Abstract The purpose of this experiment was to perform a liquid-liquid extraction method to extract the caffeine from the tea bags that were provided‚ and then recrystallize the caffeine. The solvents used in the experiment were an aqueous sodium carbonate and dichloromethane (DCM). Anhydrous calcium chloride pellets were used to dry the solution and emulsion layer and the DCM was then decanted. After washing the anhydrous calcium chloride pellets with more DCM‚ the solvent was evaporated‚ leaving
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Unit Test Science V I. Match the animals with how they get and eat their food. Write only the letter. 1. Snake a. sucking blood with their tiny tubes 2. Frog b.using beaks to get their prey 3. Birds c. coiling their bodies to their prey 4. Lion d. catching insects with their sticky tongue 5. Mosquitoes e. tearing meat with their strong pointed teeth B. 1. mammals a. they have dry scaly skin 2. birds b. they have mammary gland that produce milk to feed
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Procedure 2: DNA Extraction from Cheek Cells Materials: Water‚ Clear Dish Soap‚ Table Salt‚ Isopropyl Alcohol (70%) or Ethanol‚ Food Coloring 1. To 200 Ml drinking water add two teaspoons of salt 2. Gargle the salt water for 1 minute. 3. Spit the gargled water into a beaker (or new cup). Now your cheek cells are suspended in the salt water. 4. Gently stir the salt water with one drop of soap (try to avoid air bubbles) 5. In a separate beaker (or cup)‚ mix 20 ml isopropyl alcohol and 1-3 drops
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DATE: September 16‚ 2012 TO: Patricia Bennett‚ Supervising Principal FROM: Connor Sims‚ Associate SUBJECT: Oil Drilling & Gas Extraction Industry in the US Analysis (21111) This report presents information regarding the industry‚ the primary operator of oil and gas field properties. The industry fuels its key buyers‚ the Natural Gas Distribution (22121) and the Petroleum Refining (32411) industries‚ with crude oil and natural gas. The industry continuously battles
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We wanted to extract‚ see and analyze DNA from a single strawberry( 12.11 g). The long‚ thick fibers of DNA store the information for the functioning of the chemistry of life. DNA is present in every cell of plants and animals. The DNA found in strawberry cells can be extracted using common‚ everyday materials. To start the procedure we needed the following supplies; one strawberry‚ one plastic cup‚ 10 ml of Cell Lysis Buffer (10% detergent‚ 1% NaCl)‚ one plastic zip-lock bag‚ one coffee filter‚
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Micro-extraction of urine and/or stomach contents and analysis by TLC Abstract Thin Layer Chromatography (TLC) is one of the oldest chromatographic techniques used for the identification of compounds and for determination of the presence of trace impurities. The aim of the experiment was to determine two unknown samples (X and Y) by comparing results to the known standard drugs‚ of which there was five‚ using a specific TLC method. The same technique was used for the determination of
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Describe an alternative method for evaporation of the CH2Cl2 Can be steamed and then rinsed with ethyl acetate for several hours‚ and then rinsed with water‚ or can be soaked in a bath of CO2 and run through water‚ making carboxylic acid. (2) Caffeine: (4) Repeated exposure can produce general deterioration of health by an accumulation in one or many human organs. First Aid Measures: Eye contact - Immediately flush eyes with plenty of water for at least 15 minutes. Cold water may be used. WARM
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NBPF: Value Extraction from Different Market Segments Tamiko Okada entered the back office of Nippon Batting Practice Facility (NBPF) in the Northern district of Tokyo‚ to contemplate her fledgling company’s marketing strategy. A decorated former collegiate softball player with a graduate degree from an American university‚ Tamiko had a deep knowledge of the game and of her customers. She lacked a marketing background‚ however‚ so she had recently signed up for a hosted CRM service that would allow
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Extraction of Caffeine from Tea Leaves using Dichloromethane (CH2Cl2) Abstract The extraction of caffeine (C8H10N402) was done in two parts. The first procedure used was solid-liquid extraction which was done by boiling the tea leaves in 100 ml. distilled water for 5-10 minutes. The filtrate was acquired and was extracted again by liquid-liquid extraction using dichloromethane (CH2Cl2). The combined DCM (CH2Cl2) was washed and dried to yield crude caffeine. The crude caffeine was purified
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Extraction and Analysis of Plasmid DNA from E. coli Cells Introduction A plasmid is an extra-chromosomal element‚ often a circular DNA. Since a plasmid is by definition an extra-chromosomal element‚ it cannot make use of any origin of DNA replication in a chromosome (BP site). Meaning that DNA synthesis within a plasmid depends on having an origin of DNA synthesis of its own. Plasmids are often found in bacterial cells‚ in which they are used as transfer agents for transmitting various antibiotic
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