Experiment 2: IDENTIFICATION OF UNKNOWNS BY TLC AND MP IN COMBINATION Introduction: Thin layer chromatography (TLC) is one of the most valuable techniques in organic chemistry. This is a best method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that is stationary and one that is moving or mobile. Chromatography works on the principle that different compounds will have different
Free Chromatography Thin layer chromatography
Making TLC Plates from Bulk TLC Silica Gels Many TLC users prefer to use pre-coated TLC plates‚ but others because of their special needs‚ or because they need special additives or a special thickness‚ coat their own TLC plates. This paper is a quick review of what is needed for the process. Initial instructions for plate coating: If reproducible plates are needed‚ then the user should invest in a coating device from Camag or Desaga (addresses below). The devices consist of a tray that holds
Premium Thin layer chromatography Silica gel
Introduction to Business Law- Assignment 1B Advise TLC Ltd. as to any possible action they may have for misrepresentation against answer Ltd. _____________________________________________________________________ Treitel in The Law of Contract (2003) defines a contract as: “An agreement giving rise to obligations which are enforced or recognised by law. The factor which distinguishes contractual from other legal obligations is that they are based on the agreement of the contracting
Premium Contract
Title: Thin-Layer Chromatography Objectives: Part1: 1. To learn the technique of TLC and the visualization of colourless components. 2. To identify an unknown drug by a TLC comparison with standard compounds. Part 2: To learn the separation technique by using Thin Layer Chromatography plate in separating a mixture of compounds into individual pure compound by using Spinach Leaf. Introduction: (i) General Concepts Chromatography is a common and powerful method used to separate and analyze complex
Premium Chromatography Thin layer chromatography Analytical chemistry
Abstract: Saponification is an esterification process that is used to make so from the hydrolysis of fats and oils. This process have the glycerol molecule‚ and a sodium carboxylate salt in most case sodium stearate being formed‚ however this salt depends on the base being used. In the experiment 23ml of vegetable oil was mixed with 20ml of ethyl alcohol along with 20 ml of 25% sodium hydroxide in a 250-mL Erlenmeyer flask. The content was then heated on a water bath whilst gently mixing with
Premium Sodium hydroxide Ester
technique of TLC and the visualization of colourless components. (ii)Identify the unknown drug by a TCL comparison with standard components. Part2 :to learn the separation technique by using TLC plate in separating a mixture of compound by using spinach leaf. Apparatus:UV lamp‚capillary ‚250 ML beaker Materials:aspirin‚acetaminophen‚caffeine‚unknown A‚unknown B‚TLC plates‚ethyl acetate‚Hexane‚acetic acid‚iodine Introduction: Procedure: A.spotting of the TLC plates 1.A TLC plates is obtained
Premium Thin layer chromatography Acetic acid
Experiment 1: TLC Analysis of Analgesic Drugs 1/23/2011 Purpose: The goal of this experiment is to test our knowledge and understanding of TLC analysis by having us do a TLC analysis of analgesics to figure out their main chemical components. Calculations: 1.) Rf = Distance spot traveled/ distance solvent traveled Results: Table 1: TLC Analysis Analgesic Drugs | Rf Value | Acetaminophen | 0.323 | Aspirin | 0.597 | Caffeine | 0.081 | Unknown 154 (Plate 1) | 0.081‚ 0.306‚ 0.597
Premium Aspirin Codeine Paracetamol
Introduction Chromatography is a technique used to separate a mixture into its individual components. In this experiment‚ chromatography is done by using a thin layer chromatography (TLC) plate and filter paper. The word ‘chromatography’ itself means the separation of colours. However‚ nowadays‚ both coloured and colourless compound are used in chromatography by various methods. In chromatography‚ the separation process of mixture is mainly based upon the polarity and molecular weight of sample
Premium Management Learning German language
Consulting Firm Transitional Life Care (TLC) Consulting Firm is the creation of Team B. This paper summarizes the type of consulting firm and the services TLC offers. Each team member’s roles in the firm and her duties and responsibilities are described. In addition‚ a detailed analysis of how the content and concepts from five undergraduate core courses facilitated the analysis‚ development‚ and preparation of TLC Consulting Firm. Mission Statement Transitional Life Care Consultant Firm’s mission
Premium Management Marketing Ethics
In this experiment thin-layer chromatography (TLC) was used to determine the composition of an unknown mixture of analgesics given five references of AC‚ ASP‚ CAF‚ IBU‚ SAL‚ an unknown consisting of a mixture of three of the references‚ and two unknowns (6‚23) containing a single reference compound. The composition of the unknown mixture and two single unknowns were determined by spotting the reference compounds and the unknown compounds on two TLC plates. Placed them in a developing chamber that
Premium Thin layer chromatography Acetic acid