b. The structure of the molecule undergoing diffusion (eg. steric conformation‚ size‚ polarity‚ amount and strength of hydrogen bonding) 2. Urea was not able to diffuse through the 20 MWCO because the pores of the membrane were too small for the urea to pass through. The molecular weight of urea is 60.06 g/mol‚ over three times greater than the 20 MWCO. 3. Glucose was able to diffuse through the 200 MWCO pore because it was small enough‚ having a molecular weight of 180.2 g/mol. Albumin was
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ACTIVITY 1 Simulating Dialysis (Simple Diffusion) 1. Describe two variables that affect the rate of diffusion. __The size and the concentration. ___ 2. Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? __ The molecules were to large to go through. My results matched._ 3. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your
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Cell Structure and Function 16 October 2014 Gel Filtration and Electrophoresis Objective The essential goal of the experiment was to separate proteins in a solution based on size in different fractions. The relative protein content for each one fraction was found through the utilization of an amido black-based protein assay. Later in the trial polyacrylamide gel electrophoresis was utilized to separate BSA from hemoglobin. Methods I. Gel Filtration and Protein Assay: 1. A slurry of Bio-Gel P-100
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passive filtration of plasma through the pores that are found in the glomerulus. The plasma is forced through these pores by hydrostatic pressure. The only things that determines if a molecule will pass through the pores of the glomerulus is it’s molecular weight. The lower the molecular weight‚ the easier it will pass through the pores. Another determining factor will be if a molecule is bound to a large molecule. If this is true then passage through the pores will be hindered by the size of the
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phases ‚ two gas phases or a mixture of a gas phase and liquid phase. In industries‚ many companies have high demand on both quality and quantity of water for production. Membrane process can be used to separate chemical substances in various types and sizes from microscopic to molecular level. The objective of this experiment is to study the characteristic of four different types of membrane. The membranes used in this experiment are polyamide film ( AFC 99 ) as membrane one ‚ polyamide film ( AFC 40
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MICROBIOLOGY STUDY QUESTI0NS - EXAM 1 1. Briefly discuss the theory of spontaneous generation. What scientific evidence was presented to disprove the theory? Spontaneous generation- theory (until second half of 19th century) that some forms of life can arise spontaneously from nonliving matter‚ i.e. maggots from corpses Redi (1668)- demonstrated maggots do not arise from decaying meat by filling two jars with decaying meat (one sealed‚ one unsealed). Unsealed jar developed larvae and sealed did
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weight? ______________________________________ Which solute displayed the highest rate of diffusion through the 200 MWCO membrane? __________________________ Using the data from Chart 1‚ explain the relationship between the rate of diffusion and the size of the solute. Facilitated Diffusion 2. The following refer to Activity 2: Simulating Facilitated Diffusion. Did any of the substances travel against their concentration gradient? Explain why or why not. Using your results from Chart 2‚ what was
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Mechanisms and Permeability Name __Christopher Scott_ Lab Time/Date ___1/15/2014 Activity 1 Simulating Dialysis (Simple Diffusion) 1. Describe two variables that affect the rate of diffusion. ___ Two variables that affect rate of diffusion are molecule size and plasma membrane composition. A larger molecule will diffuse more slowly than a smaller molecule. The lipid content of the plasma membrane determines permeability of certain molecules. A plasma membrane with a higher lipid content is less permeable
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Bengal- 743122 Nearest Railway Station- Palta. Distance from Railway Station to Exam Centre 0.20Kms Kolkata Date of Examination: 24/2/2013 Time of Reporting: 8 A M Time of Examination: 10 A M Signature Thum b Im pre ssion Paste Attested Passport Size Photograph (Do Not Staple) C andidate Photo DECLARATION I ‚_ _ _ _ _ _ _ _ _ _ _ __ _ _ _ _ Son / D aughter of _ _ _ _ _ _ __ _ _ hereby dec lare and s olemnly affirm that all the partic ulars s tated in the A pplic ation form that was s ubmitted
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1a. Two reasons that the mass of the bag levels off with time include (1) equilibrium and (2) hydrostatic pressure. Equilibrium refers to the concentrations becoming equal. If there is no longer a higher concentration and lower concentration between the water and sucrose‚ osmosis can no longer take place. Osmosis can only occur when water is traveling from a higher concentrated area to a lower concentrated area. Hydrostatic pressure occurs when the bag reaches maximum capacity. Water will enter the
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