Strawberry DNA Extraction Donovan Roberts Mrs. Caudill Honors Biology 3/7/11 Introduction The structure of DNA is made of nitrogenous bases‚ phosphate groups‚ and sugars. These three parts of DNA form hydrogen bonds and create a right-handed double helix. The location of DNA in a eukaryotic cell is found within the nucleus‚ bound to proteins. DNA can be extracted from within the cell. They are several steps you must take to retrieve DNA from within the cell. When extracting DNA cells
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DETERMINATION OF COPPER BY COMPLEXATION‚ SOLVENT-EXTRACTION AND SPECTROPHOTOMETRY ABSTRACT To determine the concentration of copper in an unknown solution by using copper complexation‚ solvent extraction and spectrophotometry. Standards are used to create a calibration curve and the unknown concentration of copper is then calculated by using the linear equation from the calibration curve. The concentration of copper in the unknown solution 201 was found to be 12.57± 0.25 μg/mL. INTRODUCTION Copper
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Experiment 2: Acid- Base Extraction Separation of an Organic Acid‚ a Base and a Neutral Compound Introduction: The extraction technique is often used in the organic lab to separate organic compounds from mixtures. One type of an extraction technique is the acid-base extraction‚ which is used to separate organic compounds from a mixture based on their acid-base properties.1 There are certain principles that must be taken into account when choosing a solvent system to perform an extraction. The solvents should
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Theoretically‚ the spinach extract at 18oC should have a faster rate of reaction than the 5oC extract because higher temperature allows substrates to move more rapidly hence increases the chance of collision with the enzymes. Moreover‚ the experiment was low in accuracy as
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Tan 1 DNA EXTRACTION Aim : To extract the DNA from an egg yolk using various enzymes and to compare with other groups the most effective way to extract DNA. Hypothesis : To be able to observe white springy substances after mixing with enzyme and alcohol. Apparatus : -Test tube‚ spatula‚ glass rod‚ dropper‚ beaker‚ test tube rack‚ skewer. Materials : - 1 egg‚ meat tenderizer‚ salt‚ water ‚ soap‚ isopropyl alcohol 91%‚ pineapple juice. Variables : Manipulated Variable : Responding
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TAMARINDUS Linnaeus pp. 434-436‚ Manila * Srivastava‚ P. & Malviya‚ R. (2011) Indian Journal of Natural Products and Resources Vol. 2 (1) Sources of pectin‚ extraction and its applications in pharmaceutical industry. pp. 10-18 * May‚ C. D. Handbook of Hydrocolloids pp. 169-188 * Huang‚ J. M. G. Improved method for the extraction of Pectin‚ PO Box 4200 Highstown‚ New Jersey 08520 Acknowledgement I would like to thank God‚ for His guidance throughout this investigatory project. To my
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DNA from various fruits using cell disruption and separation techniques. Materials Used: 2 heavy duty zip-lock baggie 1 strawberry (fresh or frozen and thawed) 1 banana half 10 ml DNA extraction buffer* 2 Coffee filters Ice cold 95% ethanol 1 small beaker 2 Test tubes Wooden coffee stirrer *To make the extraction buffer‚ 100 ml of shampoo (without conditioner) was mixed with 15 g of NaCl and 900 ml of water. Procedures Used in Experiment Once all materials were gathered by group members‚ each
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Objectives 1. To separate a mixture of an acid and a neutral compound into its component by extraction. 2. To determine the melting point and the yield of the benzoic acid and the unknown sample. 3. To determine the unknown sample whether is is Trimethylmethanol or 1‚2‚4‚5-Tetrachlorobenzene. Introduction Extraction is a process of transferring a solute from one solvent to another. It is usually used to separate one or more components from a mixture. Diethyl ether‚ dichloromethane
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Anza Thomas 10/24/2013 CHE 261 (KC) Dr . McAtee Lab : Extraction of Caffiene Purpose: the purpose of this experiment is to extract caffeine from tea using solvent extraction techinques. Reference: Williamson‚ K.; Minard‚ R.; Masters‚ K. Macroscale and Microscale Organic Experiments‚ 2007‚ p. 137 to 171. Procedure: In a 50 mL beaker place 15 mL of water‚ 2.0059 g of sodium carbonate‚ and a wooden boiling stick. Bring the water to a boil on the ceramic heating plate. Remove the beaker
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1. Introduction Nepal has more villages than cities and towns. More than 80% of the people are dependent on agriculture for their livelihood. Nepal is rich in forests. % of the total land is covered with forest. Majority of the rural households are subsistence farmers and rely heavily on forest products to meet their daily requirements. Fuel wood is used for cooking and heating purposes‚ fodder and grass for livestock‚ leaf litter for manure‚ and timber for constructing houses and for making agricultural
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