We wanted to extract‚ see and analyze DNA from a single strawberry( 12.11 g). The long‚ thick fibers of DNA store the information for the functioning of the chemistry of life. DNA is present in every cell of plants and animals. The DNA found in strawberry cells can be extracted using common‚ everyday materials. To start the procedure we needed the following supplies; one strawberry‚ one plastic cup‚ 10 ml of Cell Lysis Buffer (10% detergent‚ 1% NaCl)‚ one plastic zip-lock bag‚ one coffee filter‚
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Micro-extraction of urine and/or stomach contents and analysis by TLC Abstract Thin Layer Chromatography (TLC) is one of the oldest chromatographic techniques used for the identification of compounds and for determination of the presence of trace impurities. The aim of the experiment was to determine two unknown samples (X and Y) by comparing results to the known standard drugs‚ of which there was five‚ using a specific TLC method. The same technique was used for the determination of
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Describe an alternative method for evaporation of the CH2Cl2 Can be steamed and then rinsed with ethyl acetate for several hours‚ and then rinsed with water‚ or can be soaked in a bath of CO2 and run through water‚ making carboxylic acid. (2) Caffeine: (4) Repeated exposure can produce general deterioration of health by an accumulation in one or many human organs. First Aid Measures: Eye contact - Immediately flush eyes with plenty of water for at least 15 minutes. Cold water may be used. WARM
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A Spectrophotometric Analysis of the Absorption of Green Light Versus Red Light Absorption in Spinach Leaves The goal of the experiment was to determine if green light had less ability to absorb than red light in spinach leaves. This was done by separating the photosynthetic pigments (chlorophyll a‚ chlorophyll b‚ carotene and xanthophylls) from one another using paper chromatography. The separated pigments were then analyzed for their absorption spectrum using a spectrographometer. When the
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NBPF: Value Extraction from Different Market Segments Tamiko Okada entered the back office of Nippon Batting Practice Facility (NBPF) in the Northern district of Tokyo‚ to contemplate her fledgling company’s marketing strategy. A decorated former collegiate softball player with a graduate degree from an American university‚ Tamiko had a deep knowledge of the game and of her customers. She lacked a marketing background‚ however‚ so she had recently signed up for a hosted CRM service that would allow
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Extraction of Caffeine from Tea Leaves using Dichloromethane (CH2Cl2) Abstract The extraction of caffeine (C8H10N402) was done in two parts. The first procedure used was solid-liquid extraction which was done by boiling the tea leaves in 100 ml. distilled water for 5-10 minutes. The filtrate was acquired and was extracted again by liquid-liquid extraction using dichloromethane (CH2Cl2). The combined DCM (CH2Cl2) was washed and dried to yield crude caffeine. The crude caffeine was purified
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Extraction and Analysis of Plasmid DNA from E. coli Cells Introduction A plasmid is an extra-chromosomal element‚ often a circular DNA. Since a plasmid is by definition an extra-chromosomal element‚ it cannot make use of any origin of DNA replication in a chromosome (BP site). Meaning that DNA synthesis within a plasmid depends on having an origin of DNA synthesis of its own. Plasmids are often found in bacterial cells‚ in which they are used as transfer agents for transmitting various antibiotic
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Experiment 2 Titles Extraction of Bacterial Plasmid DNA and Analysis of Extracted DNA Samples Objectives * To learn the procedures needed in extracting the bacterial plasmid DNA * To determine the concentration of original DNA sample and purity of prepared DNA sample by using spectrophotometer * To analyze the extracted DNA sample by gel electrophoresis Materials and methods (Refer to UDBB2144 Laboratory 2A Manual Principles of biotechnology page 6-10) Results
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“EXTRACTION AND QUANTIFICATION OF BRAIN TUMOR FROM MRI IMAGE” CHAPTER 1 INTRODUCTION 1.1 GENERAL: The principle task of our project is to recognize a tumor and its quantifications from a particular MRI scan of a brain image using digital image processing techniques and compute the area of the tumor by fully automated process. In medical imaging‚ segmentation of images plays a vital role in stages which occur before implementing object recognition
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Acid-Base Extraction: Separation of an Organic Acid‚ Base and a Neural Compound Introduction/Background Acid base extraction is a widely used commercial method in separating acids‚ bases and neutral compounds. This is concept is based on several physical properties of the compounds‚ such as boiling point‚ melting points and solubility. The different solubility properties of the protonated and non-protonated forms of these compounds depend on the functional groups present‚ such
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