Lab Report Enzymes (must be typed) Name: Amanda Gallegos Date: 2/15/15 Section: BIOL 101GL What is the Hypothesis of this experiment? (3 points) -The highest temperature will affect the reaction rate of enzymes. What is the dependent variable? (1 point) -Change of rate/product/color/absorbency. What is the independent variable? (1 pt) -The temperature. Explain in detail the procedure that you followed (including amount of substrate‚ enzyme etc‚ and the whole procedure including incubation times)
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In the early 1980’s the economy experiences a recession. A recession is “a significant decline in economic activity spread across the economy‚ lasting more than a few months‚ normally visible in real income‚ employment‚ industrial production‚ and wholesale-retail sales (Recession‚ Retrenchment‚ and Recovery: State Higher Education Funding & Student Financial Aid‚ 13).” Technically there were two recessions‚ the first was from January through July of 1980‚ and the second was from July 1981 through
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62 Iodine test for starch Amount of starch remaining Enzyme activity level Dark blue-black All None (0) Blue Most Low (1) Light brown Some Moderate (2) Gold None High (3) Part 1: Effect of Enzyme Concentration 1. Label five test tubes 1-5. Place 4 mL of 1 % starch in each of the first four test tubes. Place 4 mL of amylase solution in the fifth tube. Place all of the tubes in the 37°C water bath for 5 minutes. Obtain 5 clean droppers and label them 1-5. (To avoid contamination of these solutions
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2.1 Explain how development of children and young people is influenced by their range of personal factors. There are two main factors which affect a child and young person’s development. The first is personal factors this can be linked to genetics‚ health and learning difficulties all of which influence the development of a child. Then there is personality which is linked to the environment which is influenced later on in the development. Genetics Genetic information about a child is important
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Purpose: To find how pH and temperature levels affect the enzyme Background: Enzymes are a type of protein vital to sustaining life‚ it works with vitamins and minerals as a biological catalyst‚ which lowers the activation energy for a reaction to occur. Each individual type of enzyme does a specific job‚ and they do not die‚ they are reused. For example‚ catalase is an enzyme found in almost all living cells that will break down hydrogen peroxide and turn it into water and oxygen. The
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Enzyme Lab Report Introduction The objective of this experiment was to determine if changes in pH or temperature affected the activity of enzymes‚ specifically the enzyme sucrase. Enzymes are protein molecules that act as biological catalysts to increase the speed of the reaction or to lower the activation energy of that reaction. However‚ the activity of an enzyme can be affected by physical factors such as pH and temperature because these factors alter the structure of the enzyme (Freeman‚ 2011)
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Biology Enzyme Catalase Investigation Aim: The aim of this investigation is to study and observe whether or not the concentration of hydrogen peroxide (varying from 10 – 30 millilitres) affects the rate of reaction. Hypothesis: With the increase of the concentration of Hydrogen Peroxide 3% substrate‚ I prediction that the rate of pressure increase will begin to amplify. The pressure is bound to increase because the catalase quickly reacts with the hydrogen peroxide; this is why the more substrate
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Environmental Parameters of Enzyme Activity Alex Rocha Texas State University Abstract If you’ve ever left a cut up apple out for long‚ you’ll notice that after a while‚ it will turn brown. The reason for this is an enzyme named catechol oxidase‚ a ubiquitous plant enzymes containing a dinuclear copper center (Klabunde‚ Eicken‚ Sacchettini‚ & Krebs‚ 1998). In this experiment‚ we used two different chelators‚ ethylene diamine tetraacetic acid and phenylthiourea to test which would stop
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measure the enzyme activity of β-galactosidase in the different concentrations of o-Nitrophenylgalactoside (ONPG) using a spectrophotometer. The spectrophotometer was also set at 420nm‚ a wavelength which is best for recording the absorbance values for the experiment. From the results‚ 0.9mM ONPG solution has the highest absorbance and 0.1mM ONPG solution has the least. Also‚ 0.5mM ONPG solution has the highest rate of enzyme activity and it is the most efficient as the enzyme activity of the ONPG
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The effects of environmental temperature on respiration rates of mice were observed in this experiment. I hypothesized that when environmental temperature decreased‚ the respiration rate of mice would increase. I predicted that if environmental temperature decreased‚ the respiration rate of mice would increase. The respiration rate of the endotherm was measured using the Qubit/Vernier system and the S152 CO2 gas sensor‚ a device that measured the CO2 concentration in the air. The independent variable
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