In the first part of this lab‚ E.coli cells were transformed with an R-plasmid carrying a tetracycline resistant gene‚ giving rise to tetracycline resistant E.coli strain. This was accomplished through transformation‚ which allowed E.coli to directly uptake the naked DNA molecule carrying the antibiotic resistant gene (1). However‚ in order to take up the DNA and incorporate them into their genome via recombination‚ cells must be competent (1). Therefore‚ E.coli cells which are not competent under
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Nathaniel Hawthorne’s Impression on Puritan Society Back in the day during colonial times‚ law and religion were inseparable. When a woman cheated on her husband‚ she had to be punished by law‚ even if her husband had been missing for two years and she had not thought that she would ever see him again. The heroine of the novel “Scarlet letter”‚ Hester‚ cheats on her husband. Her punishment is to wear a scarlet letter “A” on her clothing for the world to see. The “A” stands for Adultery
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Children Toys Impression Children’s toys do create social and emotional problems due to the design of the toy‚ the level of violence‚ and the message the toy sends mentally. Without realizing it‚ the design of a toy is a way of creating emotional problems among children. First‚ let’s start off with Barbie. Barbie was invented in 1959 by a young lady named Ruth Handler. Barbie became one of the most distinguished dolls‚ due to her beauty and figure. Barbie was engineered with a very small waist‚
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Othello First Impression Essay Our first impression of Othello comes from a conversation between Iago‚ Roderigo‚ and Brabantio. Our second impression of him comes from Othello himself. The first two impressions conflict greatly‚ the second being more accurate than the first. Despite the way Othello is described in the first scene‚ he shows himself to be quite different in the second. Our first impression of Othello comes from a conversation between Iago‚ Roderigo‚ and Brabantio. Iago speaks of
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Impressions after Reading Jane Eyre Jane Eyre‚ written by Charlotte Bronte‚ was met with great enthusiasm and became one of the best sellers since it was published in October 1847. The story of Jane Eyre takes place in northern England in the early to mid-19th Century. It starts as the ten-year-old Jane‚ a plain but unyielding child‚ is excluded by her Aunt Reed from the domestic circle around the hearth and bullied by her handsome but unpleasant cousins. Under the suggestion
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These two English Translations of the Russian poem by Andrei Voznesenky are about a girl being rejected for the first time. The young girl’s feelings of hurt and rejection are described in terms of coldness throughout the poem. “The first ice of human hurt”. The theme that is put across from the poem is of growing up‚ and the experience of loosing someone for the first time. Both of these poems are very similar‚ but they have both been translated in two different styles. This helps to alter the meaning
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What are your impressions of Beethoven as a person? Was he a product of his times‚ a great artist‚ or a deeply troubled and conflicted individual—or all three? Support your response with specific allusions to his life and music. Clearly evident throughout his life Beethoven was not only the product of his times but also a great artist and a deeply troubled and conflicted man. Encouraged to achieve child prodigy status‚ from a very early age Beethoven felt an immense amount of pressure to succeed
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Literature English class‚ we have focused our studies on Part 2: language and mass communication. By researching this area‚ we have studied the effects that advertisements we view today have on different gender stereotypes. I have focused my ideas on the impression of male stereotypes‚ which state that a boy’s “coming of age” originates at an age in which children are still developing. Through the use of emasculation and masculinization‚ I was able to stress the importance that one’s appearance has on their
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quote‚ “‘I do‚’ said Scrooge. ‘Merry Christmas! what right have you to be merry?... You’re poor enough.’... ‘What right have you to be dismal?... You’re rich enough.’” (3) The juxtaposition in this quote shows the difference between social classes. There are two different sides put into one paragraph; the different feeling of the rich and poor. The novella does a wonderful job putting two opposing ideas into one paragraph‚ using the same format for different words. Although Scrooge saying to his nephew
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Esguerra‚ Aya Micaela Q. English1 Narrative Essay My First on Firsts Never have I ever been the risk-taker type of person. I kept on rationalizing and reasoning out that things should be done according to a valid purpose just to avoid being asked to do them. And frankly‚ I found every purpose or reason other people trying to convince me tell‚ in any way possible‚ invalid. I have escaped many adventures in life and lost opportunities that were already laid out in front of me. I practically
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