzoic acid and p-diclorobenzene. Apparatus:Separatory funnel(250mL)‚Buchner funnel‚beaker. Materials:Benzoic acid‚p-dichlorobenzene‚ether‚10% NaOH‚conc.HCl‚distilled water‚ anhydrous CaCl2. Introduction: Organic compounds in an aqueous mixture can be separated by shaking the aqueous solution with a solvent which is immiscible with water. Extraction involves dissolving
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Maternity leave Introduction At present‚ almost every country has provisions for maternity leave that provided to the female employees. Most industrialized countries have some form of maternity or parental leave policy that effectively grants employment protection to women around childbirth and in some cases to either parent for an even longer period after childbirth (Puhani and Sonderhof‚ 2009). Maternity leaves are the temporary leaves that allowed by the company or institutions to a female
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