DNA extraction lab 1. A number of steps are required to isolate DNA from cellular content. Describe what happens at each step‚ and why it acts to separate the parts of the cell. The steps include a) breaking cell open to release the DNA; b) separating the DNA from cellar materials and proteins; c) using alcohol to precipitate the DNA; d) cleaning the DNA; e) confirming the presence of the DNA. a) Breaking cell open to release the DNA: the cells are separated from each other by physical means such
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Distillation is one of the most common separation techniques used in laboratories or industries where liquid chemicals are separated based on their volatilities. Distillation is usually done by vaporizing the liquid mixture in one vessel and condensing the vapors into another. The liquid mixture being distilled is called distilland and the resulting liquid is called distillate. In laboratories‚ there are several types of distillation used according to the needs such as simple distillation which involves
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Experiment D: Separation of Alcohols by Fractional Distillation Pre-lab Properties: Propanol‚ butanol‚ methanol Purpose: To separate two alcohols by distillation and analyze results with GC. Do not plug mantle directly into electrical outlet‚ use controller! Use 40/60 mol percent propanol/butanol (no cyclohexane or toluene are used in this lab because they are more toxic) Set up fractional distillation Set heat at highest level to start‚ then turn back down to 60-80% when vapor starts up column
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CHEMICAL ENGINEERING LABORATORY PROJECT TITLE: SOLID LIQUID EXTRACTION OF GANODERMA LUCIDUM NAME: WIJETUNGE MUDALIGE DINITHI ISHARA WIJETUNGE ID NO: 007118 SUPERVISOR: DR SIVAKUMAR MANICKAM GROUP MEMBERS: KOW NIEN WEI AAZRA OUMAYYAH PANKAN JASON HEW YIP SENG DATE: 30 APRIL 2012 H83 CEL Chemical Engineering Laboratory Solid Liquid Extraction of Ganoderma Lucidum Author: W.M.Dinithi Ishara Wijetunge I certify that: (i) Apart from the experimental results reported in the lab notebook which
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Date Performed: December 17‚ 2013 Group No.: 8 Date Submitted: January 6‚ 2014 Experiment 4: Extraction and Chromatic Separation of Plant Pigments from Tomato Paste ABSTRACT In column chromatography a mixture is dissolved in a solvent and poured over a column of solid adsorbent then eluted with the same or a different solvent. This method is often used for preparative
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SEGi University Extraction of Benzoic Acid by Balvinder Singh (SCM 015 677) Lab Partners: 1) Surrean Rao 2) Wong Kai Jun 3) Kanaga Luckshimi 4) Sasiskala Regal ECE2332 Chemical Engineering Practices Submitted: 1st October 2013 Supervisor: Mr Ayman William 1.0 Abstract /summary The purpose of this experiment to carry out in the lab is because to determined the capability to water droplet to carry out the liquid-liquid extraction. The studies is to determine
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DNA Extraction Lab Purpose: To compare the amount of DNA extracted from two different species‚ despite using the same method. Hypothesis: I predict that the liver will produce a higher quantity of DNA than the strawberry. This is because I believe that animals have a higher DNA yield because our structure is more complex than a plant’s structure. Materials: -Sample of Strawberries -Zip lock bag -DNA extraction buffer -Cold ethanol -Glass rod -Double ply cheese cloth -Two test tubes
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Journal of Food Engineering 95 (2009) 240–253 Contents lists available at ScienceDirect Journal of Food Engineering journal homepage: www.elsevier.com/locate/jfoodeng Review Application of supercritical CO2 in lipid extraction – A review F. Sahena a‚ I.S.M. Zaidul a‚*‚ S. Jinap a‚ A.A. Karim b‚ K.A. Abbas a‚ N.A.N. Norulaini c‚ A.K.M. Omar b a b c Faculty of Food Science and Technology‚ Universiti Putra Malaysia‚ 43400 UPM Serdang‚ Selangor D.E.‚ Malaysia School of Industrial Technology‚ University
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Lab 5 Acid/Base Extractions Introduction The purpose of this experiment is to separate either the organic base (amine) or organic acid (carboxylic acid) from a mixture that contains inorganic impurities (salt) by performing a liquid-liquid extraction and then taking a melting point. Key Experimental Details and Observations Our starting material‚ Compound B‚ was a fine white powder and weighed 0.535g. The final product was a shiny white sheet that resembled acrylic paint and weighed 0.109g
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