8/12/2013 Intro I am going to investigate a factor that affects the rate of enzyme activity What are enzymes and how do they work? Enzymes are biological catalysts - catalysts are substances that increase the rate of chemical reactions without being used up. Enzymes are also proteins and thus have a specific shape. They are therefore specific in the reactions that they catalyse - one enzyme will react with molecules of one substrate. The site of the reaction occurs
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Sonia St James Investigation: Factors that affect the outcome of enzyme activity Introduction In this project I will monitor the rate of activity of Catalase. Catalase is an Enzyme which in the right conditions catalyses the decomposition of Hydrogen Peroxide into water and oxygen; 2H2O2 + Catalase >>> 2H2O + O2 Catalase is found in all cells and protects them from Hydrogen Peroxide which is a dangerous waste product that needs to be eliminated. Without Catalase living things could not survive
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Lab Report Factors That Affect Enzymes Reaction Rate Name of lab: Effects of temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration on Enzymatic Activity Introduction: Enzymes are the most important types of proteins‚ they act as catalysis (speed up chemical reactions). If enzymes didn’t exist‚ biochemical reactions would act to slowly and they couldn’t keep up with the metabolic functions. Enzymes have a three-dimensional structure that is really complex. This structure consists
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Enzymes are catalytic proteins that selectively speed up chemical reactions without by consumed by the reaction itself (1). Enzyme activity is significantly affected by factors such as temperature‚ substrate concentration‚ enzyme concentration and pH. Enzymes are highly specific and only catalyse one specific chemical reaction‚ speeding up the reaction by lowering the activation energy‚ the energy required to start a chemical reaction (2). At high temperatures and with various mechanical forces or
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1.7 Factors that affect the activity of an enzyme It is important when working with enzymes to understand basic enzymatic theory behind them when selecting conditions to measure the activity of the enzymes. The factors that are known to affect the concentration of enzymes are temperature‚ pH‚ concentration of enzyme‚ concentration of substrate‚ buffer type and concentration‚ the presence of any inhibitors and cofactors (Worthington-biochem.com). 1.7.1. Temperature With most catalysed reactions‚
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Factors Affecting the Rate of Enzyme Activity Justin Hunter G. Kim September 16‚ 2011 September 26‚ 2011 Abstract Catalase is a common enzyme found in nearly all living organisms that is frequently used by cells to rapidly catalyze the decomposition of hydrogen peroxide into less reactive oxygen and water molecules. Catalase is a protein that is most commonly found in the liver. The purpose of this experiment was to determine the effect that changes in temperature and pH have on the function
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How is pH affecting catalase activity? Hypothesis The catalase activity is assumed to be the most active in pH7. The higher or lower concentration away from the optimum pH of catalase‚ the slower the rate of activity is. Data Collection & Processing I collected the results of pH4‚ pH7‚ pH10 and pH13 after 2 minutes‚ and I repeated the experiment 3 times. Below is a table to show the results‚ their averages and standard deviations: | 1st time (sec) | 2nd time (sec) | 3rd time (sec)
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Enzyme Catalase Activity in Reaction with the Substrate Hydrogen Peroxide Abstract We performed these experiments to observe the effects of enzymes on the rate of reactions. We tested and compared the activity of the enzyme catalase on the substrate H2O2 in various states and percentages‚ and observed the absorption values of the enzyme-substrate relationship at different concentrations. Our results show that the more substrate available‚ the quicker the reaction will happen except in one test
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that the enzyme concentration will affect the rate of reaction but only up until a certain point because there is a limited supply of substrate. Enzymes are protein molecule that acts as biological catalyst by increasing the rate of reactions without changing the overall process. They are long chain amino acids bound together by peptide bonds. Enzymes are seen in all living cells and controlling the metabolic processes in which they converted nutrients into energy and new cells. Enzymes also help
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My lab group studied the effect of PH on reaction rate/ enzyme activity measured by foam height. PH is the measure of the concentration of hydrogen ions in a solution. The higher the hydrogen ion concentration‚ the lower the pH. Every enzyme has an optimal PH‚ meaning they have a very small window in which they are most active. Our enzyme (potato smoothie) had an optimal PH of 7.0-7.5. We know this because we measured the enzyme’s reaction rate by measuring foam height. The largest foam height we
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