Comment 14: Page 8: Explain if pH was controlled during fermentation. In any case‚ do a reference in the result section to the pH at the end of fermentation Response: The pH was not directly controlled during the fermentation. In the line with typical studies conducted for ABE production‚ the medium was supplemented with buffer (50 g/L KH2PO4‚ 50 g/L K2HPO4‚ 220 g/L C2H3O2NH4) as an approach to control the pH changes during the fermentation. In addition‚ it has been reported that lignocellulosic
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Type III Writing Assignment: Written in lab notebook 1. Results (recreate in notebook) 15 points/ _____ 2. Conclusion 20 points/ _____ 3. Questions 15 points/ _____ Lactase enzyme lab OBJECTIVES: 1. Understand how biological molecules are essential to the survival of living organisms * * 2. Compare the structures and functions of the major biological molecules(carbohydrates‚ proteins‚ lipids‚ and nucleic acids) as related to the survival
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cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
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Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
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Determining the Production of Glucose During Changes in Temperature and pH‚ and Manipulation of Specificity and Cofactors BIOL-1406-SL8 November 16‚ 2012 Abstract Enzymes are biological catalysts. They work by lowering the activation energy needed to initiate a chemical reaction. Enzymes work within an optimal temperature and optimal pH. Enzymes are highly specific for a single substrate. The Enzyme is usually much larger in size than the substrate it binds to. In some cases‚ an enzyme requires
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| Gram Negative Unknown | Biology 3444-006 | | Lena Wallace | 11/7/2011 | | Abstract: The purpose of this lab was to identify an unknown bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes. Introduction: This experiment was centered on metabolic and biochemical testing
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rate of respiration. I am going to change the concentration of the glucose solution and I’m going to measure the volume of gas produced during respiration in cm³. Yeast contains enzymes. Enzymes speed up a chemical reaction – they’re biological catalysts. Yeast can respire both aerobically and anaerobic; the anaerobic respiration of yeast is known as fermentation. The equation for this type of respiration in yeast is: Glucose Carbon Dioxide + Ethanol The presence of ethanol means that yeast
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Title: Cassava starch as an effective component for Ideal Biodegradable Plastic Proponent: Zhaira Morysette L. Maco‚ III-Narra student Introduction: Inventions have evolved and continue to evolve such that after several years of study‚ research and experimentation reach great developments. With continuing efforts to investigate the constituents of Philippine plants‚ we have pursued investigation of starch of the cassava plant (Manihot esculenta). Cassava tubers were gathered‚ ground and squeezed
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BioLab3 Lab Report 7 Cellular Respiration Answer Key Student Name: I. The ATP Cycle Define the following terms: Autotroph Heterotroph Aerobic respiration Anaerobic respiration Complete the chart below: Letter Defining Term A B C D II. Anaerobic Respiration Define the following terms: Alcoholic fermentation EXERCISE 1 – Alcoholic fermentation At intervals of 20‚ 40‚ and 60 minutes‚ the tubes are removed. Record the
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Osmosis The purpose of this lab is to study how membranes of plant and animal cells react when exposed to different solutions. The first experiment involves purple onion skin and the second involves rat blood in various solutions. I needed to understand certain terms before preforming this lab to be able to efficiently explain what is happening to the cells. Diffusion is the tendency of a substance to move down its concentration gradient from a more concentrated to a less concentrated area.
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