Cited: Arvidson‚ C. (n.d.). Cultivation Media for Bacteria. Retrieved December 3‚ 2014‚ from http://learn.chm.msu.edu/vibl/content/differential/ Carson‚ V. (2013). Microbiology Lab (1st ed.). Department of Cell Biology‚ Microbiology & Molecular Biology. University of South Florida. Slonczewski‚ J.‚ & Foster‚ J. (2013). Microbiology: An evolving science (Third ed.). W. W. Norton. U.S Food and Drug Administration. (2014‚ October
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with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature‚ amount of substrate (sucrose) present‚ sucrase + sucrose incubation time Effect of Temperature on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable temperature Controlled Variables pH‚ amount of substrate (sucrose) present‚ sucrase
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Weekly iLab iLab: Exploring the Microbiology Lab Section 1 Lab Safety 1. There are four safety equipment items that a lab should have. Identify two of these four items. (2 pts) Biological Safety Cabinet‚ Eyewash and shower 2. Identify one of the three ways to keep your work area safe. (1 pt) Keep your workspace free of all unnecessary materials 3. There are five recommendations for dressing properly in a lab environment. Name two of these recommendations. (2 pt) Avoid loose
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Virtual Lab Unit 3 1. Briefly describe the steps required to aseptically transfer bacteria from an unknown to a tube of liquid broth. You might have to research this using the "T" button (Tell Me More About...) To aseptically transfer bacteria from an unknown to a tube of liquid broth you have to flame the loop or wire before you begin to sterilize it. You then remove the caps from the tubes and flame the mouths of the tubes to prevent air-borne contamination. When all this is done‚ you have to
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distilled water‚ as a control‚ starch‚ glucose‚ and soft drink did not contain proteins. Egg albumin was the only test solution that contained proteins. Table 1 |Tube |Contents |Color Reaction |Conclusion | |1 |Distilled Water |Clear/Blue |Did not contain protein | |2 |Starch
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Lab: investigating hooked law with springs Purpose: to find spring constants of different springs using the slope of a graph of change in heights vs. the weight force. Also‚ to be able to understand how spring constants change when you add springs in a series or paralle Pre lab predictions: We predicted that the graph of gravitational force (mg) as a function of stretch (delta x) would look like Data: Spring #1: y = 8.2941x + 0.0685 This table represents the different distances that
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aims to produce biodegradable plastic using cassava starch as its main component. Cassava starch was mixed with water‚ epoxydized soya bean oil (ESBO)‚ glycerol‚ and polyvinyl alcohol (PVA). The mixture was then compressed and tested. Three preparations were made from the mixture. The first preparation contained 50 grams starch‚ 50 grams water‚ 50 grams PVA‚ 2.5 grams ESBO‚ and 2.5 grams glycerol. The second preparation contained 65 grams starch‚ 65 grams water‚ 50 grams PVA‚ 2.5 grams ESBO‚ and 2
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Abstract This lab was focused on determining the optimal temperature of the enzyme amylase responsible for catabolizing starch polymers and to see how different temperatures affected the rate as well as how effectively the enzyme worked. To proceed with the experiment the group set up four different test tubes for each‚ bacteria and fungal amylase‚ and labeled them accordingly with different temperatures as well as different solutions . Then the spot plates were placed on the time and temperature
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CONDITIONS FOR STARCH HYDROLYSIS THROUGH THERMOSTABLE α - AMYLASE T. Kolusheva‚ A. Marinova University of Chemical Technology and Metallurgy 8 Kl. Ohridski‚ 1756 Sofia‚ Bulgaria E-mail: e-mail: manahova@abv.bg. Received 10 July 2006 Accepted 12 November 2006 ABSTRACT The present work determines the optimal conditions for starch hydrolysis by thermostable α -amylase (EC 3.2.1.1) produced by Bac.subtilis strain XÊ-86. The hydrolysis reaction has the greatest rate at pH = 7.0‚ starch substrate
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Taylor Hill Lab 6: Enzymes Purpose: The purpose of this lab is to test for enzyme activity‚ look at enzyme specificity‚ and how temperature affects enzyme activity. Hypothesis: I believe that the room temperature activity will produce the most activity. Materials: 3% hydrogen peroxide‚ a household blender‚ potatoes (3 medium size potatoes‚ that are peeled and quartered)‚ tap water‚ 8 test tubes‚ 8 transfer pipets‚ sugar packets‚ salt‚ a small saucepan‚ a refrigerator with a freezer‚ stove
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