Biotechnology Lab Report Lab: Extracting DNA from Bananas and Strawberries Purpose: To properly and successfully extract DNA from various fruits using cell disruption and separation techniques. Materials Used: 2 heavy duty zip-lock baggie 1 strawberry (fresh or frozen and thawed) 1 banana half 10 ml DNA extraction buffer* 2 Coffee filters Ice cold 95% ethanol 1 small beaker 2 Test tubes Wooden coffee stirrer *To make the extraction buffer‚ 100 ml of shampoo (without conditioner) was mixed
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separate a mixture of an acid and a neutral compound into its component by extraction. 2. To determine the melting point and the yield of the benzoic acid and the unknown sample. 3. To determine the unknown sample whether is is Trimethylmethanol or 1‚2‚4‚5-Tetrachlorobenzene. Introduction Extraction is a process of transferring a solute from one solvent to another. It is usually used to separate one or more components from a mixture. Diethyl ether‚ dichloromethane (methylene chloride)‚ trichloromethane
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261 (KC) Dr . McAtee Lab : Extraction of Caffiene Purpose: the purpose of this experiment is to extract caffeine from tea using solvent extraction techinques. Reference: Williamson‚ K.; Minard‚ R.; Masters‚ K. Macroscale and Microscale Organic Experiments‚ 2007‚ p. 137 to 171. Procedure: In a 50 mL beaker place 15 mL of water‚ 2.0059 g of sodium carbonate‚ and a wooden boiling stick. Bring the water to a boil on the ceramic heating plate. Remove the beaker from the hot plate and remove
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manure‚ and timber for constructing houses and for making agricultural implements. Poor people are more benefitted from forests products as they are not able to afford for alternative source of energy for fuel‚ timber etc. But studies also show that forest resources are equally consumed by other groups of people as well. Total land covered by forest has been declining rapidly from the last decade. Therefore forest resource management has been important. In Nepal‚ as in other developing countries
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Experiment title: Extraction of Bacteria Plasmid DNA and Analysis of extracted DNA Samples Objectives: 1) To study and understand the steps for extract bacteria plasmid DNA. 2) To measure the concentration and purity of extracted DNA by using spectrometric method and agarose gel electrophoresis method. 3) Determine the size of extracted DNA by using agarose gel electrophoresis method. Materials and Methods: (Refer to UDEE2124 lab manual from page 7 to
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What is a Tooth Extraction? A tooth extraction is the process of removing a tooth from the mouth. The procedure may need to be done for a number of reasons. Sometimes before getting braces‚ a tooth needs to be removed in order for the teeth to have enough room to move into their proper location and order for a straight smile. In other cases‚ a tooth may need to be removed because it has decayed or has become loose due to gum disease. Removing it is the first step in stopping the pain and infection
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Kadijat‚ and Kharla Ms. Bulahan Writer: Djina Jan-18-15 Lab #6 - DNA Extraction lab Introduction: DNA is a double stranded macromolecule composed of nucleotide bases pairing Adenine with Thymine and Cytosine with Guanine. S ince DNA is the blueprint for life‚ every living thing contains DNA. The extraction of DNA from cells and its purification are of primary importance to the field of biotechnology and forensics. Extraction and purification of DNA are the first steps in the analysis and ma
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It takes a period of 20 years for the Prunus Africana to grow to 30 meters or more. That is one reason there are only a limited number of Prunus Africana found in Kenya‚ Uganda‚ and the Democratic Republic of Congo. The extraction of pulverized‚ dark bark of Prunus Africana is the reason for high demand on these trees for production of drugs for prostate issues. The Prunus Africana is currently being harvested out of eastern highlands of Kenya in the Aberdares Mountains
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Acme Minerals Extraction Company A long time ago‚ work used to be assigned only to one person and that person was in charge of doing only that task. Then‚ with the new technology innovations‚ we start seeing how companies started to trained some employees to be what they usually called “multi-tasking employees”. Since technology kept changing and became more advanced companies realized that having “multi-tasking employees” was not enough to satisfy the market demands and the production problems
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DNA extraction lab 1. A number of steps are required to isolate DNA from cellular content. Describe what happens at each step‚ and why it acts to separate the parts of the cell. The steps include a) breaking cell open to release the DNA; b) separating the DNA from cellar materials and proteins; c) using alcohol to precipitate the DNA; d) cleaning the DNA; e) confirming the presence of the DNA. a) Breaking cell open to release the DNA: the cells are separated from each other by physical means such
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