Isolation of plasmid DNA and analysis of isolated plasmid Introduction: A plasmid is an autonomously replicating extra-chromosomal genetic element. In other words‚ this is a DNA molecule external to the bacterial chromosome that is able to replicate on its own and distribute its daughter molecules to daughter cells. You have successfully cloned a fragment of chromosomal DNA containing a tetracycline resistance cassette into a plasmid (pET11a). To this end you have (1) isolated total chromosomal
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INTRODUCTION Plant pigments consist of four main chemicals. These are Chlorophylls‚ The carotenoids‚ Anthocyanin and Betacaine. The aims of this experiment is to separate the plant pigment into different colours using solvents and column chromatography. The hypothesis of this experiment is that this plant pigment from spinach will contain the chlorophyll pigment or may contain the others too. Chlorophyll: Chlorophyll a is the main component of the plant pigments. This is used for the most
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Objective: The purpose of this lab is to allow you to become familiar with a procedure for extracting DNA‚ collecting DNA samples‚ and to observe the physical characteristics of DNA Background: The wild strawberry is a diploid because it only has two sets of chromosomes. While the grocery store kind is an octoploidy because it has eight sets of chromosomes. The reason we chose the grocery store strawberry is because we’d be able to extract more DNA. Ripe strawberries make enzymes which help break
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Isolation of Acetyleugenol (Clove Oil) from Cloves Purpose In this experiment‚ you will obtain the essential oil from cloves by steam distillation. You will then separate a component (acetyleugenol) from the oil by liquid-liquid extraction. Finally‚ you will isolate the acetyleugenol by using a rotovap for reduced pressure distillation‚ which will remove the organic solvent (dichloromethane). Background Whole cloves contain between 14% and 20% by weight of essential oils‚ but
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patient. However‚ we are only interested in the patient’s head and more specifically the brain‚ that is why in this step we perform an orientation step to orient all the brain images into a common coordination and then we perform the brain extraction using brain extraction tool (BET) which will result two images‚ one image contains only the brain and the other one contains the brain and the skull‚ both images are needed for further analysis‚ Figure 2.4 and Figure 2.5 Show the MRI image before and after
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dental patients reach peak candidacy for wisdom tooth extraction. This oral surgery procedure is best performed at this age because the wisdom teeth are fully formed‚ and removing them can prevent overcrowding and other dental issues from occurring down the road. The family dentistry professionals at Lowitz & Meier in Cincinnati‚ OH‚ will assess your teen’s wisdom teeth to determine whether extraction is the best option for them. Wisdom tooth extraction is a common form of oral surgery that minimizes
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Exp. 2 Separation of Three Compounds by Extraction Introduction: The purpose of this experiment is do demonstrate the proper procedures of extraction and to develop a practical understanding of this method. In this experiment‚ extraction is used to separate a known acid‚ base‚ and neural compound from each other. The first two compounds undergo proton transfer reactions in the presence of acids or bases. Acids RCOOH‚ like benzoic acid‚ was deprotonated to form RCOO-. Bases RNH2‚ like ethyl 4-aminobenzoate
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chemical synthesis: extraction of crude caffeine pose little toxicity to human health and the environment.(1) Q2) Things not green: According to GCP# 5 i.e. Safer solvent and auxiliaries: In liquid-liquid extraction of caffeine‚ Dicholoromethane was used which is a hazardous chemical and have potential acute health effects(2). According to GCP#12 i.e. Inherent safer chemistry for accident prevention: The separatory funnels used in the experiment for liquid-liquid extraction was a dangerous procedure
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limited and things were not advanced. However‚ the invention of the steam engine by James Watt in the late 19th century sparked an industrial revolution. It was a major turning point because it allowed for expansion
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RAJESH BUSINESS INTELLIGENCE GENERIC EXTRACTION CHAPTER – 4 EXTRACTIONS It is mainly used for extracting the data form other Source Systems like R/3 etc. DataSource: Defines as extract structure and transfer structure from source system (like SAP R/3) and transfer structure from BW side. ES -> What data to be extracted from source system TS -> What data to be extracted from source system to BW. TS is a group of fields which indicated how the data is coming from the source system
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