microfuge tube. Place this tube directly into liquid N2 to freeze plant tissue. 2) Remove tubes from the liquid N2 and quickly grind the plant tissue with a pestle. Add 300 L of Methanol 1%HCl and continue to grind the plant tissue. Allow extraction to occur overnight in a dark refrigerator. 3) Add 200 L Milli-Q H20 to each tube. Next‚ go to the fume hood and add 500 L of chloroform to each tube. Spin the tubes in a centrifuge set at the highest rpm for 2-5 minutes. 4) Return to
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There has been much debate in recent years among politicians‚ military officials‚ and civilians on whether or not the U.S. should be fully withdrawn from Afghanistan‚ regardless of the country’s economic and military capabilities. There has also been a proposed timetable‚ which President Obama says is in effect and currently being executed‚ to have up to ninety percent of our military personnel out of the country by no later than 2014. It is imperative that we extract our forces out of Afghanistan
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Extraction of Caffeine from Tealeaves Perez‚ Albert Solomon K.*; Quiambao‚ Marie Angela C;.Pascua‚ Hanna Harriette R.; Patricio‚ Maria Roxanne DC.; 2-Chemistry‚ Department of Chemistry‚ College of Science University of Santo Tomas España St.‚ 1015‚ Manila Abstract Caffeine is soluble in both water and organic solvents‚ solid-liquid extraction and liquid-liquid extraction were used in the experimentation process. Caffeine was extracted using hot water‚ however‚ due to its
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Laboratory #16: Analysis of Grease in Water by Solvent Extraction Experiment Date: 2/19/10 Due Date: 2/26/10 II. Objective The goal of this lab was to determine the amount of impurity‚ in the form of oil‚ in water using the method of solvent extraction‚ also known as liquid-liquid extraction‚ a method of separating two materials with different physical and/or chemical properties. Two methods of evaporation will be used to finally determine the concentration of impurity- one using an explosion-proof
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Extraction of Caffeine from Lipton Yellow Label Teal Leaves Arlie Bamiano‚ Jealine Bernabe‚ Petrenne Caimbon*‚ Jhia Caso Department of Biology‚ University of Santo Tomas Abstract In order to extract pure caffeine from Lipton Yellow Label tea leaves‚ several extractions and phase transition techniques were employed to 6.5029 grams of sample. Initially‚ the tea leaves were boiled in water to extract tea from the leaves (Solid-Liquid Extraction). After extracting the tea‚ several steps of
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How to make pencils? Background One of the oldest and most widely used writing utensils‚ the pencil originated in pre-historic times when chalky rocks and charred sticks were used to draw on surfaces as varied as animal hides and cave walls. The Greeks and Romans used flat pieces of lead to draw faint lines on papyrus‚ but it was not until the late 1400s that the earliest direct ancestor of today’s pencil was developed. About one hundred years later graphite‚ a common mineral occurring
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Abstract This work describes the extraction of caffeine from tea leaves to determine its % caffeine. The extraction process selectively dissolves one or more compounds in a mixture into an appropriate solvent. In this experiment‚ it was visible in the process wherein the components of the tea leaves were dissolved in two solvents‚ water and dichloromethane (DCM)‚ with DCM used for multiple extractions. The organic layer was evaporated and the determined % caffeine was 0.12%. Furthermore‚ the purified
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The First Experience for a Wisdom Tooth Extraction Just a year ago‚ I got my wisdom tooth pulled for the first time. My wisdom tooth had started to ache on last New Year ‘s day. Normally‚ I celebrate New Year’s day with special food‚ but I was no time for that because of an excruciating pain. Unfortunately‚ my husband and I didn’t know a good doctor in this city‚ and we couldn’t contact friend who knows well regional information because it was a big holiday! At the long time‚ I found a dentist
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Extraction of Caffeine from Thea sinensis Abstract Extraction of Caffeine from Thea sinensis main objective is to isolate‚ purify characterized caffeine from tea leaves. Sublimation technique was used to get the % yield which is 0.07%. The melting point of the standard caffeine with the sublimate is 229°C. Introduction The active ingredient that makes tea and coffee valuable to humans is caffeine. Caffeine is an alkaloid; a class of naturally occurring compounds containing nitrogen and
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Extraction of Caffeine from Tea Leaves Introduction Caffeine is soluble in boiling water and as a result it is easily extracted from tea bags by steeping in hot water. This process leaves behind the water insoluble portions of the tea bag. However‚ water extracts more than just caffeine‚ so a final separation is done with an organic solvent that will dissolve primarily caffeine. The organic solvent used in this experiment is Dichloromethane (CH₂Cl₂). Dichloromethane is less polar than water
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