Experiment 2 Titles Extraction of Bacterial Plasmid DNA and Analysis of Extracted DNA Samples Objectives * To learn the procedures needed in extracting the bacterial plasmid DNA * To determine the concentration of original DNA sample and purity of prepared DNA sample by using spectrophotometer * To analyze the extracted DNA sample by gel electrophoresis Materials and methods (Refer to UDBB2144 Laboratory 2A Manual Principles of biotechnology page 6-10) Results
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We wanted to extract‚ see and analyze DNA from a single strawberry( 12.11 g). The long‚ thick fibers of DNA store the information for the functioning of the chemistry of life. DNA is present in every cell of plants and animals. The DNA found in strawberry cells can be extracted using common‚ everyday materials. To start the procedure we needed the following supplies; one strawberry‚ one plastic cup‚ 10 ml of Cell Lysis Buffer (10% detergent‚ 1% NaCl)‚ one plastic zip-lock bag‚ one coffee filter‚
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DNA Extraction Lab 1/17/14 Purpose : This lab was conducted in order to show and analyze the way DNA is extracted. Hypothesis: If the lab is conducted properly then we should be able to view a visible amount of DNA from the strawberry and detergent mixture. Variables: The independent variable in this experiment is the strawberry mixture while the dependent
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Procedure 2: DNA Extraction from Cheek Cells Materials: Water‚ Clear Dish Soap‚ Table Salt‚ Isopropyl Alcohol (70%) or Ethanol‚ Food Coloring 1. To 200 Ml drinking water add two teaspoons of salt 2. Gargle the salt water for 1 minute. 3. Spit the gargled water into a beaker (or new cup). Now your cheek cells are suspended in the salt water. 4. Gently stir the salt water with one drop of soap (try to avoid air bubbles) 5. In a separate beaker (or cup)‚ mix 20 ml isopropyl alcohol and 1-3 drops
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Onion From Wikipedia‚ the free encyclopedia Jump to: navigation‚ search "Onions" redirects here. For the surname‚ see Onions (surname). This article is about the plant term. For other uses‚ see Onion (disambiguation). Onion Onions Scientific classification Kingdom: Plantae Division: Angiosperms Class: Monocots Order: Asparagales Family: Alliaceae Genus: Allium Species: A. cepa Binomial name Allium cepa L. Allium cepa var. proliferum‚ Top Onion The onion
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The Onion’s mock press release about the MagnaSoles shoe inserts satirizes how products are marketed to customers. The article is riddled through with malapropisms‚ and the whole article is a parody of an advertisement. Sarcastic irony is used when statements are made in the article that are obviously meant to show that the opposite is true‚ and hyperbole‚ or exaggeration‚ is used to show how advertisers will try to convince people that their product is useful for many more issues than it is.
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the Extraction lab is to separate and purify benzoic acid‚ 2-naphthol‚ and naphthalene. These organic solids are purified by partitioning the solid in 2 immiscible solvents‚ diethyl ether and sodium bicarbonate or sodium hydroxide. II. Significance a. The significance of the Extraction lab is to purify Acetanilide benzoic acid‚ 2-naphthol‚ and naphthalene by determining the partition coefficient. This value is determined by dividing the solubility of the given solute in the extraction solvent
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Extraction Separation of a Carboxylic Acid‚ a Phenol‚ and a Neutral Substance Purpose The objective of the experiment is to identify the three substances in the mixture and to determine the percent recovery of each from the mixture. The unknown will consist of either benzoic acid or 2-chlorobenzoic acid‚ 4-t-butyl phenol. Determine the weight of crystals and also the percent yield Introduction There are various techniques of extractions‚ solid/liquid extraction‚ which
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Extraction and Analysis of Plasmid DNA from E. coli Cells Introduction A plasmid is an extra-chromosomal element‚ often a circular DNA. Since a plasmid is by definition an extra-chromosomal element‚ it cannot make use of any origin of DNA replication in a chromosome (BP site). Meaning that DNA synthesis within a plasmid depends on having an origin of DNA synthesis of its own. Plasmids are often found in bacterial cells‚ in which they are used as transfer agents for transmitting various antibiotic
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of Onions”‚ you will read about the ways that you can impact the flavors and textures of different types of onions ranging from the more pungent to the milder. Bomze makes clear the two basic types of onions to look for when choosing a pungent or mild onion at the store. Shopping tips are included on how to check the neck‚ firmness‚ and thickness of skin when searching for a milder or more pungent onion. You will also learn in this article how to work with and control the flavor of the onion by
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